Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS19240 and RS_RS19245 are separated by 145 nucleotides RS_RS19245 and RS_RS19250 are separated by 313 nucleotides RS_RS19250 and RS_RS26075 overlap by 44 nucleotides RS_RS26075 and RS_RS19255 are separated by 221 nucleotides
RS_RS19240: RS_RS19240 - hypothetical protein, at 546,032 to 546,712
_RS19240
RS_RS19245: RS_RS19245 - hypothetical protein, at 546,858 to 547,139
_RS19245
RS_RS19250: RS_RS19250 - transcriptional regulator GcvA, at 547,453 to 548,367
_RS19250
RS_RS26075: RS_RS26075 - hypothetical protein, at 548,324 to 548,536
_RS26075
RS_RS19255: RS_RS19255 - GntR family transcriptional regulator, at 548,758 to 549,414
_RS19255
Position (kb) 547
548
549 Strain fitness (log2 ratio) -1
0
1
2 at 546.459 kb on + strand, within RS_RS19240 at 546.491 kb on + strand, within RS_RS19240 at 546.491 kb on + strand, within RS_RS19240 at 546.491 kb on + strand, within RS_RS19240 at 546.491 kb on + strand, within RS_RS19240 at 546.491 kb on + strand, within RS_RS19240 at 546.492 kb on - strand, within RS_RS19240 at 546.568 kb on - strand, within RS_RS19240 at 546.660 kb on + strand at 546.661 kb on - strand at 546.661 kb on - strand at 546.733 kb on + strand at 546.811 kb on - strand at 546.871 kb on + strand at 546.872 kb on - strand at 546.944 kb on + strand, within RS_RS19245 at 546.948 kb on + strand, within RS_RS19245 at 546.948 kb on + strand, within RS_RS19245 at 546.968 kb on - strand, within RS_RS19245 at 546.991 kb on - strand, within RS_RS19245 at 546.996 kb on + strand, within RS_RS19245 at 546.996 kb on + strand, within RS_RS19245 at 546.996 kb on + strand, within RS_RS19245 at 546.996 kb on + strand, within RS_RS19245 at 546.997 kb on - strand, within RS_RS19245 at 547.066 kb on + strand, within RS_RS19245 at 547.066 kb on + strand, within RS_RS19245 at 547.066 kb on + strand, within RS_RS19245 at 547.067 kb on - strand, within RS_RS19245 at 547.067 kb on - strand, within RS_RS19245 at 547.075 kb on + strand, within RS_RS19245 at 547.133 kb on - strand at 547.133 kb on - strand at 547.151 kb on + strand at 547.151 kb on + strand at 547.151 kb on + strand at 547.151 kb on + strand at 547.152 kb on - strand at 547.152 kb on - strand at 547.152 kb on - strand at 547.155 kb on + strand at 547.155 kb on + strand at 547.156 kb on - strand at 547.156 kb on - strand at 547.469 kb on - strand at 547.677 kb on - strand, within RS_RS19250 at 547.898 kb on - strand, within RS_RS19250 at 547.954 kb on + strand, within RS_RS19250 at 547.954 kb on + strand, within RS_RS19250 at 547.954 kb on + strand, within RS_RS19250 at 547.955 kb on - strand, within RS_RS19250 at 547.955 kb on - strand, within RS_RS19250 at 547.955 kb on - strand, within RS_RS19250 at 547.976 kb on + strand, within RS_RS19250 at 547.976 kb on + strand, within RS_RS19250 at 547.976 kb on + strand, within RS_RS19250 at 547.977 kb on - strand, within RS_RS19250 at 547.977 kb on - strand, within RS_RS19250 at 548.278 kb on + strand at 548.279 kb on - strand at 548.279 kb on - strand at 548.281 kb on + strand at 548.281 kb on + strand at 548.281 kb on + strand at 548.282 kb on - strand at 548.282 kb on - strand at 548.282 kb on - strand at 548.282 kb on - strand at 548.291 kb on - strand at 548.291 kb on - strand at 548.429 kb on - strand, within RS_RS26075 at 548.465 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.525 kb on + strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.526 kb on - strand at 548.529 kb on + strand at 548.529 kb on + strand at 548.529 kb on + strand at 548.529 kb on + strand at 548.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 546,459 + RS_RS19240 0.63 -0.3 546,491 + RS_RS19240 0.67 +1.2 546,491 + RS_RS19240 0.67 -0.2 546,491 + RS_RS19240 0.67 +0.2 546,491 + RS_RS19240 0.67 -1.0 546,491 + RS_RS19240 0.67 +0.2 546,492 - RS_RS19240 0.68 +0.2 546,568 - RS_RS19240 0.79 +0.5 546,660 + +0.0 546,661 - -0.3 546,661 - +0.0 546,733 + -0.1 546,811 - -0.2 546,871 + -0.8 546,872 - -0.3 546,944 + RS_RS19245 0.30 +0.1 546,948 + RS_RS19245 0.32 -0.4 546,948 + RS_RS19245 0.32 -0.6 546,968 - RS_RS19245 0.39 -0.3 546,991 - RS_RS19245 0.47 +0.1 546,996 + RS_RS19245 0.49 -0.2 546,996 + RS_RS19245 0.49 -1.2 546,996 + RS_RS19245 0.49 -0.1 546,996 + RS_RS19245 0.49 -0.6 546,997 - RS_RS19245 0.49 +0.0 547,066 + RS_RS19245 0.74 +0.5 547,066 + RS_RS19245 0.74 +0.1 547,066 + RS_RS19245 0.74 -0.3 547,067 - RS_RS19245 0.74 -1.0 547,067 - RS_RS19245 0.74 -0.6 547,075 + RS_RS19245 0.77 -0.1 547,133 - -0.6 547,133 - -0.8 547,151 + +0.0 547,151 + +0.1 547,151 + -0.5 547,151 + -0.8 547,152 - -0.7 547,152 - -0.2 547,152 - -0.4 547,155 + +0.1 547,155 + +0.3 547,156 - +0.2 547,156 - -0.5 547,469 - -0.2 547,677 - RS_RS19250 0.24 +0.1 547,898 - RS_RS19250 0.49 +1.2 547,954 + RS_RS19250 0.55 -0.8 547,954 + RS_RS19250 0.55 +0.2 547,954 + RS_RS19250 0.55 -0.3 547,955 - RS_RS19250 0.55 -0.5 547,955 - RS_RS19250 0.55 -1.3 547,955 - RS_RS19250 0.55 +0.8 547,976 + RS_RS19250 0.57 -0.1 547,976 + RS_RS19250 0.57 -0.1 547,976 + RS_RS19250 0.57 +0.2 547,977 - RS_RS19250 0.57 -0.3 547,977 - RS_RS19250 0.57 +1.4 548,278 + +0.6 548,279 - +0.3 548,279 - -0.1 548,281 + -0.3 548,281 + -0.2 548,281 + +0.3 548,282 - -0.4 548,282 - -0.0 548,282 - +0.1 548,282 - +0.8 548,291 - +0.2 548,291 - +0.2 548,429 - RS_RS26075 0.49 +1.2 548,465 + +0.8 548,525 + +0.7 548,525 + -0.2 548,525 + +2.0 548,525 + -0.6 548,525 + +0.7 548,525 + -0.1 548,525 + -0.2 548,525 + -0.2 548,526 - +0.4 548,526 - +0.4 548,526 - -0.1 548,526 - -0.3 548,526 - -0.3 548,526 - -0.2 548,526 - +0.1 548,526 - -0.2 548,526 - +1.2 548,529 + -1.1 548,529 + +0.4 548,529 + -0.1 548,529 + +0.2 548,530 - -1.6
Or see this region's nucleotide sequence