Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS25280 and RS_RS25285 are separated by 24 nucleotides RS_RS25285 and RS_RS01235 are separated by 682 nucleotides
RS_RS25280: RS_RS25280 - calcium binding hemolysin protein, at 277,167 to 281,666
_RS25280
RS_RS25285: RS_RS25285 - hypothetical protein, at 281,691 to 282,155
_RS25285
RS_RS01235: RS_RS01235 - IS256 family transposase ISRso7, at 282,838 to 284,088
_RS01235
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1 at 281.060 kb on + strand, within RS_RS25280 at 281.060 kb on + strand, within RS_RS25280 at 281.060 kb on + strand, within RS_RS25280 at 281.060 kb on + strand, within RS_RS25280 at 281.060 kb on + strand, within RS_RS25280 at 281.061 kb on - strand, within RS_RS25280 at 281.061 kb on - strand, within RS_RS25280 at 281.177 kb on + strand, within RS_RS25280 at 281.177 kb on + strand, within RS_RS25280 at 281.178 kb on - strand, within RS_RS25280 at 281.178 kb on - strand, within RS_RS25280 at 281.370 kb on + strand at 281.371 kb on - strand at 281.406 kb on - strand at 281.447 kb on + strand at 281.490 kb on + strand at 281.500 kb on + strand at 281.808 kb on + strand, within RS_RS25285 at 282.132 kb on + strand at 282.133 kb on - strand at 282.315 kb on - strand at 282.354 kb on - strand at 282.456 kb on - strand at 282.529 kb on + strand at 282.530 kb on - strand at 282.548 kb on + strand at 282.548 kb on + strand at 282.552 kb on + strand at 282.552 kb on + strand at 282.636 kb on + strand at 282.672 kb on + strand at 282.816 kb on - strand at 282.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 281,060 + RS_RS25280 0.87 +0.5 281,060 + RS_RS25280 0.87 -0.6 281,060 + RS_RS25280 0.87 +0.0 281,060 + RS_RS25280 0.87 -0.8 281,060 + RS_RS25280 0.87 -0.4 281,061 - RS_RS25280 0.87 -0.5 281,061 - RS_RS25280 0.87 -1.2 281,177 + RS_RS25280 0.89 -0.5 281,177 + RS_RS25280 0.89 +0.7 281,178 - RS_RS25280 0.89 +0.5 281,178 - RS_RS25280 0.89 +0.2 281,370 + -0.9 281,371 - +1.5 281,406 - -0.2 281,447 + -0.4 281,490 + +0.3 281,500 + -0.0 281,808 + RS_RS25285 0.25 -0.2 282,132 + -0.4 282,133 - +1.5 282,315 - +0.5 282,354 - +0.3 282,456 - +1.0 282,529 + +0.5 282,530 - +0.3 282,548 + -0.2 282,548 + -0.5 282,552 + +0.2 282,552 + -0.3 282,636 + +0.3 282,672 + +0.1 282,816 - +0.2 282,816 - +0.4
Or see this region's nucleotide sequence