Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS22885 and RS_RS22890 are separated by 181 nucleotides RS_RS22890 and RS_RS22895 are separated by 57 nucleotides RS_RS22895 and RS_RS22900 are separated by 120 nucleotides
RS_RS22885: RS_RS22885 - DNA-binding response regulator, at 1,514,239 to 1,514,463
_RS22885
RS_RS22890: RS_RS22890 - glycosyltransferase family 39 protein, at 1,514,645 to 1,516,300
_RS22890
RS_RS22895: RS_RS22895 - DUF333 domain-containing protein, at 1,516,358 to 1,516,786
_RS22895
RS_RS22900: RS_RS22900 - two-component sensor histidine kinase, at 1,516,907 to 1,518,193
_RS22900
Position (kb)
1514
1515
1516
1517 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1513.909 kb on + strand at 1513.909 kb on + strand at 1513.910 kb on - strand at 1513.910 kb on - strand at 1513.910 kb on - strand at 1513.910 kb on - strand at 1513.944 kb on + strand at 1513.944 kb on + strand at 1513.944 kb on + strand at 1513.945 kb on - strand at 1513.945 kb on - strand at 1513.945 kb on - strand at 1513.945 kb on - strand at 1513.945 kb on - strand at 1514.049 kb on + strand at 1514.050 kb on - strand at 1514.141 kb on - strand at 1514.141 kb on - strand at 1514.425 kb on + strand, within RS_RS22885 at 1514.426 kb on - strand, within RS_RS22885 at 1514.462 kb on - strand at 1514.558 kb on + strand at 1514.558 kb on + strand at 1514.744 kb on + strand at 1514.744 kb on + strand at 1514.837 kb on + strand, within RS_RS22890 at 1515.011 kb on + strand, within RS_RS22890 at 1515.011 kb on + strand, within RS_RS22890 at 1515.011 kb on + strand, within RS_RS22890 at 1515.012 kb on - strand, within RS_RS22890 at 1515.221 kb on + strand, within RS_RS22890 at 1515.222 kb on - strand, within RS_RS22890 at 1515.287 kb on + strand, within RS_RS22890 at 1515.287 kb on + strand, within RS_RS22890 at 1515.419 kb on + strand, within RS_RS22890 at 1515.419 kb on + strand, within RS_RS22890 at 1515.420 kb on - strand, within RS_RS22890 at 1515.612 kb on - strand, within RS_RS22890 at 1515.613 kb on + strand, within RS_RS22890 at 1515.614 kb on - strand, within RS_RS22890 at 1515.614 kb on - strand, within RS_RS22890 at 1515.614 kb on - strand, within RS_RS22890 at 1516.031 kb on + strand, within RS_RS22890 at 1516.032 kb on - strand, within RS_RS22890 at 1516.064 kb on + strand, within RS_RS22890 at 1516.064 kb on + strand, within RS_RS22890 at 1516.064 kb on + strand, within RS_RS22890 at 1516.064 kb on + strand, within RS_RS22890 at 1516.065 kb on - strand, within RS_RS22890 at 1516.065 kb on - strand, within RS_RS22890 at 1516.065 kb on - strand, within RS_RS22890 at 1516.065 kb on - strand, within RS_RS22890 at 1516.067 kb on + strand, within RS_RS22890 at 1516.068 kb on - strand, within RS_RS22890 at 1516.068 kb on - strand, within RS_RS22890 at 1516.068 kb on - strand, within RS_RS22890 at 1516.196 kb on + strand at 1516.197 kb on - strand at 1516.197 kb on - strand at 1516.354 kb on - strand at 1516.359 kb on + strand at 1516.359 kb on + strand at 1516.360 kb on - strand at 1516.360 kb on - strand at 1516.488 kb on + strand, within RS_RS22895 at 1516.488 kb on + strand, within RS_RS22895 at 1516.488 kb on + strand, within RS_RS22895 at 1516.488 kb on + strand, within RS_RS22895 at 1516.489 kb on - strand, within RS_RS22895 at 1516.489 kb on - strand, within RS_RS22895 at 1516.489 kb on - strand, within RS_RS22895 at 1516.489 kb on - strand, within RS_RS22895 at 1516.876 kb on + strand at 1516.876 kb on + strand at 1516.877 kb on - strand at 1516.887 kb on + strand at 1516.888 kb on - strand at 1517.202 kb on + strand, within RS_RS22900 at 1517.203 kb on - strand, within RS_RS22900 at 1517.247 kb on + strand, within RS_RS22900 at 1517.248 kb on - strand, within RS_RS22900
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,513,909 + +0.5 1,513,909 + -2.1 1,513,910 - -1.1 1,513,910 - +0.1 1,513,910 - +0.9 1,513,910 - -0.2 1,513,944 + -0.5 1,513,944 + -0.0 1,513,944 + -0.0 1,513,945 - +0.2 1,513,945 - +0.1 1,513,945 - +0.3 1,513,945 - -0.1 1,513,945 - -0.4 1,514,049 + -0.1 1,514,050 - +0.9 1,514,141 - -0.1 1,514,141 - -0.1 1,514,425 + RS_RS22885 0.83 -1.7 1,514,426 - RS_RS22885 0.83 +0.9 1,514,462 - +0.6 1,514,558 + -0.1 1,514,558 + +0.1 1,514,744 + -5.4 1,514,744 + -6.2 1,514,837 + RS_RS22890 0.12 -6.2 1,515,011 + RS_RS22890 0.22 -7.2 1,515,011 + RS_RS22890 0.22 -6.4 1,515,011 + RS_RS22890 0.22 -5.8 1,515,012 - RS_RS22890 0.22 -3.2 1,515,221 + RS_RS22890 0.35 -6.1 1,515,222 - RS_RS22890 0.35 -6.2 1,515,287 + RS_RS22890 0.39 -0.1 1,515,287 + RS_RS22890 0.39 -5.5 1,515,419 + RS_RS22890 0.47 -5.2 1,515,419 + RS_RS22890 0.47 -4.9 1,515,420 - RS_RS22890 0.47 -3.0 1,515,612 - RS_RS22890 0.58 -6.2 1,515,613 + RS_RS22890 0.58 -6.6 1,515,614 - RS_RS22890 0.59 -6.3 1,515,614 - RS_RS22890 0.59 -6.1 1,515,614 - RS_RS22890 0.59 -5.8 1,516,031 + RS_RS22890 0.84 -2.9 1,516,032 - RS_RS22890 0.84 -4.0 1,516,064 + RS_RS22890 0.86 -3.2 1,516,064 + RS_RS22890 0.86 -0.2 1,516,064 + RS_RS22890 0.86 -3.0 1,516,064 + RS_RS22890 0.86 -7.3 1,516,065 - RS_RS22890 0.86 -5.2 1,516,065 - RS_RS22890 0.86 +0.9 1,516,065 - RS_RS22890 0.86 -1.9 1,516,065 - RS_RS22890 0.86 -6.3 1,516,067 + RS_RS22890 0.86 -5.4 1,516,068 - RS_RS22890 0.86 -6.8 1,516,068 - RS_RS22890 0.86 -5.6 1,516,068 - RS_RS22890 0.86 -4.6 1,516,196 + -5.3 1,516,197 - -5.3 1,516,197 - -6.2 1,516,354 - +0.5 1,516,359 + +0.5 1,516,359 + -0.3 1,516,360 - -0.8 1,516,360 - -0.3 1,516,488 + RS_RS22895 0.30 +1.1 1,516,488 + RS_RS22895 0.30 +1.3 1,516,488 + RS_RS22895 0.30 -0.1 1,516,488 + RS_RS22895 0.30 -0.3 1,516,489 - RS_RS22895 0.31 +0.9 1,516,489 - RS_RS22895 0.31 -0.9 1,516,489 - RS_RS22895 0.31 -0.5 1,516,489 - RS_RS22895 0.31 +0.9 1,516,876 + -0.1 1,516,876 + -0.1 1,516,877 - +0.2 1,516,887 + -1.1 1,516,888 - +0.0 1,517,202 + RS_RS22900 0.23 +0.9 1,517,203 - RS_RS22900 0.23 -0.4 1,517,247 + RS_RS22900 0.26 +0.6 1,517,248 - RS_RS22900 0.26 -0.3
Or see this region's nucleotide sequence