Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS07995 and RS_RS08000 are separated by 203 nucleotides RS_RS08000 and RS_RS08005 are separated by 12 nucleotides
RS_RS07995: RS_RS07995 - DNA polymerase IV, at 1,702,478 to 1,703,566
_RS07995
RS_RS08000: RS_RS08000 - amino acid permease, at 1,703,770 to 1,705,200
_RS08000
RS_RS08005: RS_RS08005 - DUF1223 domain-containing protein, at 1,705,213 to 1,706,016
_RS08005
Position (kb) 1703
1704
1705
1706 Strain fitness (log2 ratio) -1
0
1 at 1702.770 kb on - strand, within RS_RS07995 at 1702.808 kb on + strand, within RS_RS07995 at 1703.237 kb on + strand, within RS_RS07995 at 1703.237 kb on + strand, within RS_RS07995 at 1703.384 kb on - strand, within RS_RS07995 at 1704.041 kb on - strand, within RS_RS08000 at 1704.041 kb on - strand, within RS_RS08000 at 1704.042 kb on + strand, within RS_RS08000 at 1704.042 kb on + strand, within RS_RS08000 at 1704.042 kb on + strand, within RS_RS08000 at 1704.043 kb on - strand, within RS_RS08000 at 1704.043 kb on - strand, within RS_RS08000 at 1704.043 kb on - strand, within RS_RS08000 at 1704.046 kb on + strand, within RS_RS08000 at 1704.046 kb on + strand, within RS_RS08000 at 1704.046 kb on + strand, within RS_RS08000 at 1704.047 kb on - strand, within RS_RS08000 at 1704.052 kb on + strand, within RS_RS08000 at 1704.151 kb on + strand, within RS_RS08000 at 1704.571 kb on + strand, within RS_RS08000 at 1704.838 kb on - strand, within RS_RS08000 at 1704.909 kb on + strand, within RS_RS08000 at 1704.910 kb on - strand, within RS_RS08000 at 1705.270 kb on + strand at 1705.270 kb on + strand at 1705.270 kb on + strand at 1705.504 kb on + strand, within RS_RS08005 at 1705.535 kb on - strand, within RS_RS08005 at 1705.679 kb on + strand, within RS_RS08005 at 1705.680 kb on - strand, within RS_RS08005 at 1705.776 kb on - strand, within RS_RS08005 at 1705.877 kb on + strand, within RS_RS08005 at 1705.878 kb on - strand, within RS_RS08005 at 1705.900 kb on - strand, within RS_RS08005 at 1705.997 kb on - strand at 1705.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,702,770 - RS_RS07995 0.27 +0.2 1,702,808 + RS_RS07995 0.30 +0.1 1,703,237 + RS_RS07995 0.70 +0.5 1,703,237 + RS_RS07995 0.70 +0.1 1,703,384 - RS_RS07995 0.83 +0.6 1,704,041 - RS_RS08000 0.19 -0.1 1,704,041 - RS_RS08000 0.19 -1.1 1,704,042 + RS_RS08000 0.19 -0.0 1,704,042 + RS_RS08000 0.19 -0.7 1,704,042 + RS_RS08000 0.19 +0.1 1,704,043 - RS_RS08000 0.19 -0.4 1,704,043 - RS_RS08000 0.19 -1.4 1,704,043 - RS_RS08000 0.19 -0.3 1,704,046 + RS_RS08000 0.19 -0.6 1,704,046 + RS_RS08000 0.19 -0.7 1,704,046 + RS_RS08000 0.19 -0.1 1,704,047 - RS_RS08000 0.19 -1.1 1,704,052 + RS_RS08000 0.20 +0.6 1,704,151 + RS_RS08000 0.27 -0.8 1,704,571 + RS_RS08000 0.56 -0.9 1,704,838 - RS_RS08000 0.75 +0.5 1,704,909 + RS_RS08000 0.80 -0.4 1,704,910 - RS_RS08000 0.80 -0.4 1,705,270 + -0.2 1,705,270 + -0.6 1,705,270 + -0.1 1,705,504 + RS_RS08005 0.36 -0.5 1,705,535 - RS_RS08005 0.40 +0.3 1,705,679 + RS_RS08005 0.58 -0.1 1,705,680 - RS_RS08005 0.58 -0.1 1,705,776 - RS_RS08005 0.70 +0.0 1,705,877 + RS_RS08005 0.83 +0.0 1,705,878 - RS_RS08005 0.83 -0.0 1,705,900 - RS_RS08005 0.85 +0.1 1,705,997 - +0.7 1,705,997 - +1.0
Or see this region's nucleotide sequence