Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10045

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10040 and RS_RS10045 are separated by 108 nucleotidesRS_RS10045 and RS_RS10050 are separated by 31 nucleotides RS_RS10040: RS_RS10040 - malate dehydrogenase, at 2,174,783 to 2,175,772 _RS10040 RS_RS10045: RS_RS10045 - CoA ester lyase, at 2,175,881 to 2,176,861 _RS10045 RS_RS10050: RS_RS10050 - methylisocitrate lyase, at 2,176,893 to 2,177,789 _RS10050 Position (kb) 2175 2176 2177Strain fitness (log2 ratio) -3 -2 -1 0 1at 2175.365 kb on + strandat 2175.388 kb on + strand, within RS_RS10040at 2175.662 kb on + strand, within RS_RS10040at 2175.662 kb on + strandat 2175.662 kb on + strand, within RS_RS10040at 2175.663 kb on - strand, within RS_RS10040at 2175.872 kb on + strandat 2175.872 kb on + strandat 2175.873 kb on - strandat 2175.873 kb on - strandat 2176.369 kb on - strand, within RS_RS10045at 2177.028 kb on + strand, within RS_RS10050at 2177.029 kb on - strand, within RS_RS10050at 2177.029 kb on - strand, within RS_RS10050at 2177.029 kb on - strand, within RS_RS10050at 2177.029 kb on - strand, within RS_RS10050at 2177.240 kb on + strand, within RS_RS10050at 2177.241 kb on - strand, within RS_RS10050at 2177.604 kb on + strand, within RS_RS10050at 2177.721 kb on + strandat 2177.722 kb on - strandat 2177.745 kb on + strandat 2177.745 kb on + strandat 2177.842 kb on - strandat 2177.842 kb on - strandat 2177.842 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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2,175,365 + -1.1
2,175,388 + RS_RS10040 0.61 -0.7
2,175,662 + RS_RS10040 0.89 -3.1
2,175,662 + +0.9
2,175,662 + RS_RS10040 0.89 -0.7
2,175,663 - RS_RS10040 0.89 -0.1
2,175,872 + -0.6
2,175,872 + +0.6
2,175,873 - -0.1
2,175,873 - +0.5
2,176,369 - RS_RS10045 0.50 -0.7
2,177,028 + RS_RS10050 0.15 -0.7
2,177,029 - RS_RS10050 0.15 -0.1
2,177,029 - RS_RS10050 0.15 +0.1
2,177,029 - RS_RS10050 0.15 +0.0
2,177,029 - RS_RS10050 0.15 +0.9
2,177,240 + RS_RS10050 0.39 +0.3
2,177,241 - RS_RS10050 0.39 +0.6
2,177,604 + RS_RS10050 0.79 +0.2
2,177,721 + +0.4
2,177,722 - -0.0
2,177,745 + +0.2
2,177,745 + +0.8
2,177,842 - -0.7
2,177,842 - -0.6
2,177,842 - +0.2

Or see this region's nucleotide sequence