Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS04735 and RS_RS04740 are separated by 482 nucleotides RS_RS04740 and RS_RS04745 are separated by 598 nucleotides
RS_RS04735: RS_RS04735 - RNA helicase, at 1,000,548 to 1,002,374
_RS04735
RS_RS04740: RS_RS04740 - hypothetical protein, at 1,002,857 to 1,003,045
_RS04740
RS_RS04745: RS_RS04745 - hypothetical protein, at 1,003,644 to 1,004,228
_RS04745
Position (kb)
1002
1003
1004 Strain fitness (log2 ratio)
-2
-1
0
1 at 1002.384 kb on + strand at 1002.385 kb on - strand at 1002.385 kb on - strand at 1002.385 kb on - strand at 1002.741 kb on + strand at 1002.815 kb on + strand at 1002.816 kb on - strand at 1002.818 kb on - strand at 1002.890 kb on + strand, within RS_RS04740 at 1003.043 kb on + strand at 1003.043 kb on + strand at 1003.044 kb on - strand at 1003.195 kb on - strand at 1003.207 kb on - strand at 1003.344 kb on - strand at 1003.344 kb on - strand at 1003.344 kb on - strand at 1003.370 kb on + strand at 1003.371 kb on - strand at 1003.448 kb on + strand at 1003.449 kb on - strand at 1003.449 kb on - strand at 1003.526 kb on + strand at 1003.527 kb on - strand at 1003.527 kb on - strand at 1003.527 kb on - strand at 1003.538 kb on - strand at 1003.752 kb on + strand, within RS_RS04745 at 1003.781 kb on + strand, within RS_RS04745 at 1003.799 kb on + strand, within RS_RS04745 at 1003.799 kb on + strand, within RS_RS04745 at 1003.800 kb on - strand, within RS_RS04745 at 1003.947 kb on + strand, within RS_RS04745 at 1003.947 kb on + strand, within RS_RS04745 at 1003.948 kb on - strand, within RS_RS04745 at 1003.948 kb on - strand, within RS_RS04745 at 1003.948 kb on - strand, within RS_RS04745 at 1004.014 kb on + strand, within RS_RS04745 at 1004.014 kb on + strand, within RS_RS04745 at 1004.014 kb on + strand, within RS_RS04745 at 1004.014 kb on + strand, within RS_RS04745 at 1004.015 kb on - strand, within RS_RS04745
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,002,384 + +1.0 1,002,385 - -0.0 1,002,385 - +0.6 1,002,385 - +0.1 1,002,741 + -0.7 1,002,815 + +0.4 1,002,816 - -0.6 1,002,818 - -0.0 1,002,890 + RS_RS04740 0.17 -2.0 1,003,043 + -0.9 1,003,043 + +0.2 1,003,044 - -0.4 1,003,195 - +0.3 1,003,207 - +0.1 1,003,344 - +0.6 1,003,344 - -0.1 1,003,344 - +1.0 1,003,370 + -0.3 1,003,371 - -0.1 1,003,448 + +0.1 1,003,449 - +0.5 1,003,449 - +1.0 1,003,526 + +0.5 1,003,527 - -0.3 1,003,527 - -0.3 1,003,527 - -0.0 1,003,538 - -1.2 1,003,752 + RS_RS04745 0.18 +0.2 1,003,781 + RS_RS04745 0.23 +0.0 1,003,799 + RS_RS04745 0.26 -0.1 1,003,799 + RS_RS04745 0.26 +0.3 1,003,800 - RS_RS04745 0.27 -0.3 1,003,947 + RS_RS04745 0.52 -1.0 1,003,947 + RS_RS04745 0.52 -0.7 1,003,948 - RS_RS04745 0.52 +1.0 1,003,948 - RS_RS04745 0.52 -1.2 1,003,948 - RS_RS04745 0.52 +0.4 1,004,014 + RS_RS04745 0.63 -0.5 1,004,014 + RS_RS04745 0.63 +0.1 1,004,014 + RS_RS04745 0.63 -0.6 1,004,014 + RS_RS04745 0.63 +0.8 1,004,015 - RS_RS04745 0.63 -0.4
Or see this region's nucleotide sequence