Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS00845

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS00840 and RS_RS00845 are separated by 13 nucleotidesRS_RS00845 and RS_RS00850 overlap by 4 nucleotides RS_RS00840: RS_RS00840 - hypothetical protein, at 190,677 to 192,380 _RS00840 RS_RS00845: RS_RS00845 - short-chain dehydrogenase, at 192,394 to 193,134 _RS00845 RS_RS00850: RS_RS00850 - FAD-binding oxidoreductase, at 193,131 to 194,447 _RS00850 Position (kb) 192 193 194Strain fitness (log2 ratio) -2 -1 0 1 2at 191.493 kb on + strand, within RS_RS00840at 191.494 kb on - strand, within RS_RS00840at 191.494 kb on - strand, within RS_RS00840at 191.596 kb on + strand, within RS_RS00840at 191.596 kb on + strand, within RS_RS00840at 191.596 kb on + strand, within RS_RS00840at 191.596 kb on + strand, within RS_RS00840at 191.598 kb on + strand, within RS_RS00840at 191.599 kb on - strand, within RS_RS00840at 191.608 kb on + strand, within RS_RS00840at 191.608 kb on + strand, within RS_RS00840at 191.608 kb on + strand, within RS_RS00840at 191.608 kb on + strand, within RS_RS00840at 191.609 kb on - strand, within RS_RS00840at 191.689 kb on + strand, within RS_RS00840at 191.689 kb on + strand, within RS_RS00840at 191.689 kb on + strand, within RS_RS00840at 191.689 kb on + strand, within RS_RS00840at 191.690 kb on - strand, within RS_RS00840at 191.690 kb on - strand, within RS_RS00840at 191.690 kb on - strand, within RS_RS00840at 191.755 kb on + strand, within RS_RS00840at 191.755 kb on + strand, within RS_RS00840at 191.755 kb on + strand, within RS_RS00840at 191.755 kb on + strand, within RS_RS00840at 191.756 kb on - strand, within RS_RS00840at 191.756 kb on - strand, within RS_RS00840at 191.756 kb on - strand, within RS_RS00840at 191.902 kb on - strand, within RS_RS00840at 192.066 kb on + strand, within RS_RS00840at 192.102 kb on + strand, within RS_RS00840at 192.103 kb on - strand, within RS_RS00840at 192.120 kb on + strand, within RS_RS00840at 192.120 kb on + strand, within RS_RS00840at 192.121 kb on - strand, within RS_RS00840at 192.121 kb on - strand, within RS_RS00840at 192.145 kb on + strand, within RS_RS00840at 192.146 kb on - strand, within RS_RS00840at 192.146 kb on - strand, within RS_RS00840at 192.146 kb on - strand, within RS_RS00840at 192.150 kb on + strand, within RS_RS00840at 192.150 kb on + strand, within RS_RS00840at 192.150 kb on + strand, within RS_RS00840at 192.150 kb on + strand, within RS_RS00840at 192.151 kb on - strand, within RS_RS00840at 192.245 kb on - strandat 192.245 kb on - strandat 192.329 kb on + strandat 192.330 kb on - strandat 192.330 kb on - strandat 192.330 kb on - strandat 192.356 kb on - strandat 192.396 kb on - strandat 192.474 kb on - strand, within RS_RS00845at 192.474 kb on - strand, within RS_RS00845at 192.684 kb on - strand, within RS_RS00845at 192.690 kb on - strand, within RS_RS00845at 192.690 kb on - strand, within RS_RS00845at 193.573 kb on + strand, within RS_RS00850at 193.583 kb on - strand, within RS_RS00850at 193.583 kb on - strand, within RS_RS00850at 193.628 kb on - strand, within RS_RS00850at 193.628 kb on - strand, within RS_RS00850at 193.810 kb on + strand, within RS_RS00850at 193.810 kb on + strand, within RS_RS00850at 193.810 kb on + strand, within RS_RS00850at 194.123 kb on - strand, within RS_RS00850

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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191,493 + RS_RS00840 0.48 +0.8
191,494 - RS_RS00840 0.48 -0.4
191,494 - RS_RS00840 0.48 +0.7
191,596 + RS_RS00840 0.54 +0.2
191,596 + RS_RS00840 0.54 +2.0
191,596 + RS_RS00840 0.54 -0.3
191,596 + RS_RS00840 0.54 -0.5
191,598 + RS_RS00840 0.54 -0.4
191,599 - RS_RS00840 0.54 -0.7
191,608 + RS_RS00840 0.55 +0.1
191,608 + RS_RS00840 0.55 -0.6
191,608 + RS_RS00840 0.55 -0.4
191,608 + RS_RS00840 0.55 -1.1
191,609 - RS_RS00840 0.55 +1.4
191,689 + RS_RS00840 0.59 -0.5
191,689 + RS_RS00840 0.59 -0.8
191,689 + RS_RS00840 0.59 -1.3
191,689 + RS_RS00840 0.59 -1.0
191,690 - RS_RS00840 0.59 +0.1
191,690 - RS_RS00840 0.59 +0.5
191,690 - RS_RS00840 0.59 +0.7
191,755 + RS_RS00840 0.63 -0.9
191,755 + RS_RS00840 0.63 +2.4
191,755 + RS_RS00840 0.63 -1.2
191,755 + RS_RS00840 0.63 -0.4
191,756 - RS_RS00840 0.63 -0.8
191,756 - RS_RS00840 0.63 -0.3
191,756 - RS_RS00840 0.63 +0.8
191,902 - RS_RS00840 0.72 -0.6
192,066 + RS_RS00840 0.82 -1.9
192,102 + RS_RS00840 0.84 -0.3
192,103 - RS_RS00840 0.84 +0.1
192,120 + RS_RS00840 0.85 -0.5
192,120 + RS_RS00840 0.85 -0.4
192,121 - RS_RS00840 0.85 +0.4
192,121 - RS_RS00840 0.85 +0.5
192,145 + RS_RS00840 0.86 +1.4
192,146 - RS_RS00840 0.86 -0.5
192,146 - RS_RS00840 0.86 +0.2
192,146 - RS_RS00840 0.86 +0.5
192,150 + RS_RS00840 0.86 -0.3
192,150 + RS_RS00840 0.86 -0.3
192,150 + RS_RS00840 0.86 +0.2
192,150 + RS_RS00840 0.86 -0.1
192,151 - RS_RS00840 0.87 +1.1
192,245 - +0.2
192,245 - +0.4
192,329 + -0.7
192,330 - -0.1
192,330 - +0.2
192,330 - +1.0
192,356 - -1.7
192,396 - +0.2
192,474 - RS_RS00845 0.11 +0.1
192,474 - RS_RS00845 0.11 -0.2
192,684 - RS_RS00845 0.39 -1.1
192,690 - RS_RS00845 0.40 -1.3
192,690 - RS_RS00845 0.40 +0.8
193,573 + RS_RS00850 0.34 +0.4
193,583 - RS_RS00850 0.34 -0.2
193,583 - RS_RS00850 0.34 +1.4
193,628 - RS_RS00850 0.38 -1.2
193,628 - RS_RS00850 0.38 -1.2
193,810 + RS_RS00850 0.52 -0.6
193,810 + RS_RS00850 0.52 +1.4
193,810 + RS_RS00850 0.52 +0.8
194,123 - RS_RS00850 0.75 +1.4

Or see this region's nucleotide sequence