Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS06080

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS06070 and RS_RS06075 overlap by 4 nucleotidesRS_RS06075 and RS_RS06080 overlap by 8 nucleotidesRS_RS06080 and RS_RS06085 are separated by 54 nucleotides RS_RS06070: RS_RS06070 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 1,280,644 to 1,281,795 _RS06070 RS_RS06075: RS_RS06075 - type IV pilus biogenesis/stability protein PilW, at 1,281,792 to 1,282,583 _RS06075 RS_RS06080: RS_RS06080 - DUF4115 domain-containing protein, at 1,282,576 to 1,283,679 _RS06080 RS_RS06085: RS_RS06085 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin), at 1,283,734 to 1,285,029 _RS06085 Position (kb) 1282 1283 1284Strain fitness (log2 ratio) -1 0 1at 1282.320 kb on + strand, within RS_RS06075at 1283.493 kb on + strand, within RS_RS06080at 1283.641 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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1,282,320 + RS_RS06075 0.67 +1.2
1,283,493 + RS_RS06080 0.83 +0.0
1,283,641 + +0.7

Or see this region's nucleotide sequence