Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00785 and RS_RS00790 are separated by 140 nucleotides RS_RS00790 and RS_RS00795 overlap by 4 nucleotides
RS_RS00785: RS_RS00785 - ornithine--oxo-acid transaminase, at 178,653 to 179,879
_RS00785
RS_RS00790: RS_RS00790 - LysR family transcriptional regulator, at 180,020 to 180,946
_RS00790
RS_RS00795: RS_RS00795 - aldehyde dehydrogenase family protein, at 180,943 to 182,454
_RS00795
Position (kb) 180
181 Strain fitness (log2 ratio) -1
0
1
2 at 179.404 kb on + strand, within RS_RS00785 at 179.405 kb on - strand, within RS_RS00785 at 179.405 kb on - strand, within RS_RS00785 at 179.405 kb on - strand, within RS_RS00785 at 179.749 kb on + strand, within RS_RS00785 at 179.819 kb on - strand at 179.852 kb on - strand at 179.852 kb on - strand at 180.042 kb on - strand at 180.050 kb on - strand at 180.128 kb on + strand, within RS_RS00790 at 180.298 kb on - strand, within RS_RS00790 at 180.521 kb on + strand, within RS_RS00790 at 180.521 kb on + strand, within RS_RS00790 at 180.521 kb on + strand, within RS_RS00790 at 180.620 kb on + strand, within RS_RS00790 at 180.621 kb on - strand, within RS_RS00790 at 180.641 kb on + strand, within RS_RS00790 at 180.641 kb on + strand, within RS_RS00790 at 180.642 kb on - strand, within RS_RS00790 at 180.944 kb on + strand at 180.944 kb on + strand at 180.945 kb on - strand at 180.996 kb on - strand at 180.996 kb on - strand at 181.025 kb on + strand at 181.025 kb on + strand at 181.025 kb on + strand at 181.026 kb on - strand at 181.271 kb on + strand, within RS_RS00795 at 181.271 kb on + strand, within RS_RS00795 at 181.271 kb on + strand, within RS_RS00795 at 181.271 kb on + strand, within RS_RS00795 at 181.272 kb on - strand, within RS_RS00795 at 181.272 kb on - strand, within RS_RS00795 at 181.272 kb on - strand, within RS_RS00795 at 181.272 kb on - strand, within RS_RS00795 at 181.346 kb on + strand, within RS_RS00795 at 181.346 kb on + strand, within RS_RS00795 at 181.347 kb on - strand, within RS_RS00795 at 181.347 kb on - strand, within RS_RS00795 at 181.347 kb on - strand, within RS_RS00795 at 181.350 kb on - strand, within RS_RS00795 at 181.350 kb on - strand, within RS_RS00795 at 181.350 kb on - strand, within RS_RS00795 at 181.446 kb on - strand, within RS_RS00795 at 181.550 kb on + strand, within RS_RS00795
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 179,404 + RS_RS00785 0.61 +0.3 179,405 - RS_RS00785 0.61 +0.0 179,405 - RS_RS00785 0.61 -0.0 179,405 - RS_RS00785 0.61 +1.9 179,749 + RS_RS00785 0.89 +0.4 179,819 - -1.1 179,852 - -0.1 179,852 - +1.9 180,042 - +0.0 180,050 - +0.3 180,128 + RS_RS00790 0.12 +0.0 180,298 - RS_RS00790 0.30 +0.9 180,521 + RS_RS00790 0.54 +0.5 180,521 + RS_RS00790 0.54 -0.1 180,521 + RS_RS00790 0.54 +0.1 180,620 + RS_RS00790 0.65 -0.1 180,621 - RS_RS00790 0.65 -0.2 180,641 + RS_RS00790 0.67 +1.0 180,641 + RS_RS00790 0.67 -0.3 180,642 - RS_RS00790 0.67 -0.1 180,944 + +0.8 180,944 + +0.2 180,945 - +0.2 180,996 - +0.2 180,996 - -0.1 181,025 + -0.1 181,025 + +0.1 181,025 + +1.9 181,026 - +0.2 181,271 + RS_RS00795 0.22 +0.4 181,271 + RS_RS00795 0.22 -0.6 181,271 + RS_RS00795 0.22 +0.4 181,271 + RS_RS00795 0.22 -0.5 181,272 - RS_RS00795 0.22 -0.0 181,272 - RS_RS00795 0.22 -0.2 181,272 - RS_RS00795 0.22 +0.9 181,272 - RS_RS00795 0.22 +0.5 181,346 + RS_RS00795 0.27 -0.3 181,346 + RS_RS00795 0.27 +0.4 181,347 - RS_RS00795 0.27 -0.3 181,347 - RS_RS00795 0.27 +1.9 181,347 - RS_RS00795 0.27 -1.0 181,350 - RS_RS00795 0.27 -0.1 181,350 - RS_RS00795 0.27 +0.1 181,350 - RS_RS00795 0.27 +0.5 181,446 - RS_RS00795 0.33 +0.0 181,550 + RS_RS00795 0.40 -0.2
Or see this region's nucleotide sequence