Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS18135 and RS_RS18140 are separated by 119 nucleotides RS_RS18140 and RS_RS18145 are separated by 76 nucleotides RS_RS18145 and RS_RS18150 overlap by 4 nucleotides
RS_RS18135: RS_RS18135 - hypothetical protein, at 253,487 to 254,530
_RS18135
RS_RS18140: RS_RS18140 - IS5/IS1182 family transposase, at 254,650 to 255,636
_RS18140
RS_RS18145: RS_RS18145 - hypothetical protein, at 255,713 to 256,201
_RS18145
RS_RS18150: RS_RS18150 - hypothetical protein, at 256,198 to 257,904
_RS18150
Position (kb) 254
255
256 Strain fitness (log2 ratio) -2
-1
0
1
2 at 254.070 kb on + strand, within RS_RS18135 at 254.127 kb on + strand, within RS_RS18135 at 254.201 kb on + strand, within RS_RS18135 at 254.202 kb on - strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.239 kb on + strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.240 kb on - strand, within RS_RS18135 at 254.392 kb on + strand, within RS_RS18135 at 254.497 kb on + strand at 254.498 kb on - strand at 254.499 kb on + strand at 254.499 kb on + strand at 254.499 kb on + strand at 254.499 kb on + strand at 254.500 kb on - strand at 254.500 kb on - strand at 255.709 kb on + strand at 255.733 kb on + strand at 255.734 kb on - strand at 255.734 kb on - strand at 255.734 kb on - strand at 255.735 kb on + strand at 255.735 kb on + strand at 255.735 kb on + strand at 255.736 kb on - strand at 255.751 kb on - strand at 255.801 kb on - strand, within RS_RS18145 at 255.801 kb on - strand, within RS_RS18145 at 255.823 kb on + strand, within RS_RS18145 at 255.823 kb on + strand, within RS_RS18145 at 255.825 kb on + strand, within RS_RS18145 at 255.855 kb on + strand, within RS_RS18145 at 255.856 kb on - strand, within RS_RS18145 at 255.897 kb on + strand, within RS_RS18145 at 255.898 kb on - strand, within RS_RS18145 at 255.953 kb on + strand, within RS_RS18145 at 256.066 kb on + strand, within RS_RS18145 at 256.067 kb on - strand, within RS_RS18145 at 256.067 kb on - strand, within RS_RS18145 at 256.079 kb on - strand, within RS_RS18145 at 256.079 kb on - strand, within RS_RS18145 at 256.109 kb on + strand, within RS_RS18145 at 256.110 kb on - strand, within RS_RS18145 at 256.110 kb on - strand, within RS_RS18145 at 256.110 kb on - strand, within RS_RS18145 at 256.117 kb on - strand, within RS_RS18145 at 256.234 kb on + strand at 256.234 kb on + strand at 256.234 kb on + strand at 256.234 kb on + strand at 256.234 kb on + strand at 256.235 kb on - strand at 256.235 kb on - strand at 256.235 kb on - strand at 256.235 kb on - strand at 256.244 kb on + strand at 256.244 kb on + strand at 256.244 kb on + strand at 256.277 kb on + strand at 256.277 kb on + strand at 256.277 kb on + strand at 256.355 kb on + strand at 256.556 kb on + strand, within RS_RS18150 at 256.565 kb on + strand, within RS_RS18150 at 256.565 kb on + strand, within RS_RS18150 at 256.565 kb on + strand, within RS_RS18150 at 256.566 kb on - strand, within RS_RS18150 at 256.566 kb on - strand, within RS_RS18150 at 256.580 kb on + strand, within RS_RS18150 at 256.581 kb on - strand, within RS_RS18150 at 256.581 kb on - strand, within RS_RS18150 at 256.581 kb on - strand, within RS_RS18150
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 254,070 + RS_RS18135 0.56 -1.1 254,127 + RS_RS18135 0.61 -0.5 254,201 + RS_RS18135 0.68 +0.2 254,202 - RS_RS18135 0.68 -0.8 254,239 + RS_RS18135 0.72 -1.5 254,239 + RS_RS18135 0.72 +0.1 254,239 + RS_RS18135 0.72 +0.6 254,239 + RS_RS18135 0.72 -1.4 254,239 + RS_RS18135 0.72 -0.3 254,239 + RS_RS18135 0.72 +2.2 254,239 + RS_RS18135 0.72 -1.1 254,239 + RS_RS18135 0.72 -0.6 254,240 - RS_RS18135 0.72 -0.3 254,240 - RS_RS18135 0.72 -0.1 254,240 - RS_RS18135 0.72 -0.4 254,240 - RS_RS18135 0.72 -1.9 254,240 - RS_RS18135 0.72 +0.1 254,240 - RS_RS18135 0.72 -0.9 254,240 - RS_RS18135 0.72 -0.2 254,240 - RS_RS18135 0.72 +2.2 254,240 - RS_RS18135 0.72 +0.1 254,392 + RS_RS18135 0.87 +0.4 254,497 + -1.0 254,498 - -0.2 254,499 + -1.1 254,499 + -0.3 254,499 + -0.2 254,499 + +0.3 254,500 - +0.1 254,500 - -1.0 255,709 + -0.0 255,733 + -0.3 255,734 - -0.2 255,734 - +0.6 255,734 - +0.8 255,735 + +0.2 255,735 + +1.4 255,735 + +0.8 255,736 - -0.7 255,751 - +1.2 255,801 - RS_RS18145 0.18 -0.9 255,801 - RS_RS18145 0.18 -0.4 255,823 + RS_RS18145 0.22 -0.4 255,823 + RS_RS18145 0.22 +0.0 255,825 + RS_RS18145 0.23 -0.1 255,855 + RS_RS18145 0.29 +0.6 255,856 - RS_RS18145 0.29 +0.8 255,897 + RS_RS18145 0.38 -0.1 255,898 - RS_RS18145 0.38 -0.6 255,953 + RS_RS18145 0.49 +0.6 256,066 + RS_RS18145 0.72 -0.6 256,067 - RS_RS18145 0.72 -1.2 256,067 - RS_RS18145 0.72 -0.1 256,079 - RS_RS18145 0.75 -0.5 256,079 - RS_RS18145 0.75 -1.1 256,109 + RS_RS18145 0.81 +0.2 256,110 - RS_RS18145 0.81 -0.2 256,110 - RS_RS18145 0.81 +1.2 256,110 - RS_RS18145 0.81 -1.4 256,117 - RS_RS18145 0.83 -0.1 256,234 + -0.4 256,234 + +0.6 256,234 + +0.6 256,234 + +0.3 256,234 + -1.4 256,235 - -0.1 256,235 - -0.1 256,235 - -0.5 256,235 - +0.6 256,244 + +0.3 256,244 + -0.2 256,244 + -0.7 256,277 + +0.1 256,277 + -0.2 256,277 + +0.1 256,355 + +1.4 256,556 + RS_RS18150 0.21 -0.1 256,565 + RS_RS18150 0.21 +2.2 256,565 + RS_RS18150 0.21 -0.4 256,565 + RS_RS18150 0.21 -0.7 256,566 - RS_RS18150 0.22 -1.2 256,566 - RS_RS18150 0.22 +0.0 256,580 + RS_RS18150 0.22 +2.2 256,581 - RS_RS18150 0.22 +0.1 256,581 - RS_RS18150 0.22 +0.5 256,581 - RS_RS18150 0.22 -0.3
Or see this region's nucleotide sequence