Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS12890

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS12885 and RS_RS12890 overlap by 1 nucleotidesRS_RS12890 and RS_RS12895 are separated by 39 nucleotidesRS_RS12895 and RS_RS12900 are separated by 109 nucleotides RS_RS12885: RS_RS12885 - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 2,778,248 to 2,779,312 _RS12885 RS_RS12890: RS_RS12890 - metal-binding protein, at 2,779,312 to 2,779,581 _RS12890 RS_RS12895: RS_RS12895 - hypothetical protein, at 2,779,621 to 2,780,043 _RS12895 RS_RS12900: RS_RS12900 - ISL3 family transposase ISRso15, at 2,780,153 to 2,781,373 _RS12900 Position (kb) 2779 2780Strain fitness (log2 ratio) -1 0 1at 2778.339 kb on - strandat 2778.362 kb on + strand, within RS_RS12885at 2778.449 kb on + strand, within RS_RS12885at 2778.719 kb on + strand, within RS_RS12885at 2778.752 kb on + strand, within RS_RS12885at 2778.752 kb on + strand, within RS_RS12885at 2778.753 kb on - strand, within RS_RS12885at 2778.753 kb on - strand, within RS_RS12885at 2779.408 kb on + strand, within RS_RS12890at 2779.409 kb on - strand, within RS_RS12890at 2779.459 kb on + strand, within RS_RS12890at 2779.828 kb on + strand, within RS_RS12895at 2779.829 kb on - strand, within RS_RS12895at 2780.116 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,778,339 - +1.7
2,778,362 + RS_RS12885 0.11 +0.1
2,778,449 + RS_RS12885 0.19 -0.2
2,778,719 + RS_RS12885 0.44 -1.7
2,778,752 + RS_RS12885 0.47 +0.2
2,778,752 + RS_RS12885 0.47 +0.1
2,778,753 - RS_RS12885 0.47 -0.4
2,778,753 - RS_RS12885 0.47 -0.2
2,779,408 + RS_RS12890 0.36 +1.7
2,779,409 - RS_RS12890 0.36 +0.2
2,779,459 + RS_RS12890 0.54 -0.4
2,779,828 + RS_RS12895 0.49 +0.4
2,779,829 - RS_RS12895 0.49 +1.2
2,780,116 - -0.1

Or see this region's nucleotide sequence