Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16855 and RS_RS16860 are separated by 46 nucleotides RS_RS16860 and RS_RS16865 are separated by 9 nucleotides RS_RS16865 and RS_RS16870 are separated by 120 nucleotides
RS_RS16855: RS_RS16855 - septum site-determining protein MinC, at 3,624,216 to 3,624,983
_RS16855
RS_RS16860: RS_RS16860 - septum site-determining protein MinD, at 3,625,030 to 3,625,845
_RS16860
RS_RS16865: RS_RS16865 - cell division topological specificity factor, at 3,625,855 to 3,626,109
_RS16865
RS_RS16870: RS_RS16870 - CoA transferase, at 3,626,230 to 3,627,678
_RS16870
Position (kb)
3625
3626
3627 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3624.879 kb on + strand, within RS_RS16855 at 3624.879 kb on + strand, within RS_RS16855 at 3624.880 kb on - strand, within RS_RS16855 at 3624.880 kb on - strand, within RS_RS16855 at 3625.010 kb on - strand at 3625.020 kb on + strand at 3625.020 kb on + strand at 3625.021 kb on - strand at 3625.046 kb on + strand at 3625.046 kb on + strand at 3625.046 kb on + strand at 3625.047 kb on - strand at 3625.201 kb on + strand, within RS_RS16860 at 3625.201 kb on + strand, within RS_RS16860 at 3625.201 kb on + strand, within RS_RS16860 at 3625.202 kb on - strand, within RS_RS16860 at 3625.375 kb on + strand, within RS_RS16860 at 3625.375 kb on + strand, within RS_RS16860 at 3625.376 kb on - strand, within RS_RS16860 at 3625.376 kb on - strand, within RS_RS16860 at 3625.376 kb on - strand, within RS_RS16860 at 3625.376 kb on - strand, within RS_RS16860 at 3625.376 kb on - strand, within RS_RS16860 at 3625.430 kb on - strand, within RS_RS16860 at 3625.430 kb on - strand, within RS_RS16860 at 3625.430 kb on - strand, within RS_RS16860 at 3625.576 kb on + strand, within RS_RS16860 at 3625.576 kb on + strand, within RS_RS16860 at 3625.576 kb on + strand, within RS_RS16860 at 3625.577 kb on - strand, within RS_RS16860 at 3625.745 kb on - strand, within RS_RS16860 at 3625.801 kb on + strand at 3625.801 kb on + strand at 3625.801 kb on + strand at 3625.801 kb on + strand at 3625.802 kb on - strand at 3625.843 kb on + strand at 3625.843 kb on + strand at 3626.197 kb on + strand at 3626.197 kb on + strand at 3626.197 kb on + strand at 3626.197 kb on + strand at 3626.198 kb on - strand at 3626.451 kb on + strand, within RS_RS16870 at 3626.584 kb on + strand, within RS_RS16870
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 3,624,879 + RS_RS16855 0.86 +0.1 3,624,879 + RS_RS16855 0.86 -0.7 3,624,880 - RS_RS16855 0.86 +0.1 3,624,880 - RS_RS16855 0.86 -2.9 3,625,010 - -0.7 3,625,020 + -0.3 3,625,020 + -0.4 3,625,021 - +1.7 3,625,046 + -0.5 3,625,046 + +0.1 3,625,046 + -1.2 3,625,047 - -0.2 3,625,201 + RS_RS16860 0.21 -0.3 3,625,201 + RS_RS16860 0.21 -0.5 3,625,201 + RS_RS16860 0.21 -0.5 3,625,202 - RS_RS16860 0.21 -0.2 3,625,375 + RS_RS16860 0.42 +1.7 3,625,375 + RS_RS16860 0.42 -0.3 3,625,376 - RS_RS16860 0.42 +1.7 3,625,376 - RS_RS16860 0.42 -0.7 3,625,376 - RS_RS16860 0.42 +0.7 3,625,376 - RS_RS16860 0.42 -1.2 3,625,376 - RS_RS16860 0.42 -0.6 3,625,430 - RS_RS16860 0.49 +0.7 3,625,430 - RS_RS16860 0.49 -0.7 3,625,430 - RS_RS16860 0.49 -0.6 3,625,576 + RS_RS16860 0.67 -0.3 3,625,576 + RS_RS16860 0.67 -0.3 3,625,576 + RS_RS16860 0.67 +0.7 3,625,577 - RS_RS16860 0.67 +1.7 3,625,745 - RS_RS16860 0.88 +0.4 3,625,801 + +0.2 3,625,801 + -0.7 3,625,801 + +0.7 3,625,801 + -0.8 3,625,802 - -1.0 3,625,843 + +1.7 3,625,843 + -0.1 3,626,197 + +0.1 3,626,197 + +1.7 3,626,197 + +0.1 3,626,197 + -0.0 3,626,198 - +1.3 3,626,451 + RS_RS16870 0.15 -0.3 3,626,584 + RS_RS16870 0.24 -0.1
Or see this region's nucleotide sequence