Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15435 and RS_RS15440 are separated by 46 nucleotides RS_RS15440 and RS_RS15445 are separated by 253 nucleotides RS_RS15445 and RS_RS15450 overlap by 4 nucleotides
RS_RS15435: RS_RS15435 - hypothetical protein, at 3,311,271 to 3,311,501
_RS15435
RS_RS15440: RS_RS15440 - hypothetical protein, at 3,311,548 to 3,311,769
_RS15440
RS_RS15445: RS_RS15445 - hypothetical protein, at 3,312,023 to 3,313,184
_RS15445
RS_RS15450: RS_RS15450 - cation transporter, at 3,313,181 to 3,314,359
_RS15450
Position (kb)
3312
3313
3314 Strain fitness (log2 ratio)
-1
0
1
2 at 3311.040 kb on + strand at 3311.040 kb on + strand at 3311.041 kb on - strand at 3311.041 kb on - strand at 3311.041 kb on - strand at 3311.041 kb on - strand at 3311.041 kb on - strand at 3311.091 kb on + strand at 3311.092 kb on - strand at 3311.092 kb on - strand at 3311.092 kb on - strand at 3311.272 kb on + strand at 3311.272 kb on + strand at 3311.272 kb on + strand at 3311.272 kb on + strand at 3311.272 kb on + strand at 3311.273 kb on - strand at 3311.273 kb on - strand at 3311.351 kb on - strand, within RS_RS15435 at 3311.351 kb on - strand, within RS_RS15435 at 3311.367 kb on + strand, within RS_RS15435 at 3311.367 kb on + strand, within RS_RS15435 at 3311.492 kb on - strand at 3311.511 kb on + strand at 3311.512 kb on - strand at 3311.549 kb on + strand at 3311.549 kb on + strand at 3311.550 kb on - strand at 3311.550 kb on - strand at 3312.248 kb on + strand, within RS_RS15445 at 3312.248 kb on + strand, within RS_RS15445 at 3312.248 kb on + strand, within RS_RS15445 at 3312.248 kb on + strand, within RS_RS15445 at 3312.248 kb on + strand, within RS_RS15445 at 3312.249 kb on - strand, within RS_RS15445 at 3312.249 kb on - strand, within RS_RS15445 at 3312.422 kb on + strand, within RS_RS15445 at 3312.422 kb on + strand, within RS_RS15445 at 3312.422 kb on + strand, within RS_RS15445 at 3312.423 kb on - strand, within RS_RS15445 at 3312.524 kb on + strand, within RS_RS15445 at 3312.524 kb on + strand, within RS_RS15445 at 3312.525 kb on - strand, within RS_RS15445 at 3312.584 kb on + strand, within RS_RS15445 at 3312.585 kb on - strand, within RS_RS15445 at 3312.597 kb on - strand, within RS_RS15445 at 3312.631 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.765 kb on + strand, within RS_RS15445 at 3312.768 kb on + strand, within RS_RS15445 at 3312.768 kb on + strand, within RS_RS15445 at 3312.768 kb on + strand, within RS_RS15445 at 3312.768 kb on + strand, within RS_RS15445 at 3312.774 kb on + strand, within RS_RS15445 at 3312.774 kb on + strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.775 kb on - strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.942 kb on + strand, within RS_RS15445 at 3312.943 kb on - strand, within RS_RS15445 at 3312.943 kb on - strand, within RS_RS15445 at 3312.967 kb on + strand, within RS_RS15445 at 3312.967 kb on + strand, within RS_RS15445 at 3312.967 kb on + strand, within RS_RS15445 at 3313.100 kb on - strand at 3313.344 kb on + strand, within RS_RS15450 at 3313.345 kb on - strand, within RS_RS15450 at 3313.345 kb on - strand, within RS_RS15450 at 3313.345 kb on - strand, within RS_RS15450 at 3313.449 kb on + strand, within RS_RS15450 at 3313.449 kb on + strand, within RS_RS15450 at 3313.678 kb on + strand, within RS_RS15450 at 3313.929 kb on + strand, within RS_RS15450 at 3313.930 kb on - strand, within RS_RS15450 at 3314.102 kb on - strand, within RS_RS15450 at 3314.116 kb on - strand, within RS_RS15450 at 3314.116 kb on - strand, within RS_RS15450 at 3314.116 kb on - strand, within RS_RS15450
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,311,040 + +1.4 3,311,040 + -0.1 3,311,041 - +1.0 3,311,041 - -0.5 3,311,041 - -0.1 3,311,041 - -0.3 3,311,041 - -0.4 3,311,091 + +0.0 3,311,092 - -0.6 3,311,092 - -1.2 3,311,092 - +0.1 3,311,272 + -0.3 3,311,272 + +0.1 3,311,272 + -0.1 3,311,272 + +0.1 3,311,272 + +0.2 3,311,273 - -0.1 3,311,273 - +0.2 3,311,351 - RS_RS15435 0.35 +0.2 3,311,351 - RS_RS15435 0.35 -0.5 3,311,367 + RS_RS15435 0.42 +0.4 3,311,367 + RS_RS15435 0.42 +0.1 3,311,492 - -0.3 3,311,511 + -0.6 3,311,512 - -0.6 3,311,549 + -0.2 3,311,549 + +0.3 3,311,550 - -0.1 3,311,550 - +2.4 3,312,248 + RS_RS15445 0.19 +0.2 3,312,248 + RS_RS15445 0.19 -0.7 3,312,248 + RS_RS15445 0.19 +0.4 3,312,248 + RS_RS15445 0.19 +0.2 3,312,248 + RS_RS15445 0.19 -0.1 3,312,249 - RS_RS15445 0.19 +0.7 3,312,249 - RS_RS15445 0.19 -0.1 3,312,422 + RS_RS15445 0.34 -0.2 3,312,422 + RS_RS15445 0.34 -0.7 3,312,422 + RS_RS15445 0.34 -0.1 3,312,423 - RS_RS15445 0.34 -0.2 3,312,524 + RS_RS15445 0.43 +1.4 3,312,524 + RS_RS15445 0.43 -1.1 3,312,525 - RS_RS15445 0.43 -0.0 3,312,584 + RS_RS15445 0.48 -0.0 3,312,585 - RS_RS15445 0.48 +0.2 3,312,597 - RS_RS15445 0.49 -0.3 3,312,631 + RS_RS15445 0.52 +0.4 3,312,765 + RS_RS15445 0.64 +1.4 3,312,765 + RS_RS15445 0.64 -0.2 3,312,765 + RS_RS15445 0.64 +0.4 3,312,765 + RS_RS15445 0.64 -0.9 3,312,765 + RS_RS15445 0.64 -0.4 3,312,765 + RS_RS15445 0.64 -0.3 3,312,768 + RS_RS15445 0.64 -0.6 3,312,768 + RS_RS15445 0.64 +0.5 3,312,768 + RS_RS15445 0.64 +1.4 3,312,768 + RS_RS15445 0.64 -0.2 3,312,774 + RS_RS15445 0.65 -0.6 3,312,774 + RS_RS15445 0.65 -0.3 3,312,775 - RS_RS15445 0.65 -0.0 3,312,775 - RS_RS15445 0.65 +1.4 3,312,775 - RS_RS15445 0.65 +0.1 3,312,775 - RS_RS15445 0.65 -0.5 3,312,775 - RS_RS15445 0.65 +0.4 3,312,775 - RS_RS15445 0.65 -0.1 3,312,942 + RS_RS15445 0.79 -0.5 3,312,942 + RS_RS15445 0.79 -0.5 3,312,942 + RS_RS15445 0.79 +2.4 3,312,942 + RS_RS15445 0.79 +1.4 3,312,942 + RS_RS15445 0.79 -0.3 3,312,942 + RS_RS15445 0.79 +1.4 3,312,943 - RS_RS15445 0.79 -0.1 3,312,943 - RS_RS15445 0.79 +1.1 3,312,967 + RS_RS15445 0.81 -0.4 3,312,967 + RS_RS15445 0.81 -0.0 3,312,967 + RS_RS15445 0.81 -0.3 3,313,100 - +0.1 3,313,344 + RS_RS15450 0.14 -0.7 3,313,345 - RS_RS15450 0.14 +0.4 3,313,345 - RS_RS15450 0.14 -0.0 3,313,345 - RS_RS15450 0.14 +0.1 3,313,449 + RS_RS15450 0.23 -0.2 3,313,449 + RS_RS15450 0.23 +0.1 3,313,678 + RS_RS15450 0.42 -0.3 3,313,929 + RS_RS15450 0.63 -0.7 3,313,930 - RS_RS15450 0.64 -0.1 3,314,102 - RS_RS15450 0.78 -1.3 3,314,116 - RS_RS15450 0.79 -0.0 3,314,116 - RS_RS15450 0.79 -0.2 3,314,116 - RS_RS15450 0.79 +0.4
Or see this region's nucleotide sequence