Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS06270

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS06265 and RS_RS06270 are separated by 82 nucleotidesRS_RS06270 and RS_RS06275 are separated by 143 nucleotides RS_RS06265: RS_RS06265 - amino-acid N-acetyltransferase, at 1,325,532 to 1,326,929 _RS06265 RS_RS06270: RS_RS06270 - ATP-dependent RNA helicase HrpA, at 1,327,012 to 1,331,007 _RS06270 RS_RS06275: RS_RS06275 - YncE family protein, at 1,331,151 to 1,332,143 _RS06275 Position (kb) 1327 1328 1329 1330 1331 1332Strain fitness (log2 ratio) -1 0 1 2at 1327.472 kb on - strand, within RS_RS06270at 1327.778 kb on - strand, within RS_RS06270at 1327.778 kb on - strand, within RS_RS06270at 1328.137 kb on + strand, within RS_RS06270at 1328.860 kb on + strand, within RS_RS06270at 1328.861 kb on - strand, within RS_RS06270at 1329.412 kb on + strand, within RS_RS06270at 1329.605 kb on - strand, within RS_RS06270at 1329.605 kb on - strand, within RS_RS06270at 1329.658 kb on + strand, within RS_RS06270at 1329.787 kb on + strand, within RS_RS06270at 1330.362 kb on + strand, within RS_RS06270at 1330.362 kb on + strand, within RS_RS06270at 1330.363 kb on - strand, within RS_RS06270at 1331.150 kb on + strandat 1331.150 kb on + strandat 1331.150 kb on + strandat 1331.538 kb on + strand, within RS_RS06275

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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1,327,472 - RS_RS06270 0.12 -0.3
1,327,778 - RS_RS06270 0.19 -0.4
1,327,778 - RS_RS06270 0.19 -0.7
1,328,137 + RS_RS06270 0.28 +0.1
1,328,860 + RS_RS06270 0.46 -0.4
1,328,861 - RS_RS06270 0.46 +0.7
1,329,412 + RS_RS06270 0.60 +0.0
1,329,605 - RS_RS06270 0.65 +0.9
1,329,605 - RS_RS06270 0.65 +0.0
1,329,658 + RS_RS06270 0.66 +1.1
1,329,787 + RS_RS06270 0.69 +0.1
1,330,362 + RS_RS06270 0.84 +0.2
1,330,362 + RS_RS06270 0.84 +0.5
1,330,363 - RS_RS06270 0.84 +0.2
1,331,150 + +2.0
1,331,150 + +0.4
1,331,150 + +0.4
1,331,538 + RS_RS06275 0.39 -0.0

Or see this region's nucleotide sequence