Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS12915 and RS_RS12920 overlap by 4 nucleotides RS_RS12920 and RS_RS12925 are separated by 30 nucleotides RS_RS12925 and RS_RS12930 overlap by 4 nucleotides RS_RS12930 and RS_RS12935 are separated by 12 nucleotides
RS_RS12915: RS_RS12915 - P-type conjugative transfer protein TrbG, at 2,783,192 to 2,784,196
_RS12915
RS_RS12920: RS_RS12920 - conjugal transfer protein TrbF, at 2,784,193 to 2,784,897
_RS12920
RS_RS12925: RS_RS12925 - P-type conjugative transfer protein TrbL, at 2,784,928 to 2,786,301
_RS12925
RS_RS12930: RS_RS12930 - hypothetical protein, at 2,786,298 to 2,786,624
_RS12930
RS_RS12935: RS_RS12935 - P-type conjugative transfer protein TrbJ, at 2,786,637 to 2,787,374
_RS12935
Position (kb)
2784
2785
2786
2787 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2784.174 kb on + strand at 2784.174 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.252 kb on - strand at 2784.252 kb on - strand at 2784.256 kb on - strand at 2784.256 kb on - strand at 2784.512 kb on + strand, within RS_RS12920 at 2784.512 kb on + strand, within RS_RS12920 at 2784.518 kb on + strand, within RS_RS12920 at 2784.519 kb on - strand, within RS_RS12920 at 2784.519 kb on - strand, within RS_RS12920 at 2784.686 kb on + strand, within RS_RS12920 at 2784.686 kb on + strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.839 kb on + strand at 2784.840 kb on - strand at 2784.840 kb on - strand at 2784.922 kb on - strand at 2785.808 kb on - strand, within RS_RS12925 at 2785.808 kb on - strand, within RS_RS12925 at 2786.014 kb on - strand, within RS_RS12925 at 2786.014 kb on - strand, within RS_RS12925 at 2786.075 kb on + strand, within RS_RS12925 at 2786.075 kb on + strand, within RS_RS12925 at 2786.094 kb on - strand, within RS_RS12925 at 2786.162 kb on + strand, within RS_RS12925 at 2786.193 kb on + strand at 2786.230 kb on + strand at 2786.230 kb on + strand at 2786.230 kb on + strand at 2786.252 kb on + strand at 2786.253 kb on - strand at 2786.265 kb on + strand at 2786.266 kb on - strand at 2786.647 kb on + strand at 2786.672 kb on - strand at 2786.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,784,174 + -0.1 2,784,174 + +0.9 2,784,251 + +0.4 2,784,251 + +0.3 2,784,251 + -0.3 2,784,251 + -0.9 2,784,251 + +0.3 2,784,252 - -0.7 2,784,252 - -2.1 2,784,256 - -4.0 2,784,256 - -3.4 2,784,512 + RS_RS12920 0.45 +0.1 2,784,512 + RS_RS12920 0.45 -0.0 2,784,518 + RS_RS12920 0.46 +5.0 2,784,519 - RS_RS12920 0.46 -1.2 2,784,519 - RS_RS12920 0.46 -4.2 2,784,686 + RS_RS12920 0.70 +1.3 2,784,686 + RS_RS12920 0.70 -0.5 2,784,687 - RS_RS12920 0.70 -2.6 2,784,687 - RS_RS12920 0.70 -3.5 2,784,687 - RS_RS12920 0.70 -2.1 2,784,839 + +0.1 2,784,840 - -3.4 2,784,840 - -3.6 2,784,922 - -1.9 2,785,808 - RS_RS12925 0.64 -2.3 2,785,808 - RS_RS12925 0.64 -4.1 2,786,014 - RS_RS12925 0.79 +0.5 2,786,014 - RS_RS12925 0.79 -4.7 2,786,075 + RS_RS12925 0.83 +0.7 2,786,075 + RS_RS12925 0.83 -0.8 2,786,094 - RS_RS12925 0.85 -2.5 2,786,162 + RS_RS12925 0.90 -0.5 2,786,193 + -0.1 2,786,230 + -0.1 2,786,230 + +0.2 2,786,230 + +0.3 2,786,252 + +2.3 2,786,253 - +1.3 2,786,265 + +2.3 2,786,266 - +0.9 2,786,647 + +0.5 2,786,672 - -1.3 2,786,672 - -1.5
Or see this region's nucleotide sequence