Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01390 and RS_RS01395 overlap by 1 nucleotides RS_RS01395 and RS_RS01400 are separated by 116 nucleotides RS_RS01400 and RS_RS01405 are separated by 101 nucleotides RS_RS01405 and RS_RS01410 are separated by 61 nucleotides
RS_RS01390: RS_RS01390 - hypothetical protein, at 315,826 to 316,671
_RS01390
RS_RS01395: RS_RS01395 - short-chain dehydrogenase, at 316,671 to 317,471
_RS01395
RS_RS01400: RS_RS01400 - thiol:disulfide interchange protein DsbA/DsbL, at 317,588 to 318,244
_RS01400
RS_RS01405: RS_RS01405 - SPOR domain-containing protein, at 318,346 to 319,062
_RS01405
RS_RS01410: RS_RS01410 - arginine--tRNA ligase, at 319,124 to 320,926
_RS01410
Position (kb) 317
318
319 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 316.727 kb on - strand at 317.006 kb on - strand, within RS_RS01395 at 317.006 kb on - strand, within RS_RS01395 at 317.308 kb on + strand, within RS_RS01395 at 317.308 kb on + strand, within RS_RS01395 at 317.309 kb on - strand, within RS_RS01395 at 317.309 kb on - strand, within RS_RS01395 at 317.309 kb on - strand, within RS_RS01395 at 317.623 kb on - strand at 317.623 kb on - strand at 317.623 kb on - strand at 317.679 kb on + strand, within RS_RS01400 at 317.721 kb on + strand, within RS_RS01400 at 317.722 kb on - strand, within RS_RS01400 at 317.722 kb on - strand, within RS_RS01400 at 317.722 kb on - strand, within RS_RS01400 at 317.753 kb on - strand, within RS_RS01400 at 317.950 kb on - strand, within RS_RS01400 at 317.953 kb on - strand, within RS_RS01400 at 318.456 kb on - strand, within RS_RS01405 at 318.576 kb on - strand, within RS_RS01405 at 319.074 kb on + strand at 319.074 kb on + strand at 319.075 kb on - strand at 319.092 kb on + strand at 319.092 kb on + strand at 319.092 kb on + strand at 319.093 kb on - strand at 319.093 kb on - strand at 319.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 316,727 - -0.6 317,006 - RS_RS01395 0.42 -0.7 317,006 - RS_RS01395 0.42 +0.3 317,308 + RS_RS01395 0.80 -0.1 317,308 + RS_RS01395 0.80 +0.3 317,309 - RS_RS01395 0.80 +0.1 317,309 - RS_RS01395 0.80 +0.1 317,309 - RS_RS01395 0.80 -0.4 317,623 - -3.3 317,623 - -0.7 317,623 - -2.2 317,679 + RS_RS01400 0.14 -2.3 317,721 + RS_RS01400 0.20 -2.2 317,722 - RS_RS01400 0.20 -2.0 317,722 - RS_RS01400 0.20 -1.7 317,722 - RS_RS01400 0.20 -1.2 317,753 - RS_RS01400 0.25 -3.1 317,950 - RS_RS01400 0.55 -0.0 317,953 - RS_RS01400 0.56 -0.4 318,456 - RS_RS01405 0.15 +0.6 318,576 - RS_RS01405 0.32 -0.4 319,074 + +2.6 319,074 + -1.1 319,075 - +0.6 319,092 + +1.2 319,092 + +0.6 319,092 + -0.7 319,093 - +1.6 319,093 - +1.6 319,093 - -0.4
Or see this region's nucleotide sequence