Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS16545

Experiment: SMSA no antibiotic control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS16540 and RS_RS16545 are separated by 161 nucleotidesRS_RS16545 and RS_RS16550 are separated by 582 nucleotides RS_RS16540: RS_RS16540 - trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase, at 3,558,770 to 3,562,747 _RS16540 RS_RS16545: RS_RS16545 - primosomal protein N', at 3,562,909 to 3,565,215 _RS16545 RS_RS16550: RS_RS16550 - uroporphyrinogen decarboxylase, at 3,565,798 to 3,566,892 _RS16550 Position (kb) 3562 3563 3564 3565 3566Strain fitness (log2 ratio) -2 -1 0 1at 3562.629 kb on + strandat 3562.629 kb on + strandat 3562.629 kb on + strandat 3562.629 kb on + strandat 3562.629 kb on + strandat 3562.630 kb on - strandat 3562.630 kb on - strandat 3562.630 kb on - strandat 3562.742 kb on - strandat 3562.742 kb on - strandat 3562.805 kb on + strandat 3562.805 kb on + strandat 3562.805 kb on + strandat 3562.806 kb on - strandat 3562.837 kb on - strandat 3565.344 kb on - strandat 3565.344 kb on - strandat 3565.475 kb on - strandat 3565.594 kb on + strandat 3565.595 kb on - strandat 3565.776 kb on - strandat 3565.799 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA no antibiotic control
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3,562,629 + +0.5
3,562,629 + +0.3
3,562,629 + -0.0
3,562,629 + -1.1
3,562,629 + -0.0
3,562,630 - -0.0
3,562,630 - -0.0
3,562,630 - +0.2
3,562,742 - +0.0
3,562,742 - +0.0
3,562,805 + +0.6
3,562,805 + -0.1
3,562,805 + -2.1
3,562,806 - -0.8
3,562,837 - +0.2
3,565,344 - -0.1
3,565,344 - +0.3
3,565,475 - +0.8
3,565,594 + +0.2
3,565,595 - -0.0
3,565,776 - +0.1
3,565,799 + +0.1

Or see this region's nucleotide sequence