Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00660 and RS_RS00665 are separated by 240 nucleotides RS_RS00665 and RS_RS00670 are separated by 17 nucleotides RS_RS00670 and RS_RS00675 are separated by 78 nucleotides
RS_RS00660: RS_RS00660 - S-adenosylmethionine synthase, at 156,370 to 157,560
_RS00660
RS_RS00665: RS_RS00665 - lauroyl acyltransferase, at 157,801 to 158,649
_RS00665
RS_RS00670: RS_RS00670 - lauroyl acyltransferase, at 158,667 to 159,560
_RS00670
RS_RS00675: RS_RS00675 - diaminopimelate epimerase, at 159,639 to 160,517
_RS00675
Position (kb) 157
158
159 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 157.635 kb on - strand at 157.635 kb on - strand at 157.635 kb on - strand at 157.713 kb on + strand at 157.713 kb on + strand at 157.713 kb on + strand at 158.087 kb on + strand, within RS_RS00665 at 158.088 kb on - strand, within RS_RS00665 at 158.155 kb on + strand, within RS_RS00665 at 158.318 kb on + strand, within RS_RS00665 at 158.390 kb on - strand, within RS_RS00665 at 158.391 kb on + strand, within RS_RS00665 at 158.391 kb on + strand, within RS_RS00665 at 158.392 kb on - strand, within RS_RS00665 at 158.392 kb on - strand, within RS_RS00665 at 158.486 kb on + strand, within RS_RS00665 at 158.486 kb on + strand, within RS_RS00665 at 158.587 kb on + strand at 158.587 kb on + strand at 158.587 kb on + strand at 158.587 kb on + strand at 158.599 kb on + strand at 158.599 kb on + strand at 158.601 kb on + strand at 158.608 kb on + strand at 158.608 kb on + strand at 158.666 kb on + strand at 159.555 kb on + strand at 159.556 kb on - strand at 159.556 kb on - strand at 159.556 kb on - strand at 159.556 kb on - strand at 159.590 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 157,635 - -0.2 157,635 - +0.2 157,635 - +0.4 157,713 + +0.3 157,713 + -0.1 157,713 + -0.3 158,087 + RS_RS00665 0.34 -4.5 158,088 - RS_RS00665 0.34 -4.3 158,155 + RS_RS00665 0.42 -3.4 158,318 + RS_RS00665 0.61 -4.9 158,390 - RS_RS00665 0.69 -4.4 158,391 + RS_RS00665 0.69 -3.6 158,391 + RS_RS00665 0.69 -4.6 158,392 - RS_RS00665 0.70 -5.3 158,392 - RS_RS00665 0.70 -4.4 158,486 + RS_RS00665 0.81 -4.6 158,486 + RS_RS00665 0.81 -4.9 158,587 + -4.1 158,587 + -4.2 158,587 + -5.2 158,587 + -4.4 158,599 + -5.9 158,599 + -5.6 158,601 + -3.9 158,608 + -4.5 158,608 + -4.4 158,666 + +0.0 159,555 + -0.3 159,556 - -1.8 159,556 - -1.2 159,556 - -1.5 159,556 - -1.2 159,590 + -0.2
Or see this region's nucleotide sequence