Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS12925

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS12915 and RS_RS12920 overlap by 4 nucleotidesRS_RS12920 and RS_RS12925 are separated by 30 nucleotidesRS_RS12925 and RS_RS12930 overlap by 4 nucleotidesRS_RS12930 and RS_RS12935 are separated by 12 nucleotides RS_RS12915: RS_RS12915 - P-type conjugative transfer protein TrbG, at 2,783,192 to 2,784,196 _RS12915 RS_RS12920: RS_RS12920 - conjugal transfer protein TrbF, at 2,784,193 to 2,784,897 _RS12920 RS_RS12925: RS_RS12925 - P-type conjugative transfer protein TrbL, at 2,784,928 to 2,786,301 _RS12925 RS_RS12930: RS_RS12930 - hypothetical protein, at 2,786,298 to 2,786,624 _RS12930 RS_RS12935: RS_RS12935 - P-type conjugative transfer protein TrbJ, at 2,786,637 to 2,787,374 _RS12935 Position (kb) 2784 2785 2786 2787Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 2784.174 kb on + strandat 2784.174 kb on + strandat 2784.251 kb on + strandat 2784.251 kb on + strandat 2784.251 kb on + strandat 2784.251 kb on + strandat 2784.251 kb on + strandat 2784.252 kb on - strandat 2784.252 kb on - strandat 2784.256 kb on - strandat 2784.256 kb on - strandat 2784.512 kb on + strand, within RS_RS12920at 2784.512 kb on + strand, within RS_RS12920at 2784.518 kb on + strand, within RS_RS12920at 2784.519 kb on - strand, within RS_RS12920at 2784.519 kb on - strand, within RS_RS12920at 2784.686 kb on + strand, within RS_RS12920at 2784.686 kb on + strand, within RS_RS12920at 2784.687 kb on - strand, within RS_RS12920at 2784.687 kb on - strand, within RS_RS12920at 2784.687 kb on - strand, within RS_RS12920at 2784.839 kb on + strandat 2784.840 kb on - strandat 2784.840 kb on - strandat 2784.922 kb on - strandat 2785.808 kb on - strand, within RS_RS12925at 2785.808 kb on - strand, within RS_RS12925at 2786.014 kb on - strand, within RS_RS12925at 2786.014 kb on - strand, within RS_RS12925at 2786.075 kb on + strand, within RS_RS12925at 2786.075 kb on + strand, within RS_RS12925at 2786.094 kb on - strand, within RS_RS12925at 2786.162 kb on + strand, within RS_RS12925at 2786.193 kb on + strandat 2786.230 kb on + strandat 2786.230 kb on + strandat 2786.230 kb on + strandat 2786.252 kb on + strandat 2786.253 kb on - strandat 2786.265 kb on + strandat 2786.266 kb on - strandat 2786.647 kb on + strandat 2786.672 kb on - strandat 2786.672 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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2,784,174 + -0.1
2,784,174 + +0.9
2,784,251 + +0.4
2,784,251 + +0.3
2,784,251 + -0.3
2,784,251 + -0.9
2,784,251 + +0.3
2,784,252 - -0.7
2,784,252 - -2.1
2,784,256 - -4.0
2,784,256 - -3.4
2,784,512 + RS_RS12920 0.45 +0.1
2,784,512 + RS_RS12920 0.45 -0.0
2,784,518 + RS_RS12920 0.46 +5.0
2,784,519 - RS_RS12920 0.46 -1.2
2,784,519 - RS_RS12920 0.46 -4.2
2,784,686 + RS_RS12920 0.70 +1.3
2,784,686 + RS_RS12920 0.70 -0.5
2,784,687 - RS_RS12920 0.70 -2.6
2,784,687 - RS_RS12920 0.70 -3.5
2,784,687 - RS_RS12920 0.70 -2.1
2,784,839 + +0.1
2,784,840 - -3.4
2,784,840 - -3.6
2,784,922 - -1.9
2,785,808 - RS_RS12925 0.64 -2.3
2,785,808 - RS_RS12925 0.64 -4.1
2,786,014 - RS_RS12925 0.79 +0.5
2,786,014 - RS_RS12925 0.79 -4.7
2,786,075 + RS_RS12925 0.83 +0.7
2,786,075 + RS_RS12925 0.83 -0.8
2,786,094 - RS_RS12925 0.85 -2.5
2,786,162 + RS_RS12925 0.90 -0.5
2,786,193 + -0.1
2,786,230 + -0.1
2,786,230 + +0.2
2,786,230 + +0.3
2,786,252 + +2.3
2,786,253 - +1.3
2,786,265 + +2.3
2,786,266 - +0.9
2,786,647 + +0.5
2,786,672 - -1.3
2,786,672 - -1.5

Or see this region's nucleotide sequence