Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS22365 and RS_RS22375 overlap by 8 nucleotides RS_RS22375 and RS_RS22380 are separated by 51 nucleotides RS_RS22380 and RS_RS22385 are separated by 77 nucleotides
RS_RS22365: RS_RS22365 - membrane protein, at 1,379,151 to 1,380,515
_RS22365
RS_RS22375: RS_RS22375 - Flp pilus assembly protein CpaB, at 1,380,508 to 1,381,448
_RS22375
RS_RS22380: RS_RS22380 - pilus assembly protein, at 1,381,500 to 1,381,991
_RS22380
RS_RS22385: RS_RS22385 - peptidase, at 1,382,069 to 1,382,628
_RS22385
Position (kb)
1380
1381
1382 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1379.641 kb on + strand, within RS_RS22365 at 1379.641 kb on + strand, within RS_RS22365 at 1379.887 kb on + strand, within RS_RS22365 at 1379.888 kb on - strand, within RS_RS22365 at 1379.888 kb on - strand, within RS_RS22365 at 1379.888 kb on - strand, within RS_RS22365 at 1379.888 kb on - strand, within RS_RS22365 at 1380.115 kb on + strand, within RS_RS22365 at 1380.115 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.207 kb on + strand, within RS_RS22365 at 1380.208 kb on - strand, within RS_RS22365 at 1380.208 kb on - strand, within RS_RS22365 at 1380.208 kb on - strand, within RS_RS22365 at 1380.208 kb on - strand, within RS_RS22365 at 1380.208 kb on - strand, within RS_RS22365 at 1380.214 kb on + strand, within RS_RS22365 at 1380.214 kb on + strand, within RS_RS22365 at 1380.214 kb on + strand, within RS_RS22365 at 1381.020 kb on + strand, within RS_RS22375 at 1381.020 kb on + strand, within RS_RS22375 at 1381.020 kb on + strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.021 kb on - strand, within RS_RS22375 at 1381.030 kb on + strand, within RS_RS22375 at 1381.030 kb on + strand, within RS_RS22375 at 1381.031 kb on - strand, within RS_RS22375 at 1381.732 kb on + strand, within RS_RS22380 at 1381.732 kb on + strand, within RS_RS22380 at 1381.733 kb on - strand, within RS_RS22380 at 1381.840 kb on + strand, within RS_RS22380 at 1381.841 kb on - strand, within RS_RS22380 at 1381.841 kb on - strand, within RS_RS22380 at 1381.844 kb on - strand, within RS_RS22380 at 1381.844 kb on - strand, within RS_RS22380 at 1381.844 kb on - strand, within RS_RS22380 at 1381.844 kb on - strand, within RS_RS22380 at 1382.007 kb on + strand at 1382.070 kb on + strand at 1382.070 kb on + strand at 1382.070 kb on + strand at 1382.070 kb on + strand at 1382.070 kb on + strand at 1382.071 kb on - strand at 1382.071 kb on - strand at 1382.071 kb on - strand at 1382.071 kb on - strand at 1382.071 kb on - strand at 1382.142 kb on + strand, within RS_RS22385 at 1382.142 kb on + strand, within RS_RS22385 at 1382.142 kb on + strand, within RS_RS22385 at 1382.143 kb on - strand, within RS_RS22385 at 1382.143 kb on - strand, within RS_RS22385 at 1382.143 kb on - strand, within RS_RS22385 at 1382.143 kb on - strand, within RS_RS22385 at 1382.152 kb on - strand, within RS_RS22385 at 1382.152 kb on - strand, within RS_RS22385 at 1382.402 kb on + strand, within RS_RS22385 at 1382.403 kb on - strand, within RS_RS22385
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,379,641 + RS_RS22365 0.36 -1.4 1,379,641 + RS_RS22365 0.36 -1.0 1,379,887 + RS_RS22365 0.54 -0.6 1,379,888 - RS_RS22365 0.54 +0.1 1,379,888 - RS_RS22365 0.54 +0.3 1,379,888 - RS_RS22365 0.54 +0.7 1,379,888 - RS_RS22365 0.54 +0.7 1,380,115 + RS_RS22365 0.71 -0.0 1,380,115 + RS_RS22365 0.71 -0.3 1,380,207 + RS_RS22365 0.77 +0.1 1,380,207 + RS_RS22365 0.77 -0.8 1,380,207 + RS_RS22365 0.77 +2.3 1,380,207 + RS_RS22365 0.77 +3.0 1,380,207 + RS_RS22365 0.77 +0.7 1,380,207 + RS_RS22365 0.77 -0.5 1,380,207 + RS_RS22365 0.77 +0.3 1,380,208 - RS_RS22365 0.77 +2.0 1,380,208 - RS_RS22365 0.77 -0.3 1,380,208 - RS_RS22365 0.77 -0.3 1,380,208 - RS_RS22365 0.77 +0.2 1,380,208 - RS_RS22365 0.77 -1.0 1,380,214 + RS_RS22365 0.78 +0.9 1,380,214 + RS_RS22365 0.78 -0.5 1,380,214 + RS_RS22365 0.78 +1.2 1,381,020 + RS_RS22375 0.54 -1.2 1,381,020 + RS_RS22375 0.54 +0.2 1,381,020 + RS_RS22375 0.54 +1.0 1,381,021 - RS_RS22375 0.55 -0.4 1,381,021 - RS_RS22375 0.55 -0.3 1,381,021 - RS_RS22375 0.55 -0.5 1,381,021 - RS_RS22375 0.55 -0.4 1,381,021 - RS_RS22375 0.55 +0.2 1,381,021 - RS_RS22375 0.55 +1.0 1,381,030 + RS_RS22375 0.55 +0.5 1,381,030 + RS_RS22375 0.55 +0.4 1,381,031 - RS_RS22375 0.56 -0.2 1,381,732 + RS_RS22380 0.47 -0.3 1,381,732 + RS_RS22380 0.47 -0.5 1,381,733 - RS_RS22380 0.47 +1.3 1,381,840 + RS_RS22380 0.69 -1.0 1,381,841 - RS_RS22380 0.69 +0.6 1,381,841 - RS_RS22380 0.69 -0.8 1,381,844 - RS_RS22380 0.70 -0.0 1,381,844 - RS_RS22380 0.70 -0.1 1,381,844 - RS_RS22380 0.70 -1.0 1,381,844 - RS_RS22380 0.70 +0.5 1,382,007 + +0.3 1,382,070 + +0.1 1,382,070 + -0.1 1,382,070 + +0.4 1,382,070 + -0.3 1,382,070 + +0.1 1,382,071 - -0.3 1,382,071 - +0.7 1,382,071 - +1.0 1,382,071 - +1.1 1,382,071 - -0.1 1,382,142 + RS_RS22385 0.13 -0.9 1,382,142 + RS_RS22385 0.13 +0.4 1,382,142 + RS_RS22385 0.13 +0.4 1,382,143 - RS_RS22385 0.13 +0.3 1,382,143 - RS_RS22385 0.13 -0.5 1,382,143 - RS_RS22385 0.13 +0.4 1,382,143 - RS_RS22385 0.13 -0.7 1,382,152 - RS_RS22385 0.15 +2.0 1,382,152 - RS_RS22385 0.15 +0.7 1,382,402 + RS_RS22385 0.59 -0.3 1,382,403 - RS_RS22385 0.60 +0.0
Or see this region's nucleotide sequence