Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS03545 and RS_RS03550 are separated by 58 nucleotides RS_RS03550 and RS_RS03555 are separated by 42 nucleotides RS_RS03555 and RS_RS25345 are separated by 149 nucleotides
RS_RS03545: RS_RS03545 - carbon-nitrogen hydrolase family protein, at 751,671 to 752,420
_RS03545
RS_RS03550: RS_RS03550 - DUF4337 domain-containing protein, at 752,479 to 753,051
_RS03550
RS_RS03555: RS_RS03555 - hypothetical protein, at 753,094 to 753,690
_RS03555
RS_RS25345: RS_RS25345 - hypothetical protein, at 753,840 to 754,448
_RS25345
Position (kb) 752
753
754 Strain fitness (log2 ratio) -1
0
1 at 751.898 kb on - strand, within RS_RS03545 at 751.930 kb on + strand, within RS_RS03545 at 751.930 kb on + strand, within RS_RS03545 at 751.930 kb on + strand, within RS_RS03545 at 752.093 kb on - strand, within RS_RS03545 at 752.450 kb on + strand at 752.450 kb on + strand at 752.730 kb on - strand, within RS_RS03550 at 752.822 kb on + strand, within RS_RS03550 at 752.823 kb on - strand, within RS_RS03550 at 752.825 kb on + strand, within RS_RS03550 at 752.826 kb on - strand, within RS_RS03550 at 753.095 kb on + strand at 753.095 kb on + strand at 753.096 kb on - strand at 753.096 kb on - strand at 753.096 kb on - strand at 753.261 kb on - strand, within RS_RS03555 at 753.282 kb on - strand, within RS_RS03555 at 753.344 kb on + strand, within RS_RS03555 at 753.345 kb on - strand, within RS_RS03555 at 753.348 kb on - strand, within RS_RS03555 at 753.515 kb on + strand, within RS_RS03555 at 753.515 kb on + strand, within RS_RS03555 at 753.515 kb on + strand, within RS_RS03555 at 753.515 kb on + strand, within RS_RS03555 at 753.515 kb on + strand, within RS_RS03555 at 753.516 kb on - strand, within RS_RS03555 at 753.516 kb on - strand, within RS_RS03555 at 753.745 kb on + strand at 753.745 kb on + strand at 753.745 kb on + strand at 753.745 kb on + strand at 753.746 kb on - strand at 753.746 kb on - strand at 753.813 kb on + strand at 753.814 kb on - strand at 753.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 751,898 - RS_RS03545 0.30 +0.6 751,930 + RS_RS03545 0.35 -0.1 751,930 + RS_RS03545 0.35 -0.3 751,930 + RS_RS03545 0.35 -0.5 752,093 - RS_RS03545 0.56 -0.8 752,450 + -0.6 752,450 + -0.1 752,730 - RS_RS03550 0.44 +0.6 752,822 + RS_RS03550 0.60 -0.2 752,823 - RS_RS03550 0.60 -0.7 752,825 + RS_RS03550 0.60 -0.1 752,826 - RS_RS03550 0.61 +0.7 753,095 + +0.7 753,095 + +1.3 753,096 - -0.9 753,096 - -0.2 753,096 - +0.0 753,261 - RS_RS03555 0.28 +0.4 753,282 - RS_RS03555 0.31 -0.4 753,344 + RS_RS03555 0.42 +0.0 753,345 - RS_RS03555 0.42 -0.4 753,348 - RS_RS03555 0.43 -0.1 753,515 + RS_RS03555 0.71 -0.6 753,515 + RS_RS03555 0.71 -0.3 753,515 + RS_RS03555 0.71 +0.2 753,515 + RS_RS03555 0.71 -0.2 753,515 + RS_RS03555 0.71 +0.8 753,516 - RS_RS03555 0.71 -0.5 753,516 - RS_RS03555 0.71 -0.3 753,745 + +0.0 753,745 + -0.2 753,745 + +0.2 753,745 + +1.3 753,746 - -0.2 753,746 - -0.2 753,813 + +0.1 753,814 - +0.0 753,868 + +0.0
Or see this region's nucleotide sequence