Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14285

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14280 and RS_RS14285 are separated by 234 nucleotidesRS_RS14285 and RS_RS14290 are separated by 82 nucleotides RS_RS14280: RS_RS14280 - porin, at 3,072,456 to 3,073,625 _RS14280 RS_RS14285: RS_RS14285 - MFS transporter, at 3,073,860 to 3,075,179 _RS14285 RS_RS14290: RS_RS14290 - long-chain-fatty-acid--CoA ligase, at 3,075,262 to 3,076,971 _RS14290 Position (kb) 3073 3074 3075 3076Strain fitness (log2 ratio) -1 0 1at 3073.047 kb on + strand, within RS_RS14280at 3073.047 kb on + strand, within RS_RS14280at 3073.047 kb on + strand, within RS_RS14280at 3073.048 kb on - strand, within RS_RS14280at 3073.081 kb on - strand, within RS_RS14280at 3073.173 kb on + strand, within RS_RS14280at 3073.174 kb on - strand, within RS_RS14280at 3073.335 kb on + strand, within RS_RS14280at 3073.339 kb on - strand, within RS_RS14280at 3073.419 kb on + strandat 3073.419 kb on + strand, within RS_RS14280at 3073.420 kb on - strand, within RS_RS14280at 3073.436 kb on + strand, within RS_RS14280at 3073.437 kb on - strand, within RS_RS14280at 3073.446 kb on + strand, within RS_RS14280at 3073.447 kb on - strand, within RS_RS14280at 3073.561 kb on - strandat 3073.975 kb on - strandat 3073.983 kb on + strandat 3074.217 kb on + strand, within RS_RS14285at 3074.508 kb on + strand, within RS_RS14285at 3074.509 kb on - strand, within RS_RS14285at 3074.509 kb on - strand, within RS_RS14285at 3074.650 kb on - strand, within RS_RS14285at 3074.661 kb on + strand, within RS_RS14285at 3074.661 kb on + strand, within RS_RS14285at 3074.661 kb on + strand, within RS_RS14285at 3074.661 kb on + strand, within RS_RS14285at 3074.662 kb on - strand, within RS_RS14285at 3074.663 kb on + strand, within RS_RS14285at 3074.664 kb on - strand, within RS_RS14285at 3074.688 kb on + strand, within RS_RS14285at 3074.688 kb on + strand, within RS_RS14285at 3074.991 kb on + strand, within RS_RS14285at 3075.013 kb on - strand, within RS_RS14285at 3075.051 kb on + strandat 3075.102 kb on + strandat 3075.102 kb on + strandat 3075.140 kb on - strandat 3075.368 kb on + strandat 3075.653 kb on + strand, within RS_RS14290at 3075.653 kb on + strand, within RS_RS14290at 3075.654 kb on - strand, within RS_RS14290at 3076.013 kb on + strand, within RS_RS14290at 3076.013 kb on + strand, within RS_RS14290at 3076.163 kb on + strand, within RS_RS14290at 3076.164 kb on - strand, within RS_RS14290

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,073,047 + RS_RS14280 0.51 +0.1
3,073,047 + RS_RS14280 0.51 +0.1
3,073,047 + RS_RS14280 0.51 -0.7
3,073,048 - RS_RS14280 0.51 -0.4
3,073,081 - RS_RS14280 0.53 -1.5
3,073,173 + RS_RS14280 0.61 -0.3
3,073,174 - RS_RS14280 0.61 -0.6
3,073,335 + RS_RS14280 0.75 +1.4
3,073,339 - RS_RS14280 0.75 -0.6
3,073,419 + +0.4
3,073,419 + RS_RS14280 0.82 -0.9
3,073,420 - RS_RS14280 0.82 -0.2
3,073,436 + RS_RS14280 0.84 +0.4
3,073,437 - RS_RS14280 0.84 +0.3
3,073,446 + RS_RS14280 0.85 -0.5
3,073,447 - RS_RS14280 0.85 -0.3
3,073,561 - +0.0
3,073,975 - -0.2
3,073,983 + -0.4
3,074,217 + RS_RS14285 0.27 +1.2
3,074,508 + RS_RS14285 0.49 +0.2
3,074,509 - RS_RS14285 0.49 -0.8
3,074,509 - RS_RS14285 0.49 +0.7
3,074,650 - RS_RS14285 0.60 -0.5
3,074,661 + RS_RS14285 0.61 -0.1
3,074,661 + RS_RS14285 0.61 -0.3
3,074,661 + RS_RS14285 0.61 +0.0
3,074,661 + RS_RS14285 0.61 +1.4
3,074,662 - RS_RS14285 0.61 +0.2
3,074,663 + RS_RS14285 0.61 -0.4
3,074,664 - RS_RS14285 0.61 +0.0
3,074,688 + RS_RS14285 0.63 +0.1
3,074,688 + RS_RS14285 0.63 -0.0
3,074,991 + RS_RS14285 0.86 -0.0
3,075,013 - RS_RS14285 0.87 -0.3
3,075,051 + +0.2
3,075,102 + -0.0
3,075,102 + -0.2
3,075,140 - -0.7
3,075,368 + +1.1
3,075,653 + RS_RS14290 0.23 -0.5
3,075,653 + RS_RS14290 0.23 +0.4
3,075,654 - RS_RS14290 0.23 +0.9
3,076,013 + RS_RS14290 0.44 +0.8
3,076,013 + RS_RS14290 0.44 -0.4
3,076,163 + RS_RS14290 0.53 +0.6
3,076,164 - RS_RS14290 0.53 +0.7

Or see this region's nucleotide sequence