Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16710 and RS_RS16715 overlap by 15 nucleotides RS_RS16715 and RS_RS16720 are separated by 100 nucleotides RS_RS16720 and RS_RS16725 are separated by 39 nucleotides
RS_RS16710: RS_RS16710 - membrane protein, at 3,597,023 to 3,597,979
_RS16710
RS_RS16715: RS_RS16715 - phosphohydrolase, at 3,597,965 to 3,598,473
_RS16715
RS_RS16720: RS_RS16720 - membrane protein, at 3,598,574 to 3,599,332
_RS16720
RS_RS16725: RS_RS16725 - hypothetical protein, at 3,599,372 to 3,599,815
_RS16725
Position (kb)
3597
3598
3599 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3596.972 kb on - strand at 3596.972 kb on - strand at 3596.972 kb on - strand at 3597.359 kb on + strand, within RS_RS16710 at 3597.580 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.778 kb on + strand, within RS_RS16710 at 3597.857 kb on + strand, within RS_RS16710 at 3597.858 kb on - strand, within RS_RS16710 at 3598.272 kb on - strand, within RS_RS16715 at 3598.272 kb on - strand, within RS_RS16715 at 3598.496 kb on + strand at 3598.593 kb on + strand at 3598.675 kb on - strand, within RS_RS16720 at 3598.828 kb on - strand, within RS_RS16720 at 3598.893 kb on + strand, within RS_RS16720 at 3598.893 kb on + strand, within RS_RS16720 at 3598.894 kb on - strand, within RS_RS16720 at 3598.894 kb on - strand, within RS_RS16720 at 3598.995 kb on + strand, within RS_RS16720 at 3599.127 kb on + strand, within RS_RS16720 at 3599.128 kb on - strand, within RS_RS16720 at 3599.148 kb on + strand, within RS_RS16720 at 3599.149 kb on - strand, within RS_RS16720 at 3599.149 kb on - strand, within RS_RS16720
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 3,596,972 - +0.1 3,596,972 - -0.5 3,596,972 - -0.6 3,597,359 + RS_RS16710 0.35 -0.4 3,597,580 + RS_RS16710 0.58 -1.3 3,597,626 + RS_RS16710 0.63 -0.7 3,597,626 + RS_RS16710 0.63 -0.3 3,597,626 + RS_RS16710 0.63 -0.1 3,597,626 + RS_RS16710 0.63 +0.6 3,597,627 - RS_RS16710 0.63 -0.4 3,597,627 - RS_RS16710 0.63 +2.3 3,597,627 - RS_RS16710 0.63 -0.1 3,597,627 - RS_RS16710 0.63 +0.3 3,597,778 + RS_RS16710 0.79 -0.1 3,597,857 + RS_RS16710 0.87 -0.1 3,597,858 - RS_RS16710 0.87 +0.1 3,598,272 - RS_RS16715 0.60 +0.4 3,598,272 - RS_RS16715 0.60 -0.5 3,598,496 + -2.2 3,598,593 + -0.3 3,598,675 - RS_RS16720 0.13 -1.0 3,598,828 - RS_RS16720 0.33 -0.1 3,598,893 + RS_RS16720 0.42 +1.3 3,598,893 + RS_RS16720 0.42 +0.8 3,598,894 - RS_RS16720 0.42 -0.3 3,598,894 - RS_RS16720 0.42 -0.7 3,598,995 + RS_RS16720 0.55 +0.3 3,599,127 + RS_RS16720 0.73 -0.3 3,599,128 - RS_RS16720 0.73 +1.3 3,599,148 + RS_RS16720 0.76 -0.1 3,599,149 - RS_RS16720 0.76 -0.6 3,599,149 - RS_RS16720 0.76 +0.4
Or see this region's nucleotide sequence