Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS22890

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS22885 and RS_RS22890 are separated by 181 nucleotidesRS_RS22890 and RS_RS22895 are separated by 57 nucleotidesRS_RS22895 and RS_RS22900 are separated by 120 nucleotides RS_RS22885: RS_RS22885 - DNA-binding response regulator, at 1,514,239 to 1,514,463 _RS22885 RS_RS22890: RS_RS22890 - glycosyltransferase family 39 protein, at 1,514,645 to 1,516,300 _RS22890 RS_RS22895: RS_RS22895 - DUF333 domain-containing protein, at 1,516,358 to 1,516,786 _RS22895 RS_RS22900: RS_RS22900 - two-component sensor histidine kinase, at 1,516,907 to 1,518,193 _RS22900 Position (kb) 1514 1515 1516 1517Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1513.909 kb on + strandat 1513.909 kb on + strandat 1513.910 kb on - strandat 1513.910 kb on - strandat 1513.910 kb on - strandat 1513.910 kb on - strandat 1513.944 kb on + strandat 1513.944 kb on + strandat 1513.944 kb on + strandat 1513.945 kb on - strandat 1513.945 kb on - strandat 1513.945 kb on - strandat 1513.945 kb on - strandat 1513.945 kb on - strandat 1514.049 kb on + strandat 1514.050 kb on - strandat 1514.141 kb on - strandat 1514.141 kb on - strandat 1514.425 kb on + strand, within RS_RS22885at 1514.426 kb on - strand, within RS_RS22885at 1514.462 kb on - strandat 1514.558 kb on + strandat 1514.558 kb on + strandat 1514.744 kb on + strandat 1514.744 kb on + strandat 1514.837 kb on + strand, within RS_RS22890at 1515.011 kb on + strand, within RS_RS22890at 1515.011 kb on + strand, within RS_RS22890at 1515.011 kb on + strand, within RS_RS22890at 1515.012 kb on - strand, within RS_RS22890at 1515.221 kb on + strand, within RS_RS22890at 1515.222 kb on - strand, within RS_RS22890at 1515.287 kb on + strand, within RS_RS22890at 1515.287 kb on + strand, within RS_RS22890at 1515.419 kb on + strand, within RS_RS22890at 1515.419 kb on + strand, within RS_RS22890at 1515.420 kb on - strand, within RS_RS22890at 1515.612 kb on - strand, within RS_RS22890at 1515.613 kb on + strand, within RS_RS22890at 1515.614 kb on - strand, within RS_RS22890at 1515.614 kb on - strand, within RS_RS22890at 1515.614 kb on - strand, within RS_RS22890at 1516.031 kb on + strand, within RS_RS22890at 1516.032 kb on - strand, within RS_RS22890at 1516.064 kb on + strand, within RS_RS22890at 1516.064 kb on + strand, within RS_RS22890at 1516.064 kb on + strand, within RS_RS22890at 1516.064 kb on + strand, within RS_RS22890at 1516.065 kb on - strand, within RS_RS22890at 1516.065 kb on - strand, within RS_RS22890at 1516.065 kb on - strand, within RS_RS22890at 1516.065 kb on - strand, within RS_RS22890at 1516.067 kb on + strand, within RS_RS22890at 1516.068 kb on - strand, within RS_RS22890at 1516.068 kb on - strand, within RS_RS22890at 1516.068 kb on - strand, within RS_RS22890at 1516.196 kb on + strandat 1516.197 kb on - strandat 1516.197 kb on - strandat 1516.354 kb on - strandat 1516.359 kb on + strandat 1516.359 kb on + strandat 1516.360 kb on - strandat 1516.360 kb on - strandat 1516.488 kb on + strand, within RS_RS22895at 1516.488 kb on + strand, within RS_RS22895at 1516.488 kb on + strand, within RS_RS22895at 1516.488 kb on + strand, within RS_RS22895at 1516.489 kb on - strand, within RS_RS22895at 1516.489 kb on - strand, within RS_RS22895at 1516.489 kb on - strand, within RS_RS22895at 1516.489 kb on - strand, within RS_RS22895at 1516.876 kb on + strandat 1516.876 kb on + strandat 1516.877 kb on - strandat 1516.887 kb on + strandat 1516.888 kb on - strandat 1517.202 kb on + strand, within RS_RS22900at 1517.203 kb on - strand, within RS_RS22900at 1517.247 kb on + strand, within RS_RS22900at 1517.248 kb on - strand, within RS_RS22900

