Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS06230

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS06225 and RS_RS06230 are separated by 29 nucleotidesRS_RS06230 and RS_RS06235 are separated by 69 nucleotides RS_RS06225: RS_RS06225 - sugar ABC transporter ATP-binding protein, at 1,316,871 to 1,318,400 _RS06225 RS_RS06230: RS_RS06230 - ABC transporter permease, at 1,318,430 to 1,319,455 _RS06230 RS_RS06235: RS_RS06235 - sugar phosphate isomerase/epimerase, at 1,319,525 to 1,320,436 _RS06235 Position (kb) 1318 1319 1320Strain fitness (log2 ratio) -1 0 1at 1317.997 kb on - strand, within RS_RS06225at 1317.997 kb on - strand, within RS_RS06225at 1319.049 kb on - strand, within RS_RS06230at 1319.072 kb on + strand, within RS_RS06230at 1319.072 kb on + strand, within RS_RS06230at 1319.072 kb on + strand, within RS_RS06230at 1319.073 kb on - strand, within RS_RS06230at 1319.231 kb on + strand, within RS_RS06230at 1319.232 kb on - strand, within RS_RS06230at 1319.464 kb on + strandat 1319.528 kb on + strandat 1319.546 kb on + strandat 1319.546 kb on + strandat 1319.546 kb on + strandat 1319.547 kb on - strandat 1319.741 kb on + strand, within RS_RS06235at 1319.741 kb on + strand, within RS_RS06235at 1319.742 kb on - strand, within RS_RS06235at 1319.742 kb on - strand, within RS_RS06235at 1320.078 kb on - strand, within RS_RS06235at 1320.098 kb on + strand, within RS_RS06235at 1320.203 kb on + strand, within RS_RS06235at 1320.240 kb on + strand, within RS_RS06235at 1320.402 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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1,317,997 - RS_RS06225 0.74 -0.1
1,317,997 - RS_RS06225 0.74 -0.5
1,319,049 - RS_RS06230 0.60 +0.2
1,319,072 + RS_RS06230 0.63 +1.1
1,319,072 + RS_RS06230 0.63 -0.4
1,319,072 + RS_RS06230 0.63 +1.4
1,319,073 - RS_RS06230 0.63 +0.1
1,319,231 + RS_RS06230 0.78 +0.6
1,319,232 - RS_RS06230 0.78 +0.5
1,319,464 + -1.3
1,319,528 + -0.0
1,319,546 + -1.3
1,319,546 + -0.5
1,319,546 + -1.3
1,319,547 - +0.1
1,319,741 + RS_RS06235 0.24 -0.3
1,319,741 + RS_RS06235 0.24 -0.7
1,319,742 - RS_RS06235 0.24 -0.4
1,319,742 - RS_RS06235 0.24 -0.9
1,320,078 - RS_RS06235 0.61 -1.2
1,320,098 + RS_RS06235 0.63 +0.1
1,320,203 + RS_RS06235 0.74 +0.5
1,320,240 + RS_RS06235 0.78 -0.5
1,320,402 - -0.3

Or see this region's nucleotide sequence