Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS09175

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRS_RS09170 and RS_RS09175 overlap by 4 nucleotidesRS_RS09175 and RS_RS09180 are separated by 473 nucleotides RS_RS09170: RS_RS09170 - methionine--tRNA ligase, at 1,987,271 to 1,988,761 _RS09170 RS_RS09175: RS_RS09175 - cupin domain-containing protein, at 1,988,758 to 1,989,102 _RS09175 RS_RS09180: RS_RS09180 - flavin reductase family protein, at 1,989,576 to 1,990,199 _RS09180 Position (kb) 1988 1989 1990Strain fitness (log2 ratio) -2 -1 0 1 2at 1988.006 kb on - strand, within RS_RS09170at 1988.058 kb on + strand, within RS_RS09170at 1988.058 kb on + strand, within RS_RS09170at 1988.059 kb on - strand, within RS_RS09170at 1988.059 kb on - strand, within RS_RS09170at 1988.165 kb on - strand, within RS_RS09170at 1988.252 kb on + strand, within RS_RS09170at 1988.351 kb on + strand, within RS_RS09170at 1988.351 kb on + strand, within RS_RS09170at 1988.498 kb on + strand, within RS_RS09170at 1988.727 kb on - strandat 1988.730 kb on + strandat 1988.731 kb on - strandat 1989.042 kb on + strand, within RS_RS09175at 1989.242 kb on + strandat 1989.243 kb on - strandat 1989.404 kb on - strandat 1989.404 kb on - strandat 1989.529 kb on + strandat 1989.717 kb on + strand, within RS_RS09180at 1989.741 kb on + strand, within RS_RS09180at 1989.742 kb on - strand, within RS_RS09180at 1990.032 kb on - strand, within RS_RS09180at 1990.098 kb on + strand, within RS_RS09180

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
remove
1,988,006 - RS_RS09170 0.49 +0.5
1,988,058 + RS_RS09170 0.53 -1.8
1,988,058 + RS_RS09170 0.53 -0.5
1,988,059 - RS_RS09170 0.53 -0.7
1,988,059 - RS_RS09170 0.53 -0.3
1,988,165 - RS_RS09170 0.60 +1.8
1,988,252 + RS_RS09170 0.66 -0.2
1,988,351 + RS_RS09170 0.72 -0.2
1,988,351 + RS_RS09170 0.72 +0.0
1,988,498 + RS_RS09170 0.82 +0.3
1,988,727 - +0.1
1,988,730 + +0.6
1,988,731 - +0.0
1,989,042 + RS_RS09175 0.82 +0.3
1,989,242 + +0.5
1,989,243 - +0.8
1,989,404 - -1.1
1,989,404 - -0.3
1,989,529 + -0.6
1,989,717 + RS_RS09180 0.23 +0.5
1,989,741 + RS_RS09180 0.26 -0.4
1,989,742 - RS_RS09180 0.27 +0.2
1,990,032 - RS_RS09180 0.73 +0.6
1,990,098 + RS_RS09180 0.84 -0.0

Or see this region's nucleotide sequence