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,513,909 + +0.5
1,513,909 + -2.1
1,513,910 - -1.1
1,513,910 - +0.1
1,513,910 - +0.9
1,513,910 - -0.2
1,513,944 + -0.5
1,513,944 + -0.0
1,513,944 + -0.0
1,513,945 - +0.2
1,513,945 - +0.1
1,513,945 - +0.3
1,513,945 - -0.1
1,513,945 - -0.4
1,514,049 + -0.1
1,514,050 - +0.9
1,514,141 - -0.1
1,514,141 - -0.1
1,514,425 + RS_RS22885 0.83 -1.7
1,514,426 - RS_RS22885 0.83 +0.9
1,514,462 - +0.6
1,514,558 + -0.1
1,514,558 + +0.1
1,514,744 + -5.4
1,514,744 + -6.2
1,514,837 + RS_RS22890 0.12 -6.2
1,515,011 + RS_RS22890 0.22 -7.2
1,515,011 + RS_RS22890 0.22 -6.4
1,515,011 + RS_RS22890 0.22 -5.8
1,515,012 - RS_RS22890 0.22 -3.2
1,515,221 + RS_RS22890 0.35 -6.1
1,515,222 - RS_RS22890 0.35 -6.2
1,515,287 + RS_RS22890 0.39 -0.1
1,515,287 + RS_RS22890 0.39 -5.5
1,515,419 + RS_RS22890 0.47 -5.2
1,515,419 + RS_RS22890 0.47 -4.9
1,515,420 - RS_RS22890 0.47 -3.0
1,515,612 - RS_RS22890 0.58 -6.2
1,515,613 + RS_RS22890 0.58 -6.6
1,515,614 - RS_RS22890 0.59 -6.3
1,515,614 - RS_RS22890 0.59 -6.1
1,515,614 - RS_RS22890 0.59 -5.8
1,516,031 + RS_RS22890 0.84 -2.9
1,516,032 - RS_RS22890 0.84 -4.0
1,516,064 + RS_RS22890 0.86 -3.2
1,516,064 + RS_RS22890 0.86 -0.2
1,516,064 + RS_RS22890 0.86 -3.0
1,516,064 + RS_RS22890 0.86 -7.3
1,516,065 - RS_RS22890 0.86 -5.2
1,516,065 - RS_RS22890 0.86 +0.9
1,516,065 - RS_RS22890 0.86 -1.9
1,516,065 - RS_RS22890 0.86 -6.3
1,516,067 + RS_RS22890 0.86 -5.4
1,516,068 - RS_RS22890 0.86 -6.8
1,516,068 - RS_RS22890 0.86 -5.6
1,516,068 - RS_RS22890 0.86 -4.6
1,516,196 + -5.3
1,516,197 - -5.3
1,516,197 - -6.2
1,516,354 - +0.5
1,516,359 + +0.5
1,516,359 + -0.3
1,516,360 - -0.8
1,516,360 - -0.3
1,516,488 + RS_RS22895 0.30 +1.1
1,516,488 + RS_RS22895 0.30 +1.3
1,516,488 + RS_RS22895 0.30 -0.1
1,516,488 + RS_RS22895 0.30 -0.3
1,516,489 - RS_RS22895 0.31 +0.9
1,516,489 - RS_RS22895 0.31 -0.9
1,516,489 - RS_RS22895 0.31 -0.5
1,516,489 - RS_RS22895 0.31 +0.9
1,516,876 + -0.1
1,516,876 + -0.1
1,516,877 - +0.2
1,516,887 + -1.1
1,516,888 - +0.0
1,517,202 + RS_RS22900 0.23 +0.9
1,517,203 - RS_RS22900 0.23 -0.4
1,517,247 + RS_RS22900 0.26 +0.6
1,517,248 - RS_RS22900 0.26 -0.3

Or see this region's nucleotide sequence