Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS10775 and RS_RS10780 are separated by 17 nucleotides RS_RS10780 and RS_RS10785 are separated by 102 nucleotides RS_RS10785 and RS_RS10790 are separated by 302 nucleotides
RS_RS10775: RS_RS10775 - esterase, at 2,332,231 to 2,333,262
_RS10775
RS_RS10780: RS_RS10780 - hypothetical protein, at 2,333,280 to 2,333,585
_RS10780
RS_RS10785: RS_RS10785 - hypothetical protein, at 2,333,688 to 2,334,089
_RS10785
RS_RS10790: RS_RS10790 - transcription elongation factor GreB, at 2,334,392 to 2,334,955
_RS10790
Position (kb)
2333
2334 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2332.396 kb on + strand, within RS_RS10775 at 2332.396 kb on + strand, within RS_RS10775 at 2332.397 kb on - strand, within RS_RS10775 at 2332.397 kb on - strand, within RS_RS10775 at 2332.448 kb on - strand, within RS_RS10775 at 2332.536 kb on + strand, within RS_RS10775 at 2332.537 kb on - strand, within RS_RS10775 at 2332.630 kb on + strand, within RS_RS10775 at 2332.630 kb on + strand, within RS_RS10775 at 2332.631 kb on - strand, within RS_RS10775 at 2332.927 kb on + strand, within RS_RS10775 at 2332.928 kb on - strand, within RS_RS10775 at 2332.928 kb on - strand, within RS_RS10775 at 2333.534 kb on - strand, within RS_RS10780 at 2333.534 kb on - strand, within RS_RS10780 at 2333.534 kb on - strand, within RS_RS10780 at 2333.836 kb on + strand, within RS_RS10785 at 2333.884 kb on + strand, within RS_RS10785 at 2333.884 kb on + strand, within RS_RS10785 at 2333.885 kb on - strand, within RS_RS10785 at 2333.885 kb on - strand, within RS_RS10785 at 2333.885 kb on - strand, within RS_RS10785 at 2333.927 kb on - strand, within RS_RS10785 at 2333.956 kb on + strand, within RS_RS10785 at 2333.957 kb on - strand, within RS_RS10785 at 2334.075 kb on + strand at 2334.075 kb on + strand at 2334.075 kb on + strand at 2334.075 kb on + strand at 2334.075 kb on + strand at 2334.076 kb on - strand at 2334.076 kb on - strand at 2334.076 kb on - strand at 2334.076 kb on - strand at 2334.125 kb on + strand at 2334.159 kb on - strand at 2334.393 kb on + strand at 2334.393 kb on + strand at 2334.394 kb on - strand at 2334.394 kb on - strand at 2334.411 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,332,396 + RS_RS10775 0.16 +2.8 2,332,396 + RS_RS10775 0.16 +0.1 2,332,397 - RS_RS10775 0.16 +1.3 2,332,397 - RS_RS10775 0.16 +0.1 2,332,448 - RS_RS10775 0.21 -0.4 2,332,536 + RS_RS10775 0.30 +0.6 2,332,537 - RS_RS10775 0.30 +0.5 2,332,630 + RS_RS10775 0.39 -0.1 2,332,630 + RS_RS10775 0.39 -0.3 2,332,631 - RS_RS10775 0.39 -0.0 2,332,927 + RS_RS10775 0.67 +0.3 2,332,928 - RS_RS10775 0.68 +0.9 2,332,928 - RS_RS10775 0.68 +0.6 2,333,534 - RS_RS10780 0.83 -0.8 2,333,534 - RS_RS10780 0.83 +0.4 2,333,534 - RS_RS10780 0.83 +0.1 2,333,836 + RS_RS10785 0.37 +0.2 2,333,884 + RS_RS10785 0.49 +1.6 2,333,884 + RS_RS10785 0.49 +0.5 2,333,885 - RS_RS10785 0.49 -0.0 2,333,885 - RS_RS10785 0.49 +0.9 2,333,885 - RS_RS10785 0.49 +0.4 2,333,927 - RS_RS10785 0.59 -0.3 2,333,956 + RS_RS10785 0.67 -0.3 2,333,957 - RS_RS10785 0.67 -0.0 2,334,075 + +0.2 2,334,075 + +1.2 2,334,075 + -0.4 2,334,075 + -1.0 2,334,075 + +0.7 2,334,076 - -1.2 2,334,076 - -1.4 2,334,076 - +0.2 2,334,076 - -0.7 2,334,125 + -0.2 2,334,159 - -0.1 2,334,393 + +1.3 2,334,393 + +0.3 2,334,394 - +0.9 2,334,394 - +1.0 2,334,411 + -0.5
Or see this region's nucleotide sequence