Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS06075

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS06070 and RS_RS06075 overlap by 4 nucleotidesRS_RS06075 and RS_RS06080 overlap by 8 nucleotides RS_RS06070: RS_RS06070 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 1,280,644 to 1,281,795 _RS06070 RS_RS06075: RS_RS06075 - type IV pilus biogenesis/stability protein PilW, at 1,281,792 to 1,282,583 _RS06075 RS_RS06080: RS_RS06080 - DUF4115 domain-containing protein, at 1,282,576 to 1,283,679 _RS06080 Position (kb) 1281 1282 1283Strain fitness (log2 ratio) -1 0 1at 1281.169 kb on + strand, within RS_RS06070at 1281.169 kb on + strand, within RS_RS06070at 1282.320 kb on + strand, within RS_RS06075at 1283.493 kb on + strand, within RS_RS06080

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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1,281,169 + RS_RS06070 0.46 +0.8
1,281,169 + RS_RS06070 0.46 -1.0
1,282,320 + RS_RS06075 0.67 +0.2
1,283,493 + RS_RS06080 0.83 -0.3

Or see this region's nucleotide sequence