Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS23840 and RS_RS23845 overlap by 4 nucleotides RS_RS23845 and RS_RS23850 are separated by 206 nucleotides
RS_RS23840: RS_RS23840 - flagellar biosynthesis protein FlhF, at 1,752,159 to 1,754,006
_RS23840
RS_RS23845: RS_RS23845 - flagellar biosynthesis protein FlhA, at 1,754,003 to 1,756,093
_RS23845
RS_RS23850: RS_RS23850 - flagellar type III secretion system protein FlhB, at 1,756,300 to 1,757,463
_RS23850
Position (kb) 1754
1755
1756
1757 Strain fitness (log2 ratio) -1
0
1
2
3 at 1753.210 kb on + strand, within RS_RS23840 at 1754.035 kb on + strand at 1754.169 kb on + strand at 1754.593 kb on + strand, within RS_RS23845 at 1754.593 kb on + strand, within RS_RS23845 at 1754.593 kb on + strand, within RS_RS23845 at 1754.594 kb on - strand, within RS_RS23845 at 1754.594 kb on - strand, within RS_RS23845 at 1754.594 kb on - strand, within RS_RS23845 at 1754.602 kb on - strand, within RS_RS23845 at 1754.655 kb on + strand, within RS_RS23845 at 1754.656 kb on - strand, within RS_RS23845 at 1754.760 kb on + strand, within RS_RS23845 at 1754.885 kb on - strand, within RS_RS23845 at 1754.885 kb on - strand, within RS_RS23845 at 1755.135 kb on + strand, within RS_RS23845 at 1755.376 kb on - strand, within RS_RS23845 at 1755.376 kb on - strand, within RS_RS23845 at 1755.732 kb on + strand, within RS_RS23845 at 1755.732 kb on + strand, within RS_RS23845 at 1755.732 kb on + strand, within RS_RS23845 at 1755.733 kb on - strand, within RS_RS23845 at 1755.733 kb on - strand, within RS_RS23845 at 1755.733 kb on - strand, within RS_RS23845 at 1756.231 kb on + strand at 1756.233 kb on + strand at 1756.233 kb on + strand at 1756.233 kb on + strand at 1756.234 kb on - strand at 1756.234 kb on - strand at 1756.234 kb on - strand at 1756.234 kb on - strand at 1756.425 kb on - strand, within RS_RS23850 at 1756.430 kb on + strand, within RS_RS23850 at 1756.430 kb on + strand, within RS_RS23850 at 1756.431 kb on - strand, within RS_RS23850 at 1756.457 kb on + strand, within RS_RS23850 at 1756.457 kb on + strand, within RS_RS23850 at 1756.631 kb on + strand, within RS_RS23850 at 1756.649 kb on + strand, within RS_RS23850 at 1756.649 kb on + strand, within RS_RS23850 at 1756.650 kb on - strand, within RS_RS23850 at 1756.821 kb on - strand, within RS_RS23850 at 1756.821 kb on - strand, within RS_RS23850 at 1756.821 kb on - strand, within RS_RS23850
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,753,210 + RS_RS23840 0.57 -0.7 1,754,035 + -0.3 1,754,169 + -0.1 1,754,593 + RS_RS23845 0.28 +0.3 1,754,593 + RS_RS23845 0.28 +0.6 1,754,593 + RS_RS23845 0.28 +0.4 1,754,594 - RS_RS23845 0.28 +2.8 1,754,594 - RS_RS23845 0.28 +0.1 1,754,594 - RS_RS23845 0.28 -0.3 1,754,602 - RS_RS23845 0.29 -0.3 1,754,655 + RS_RS23845 0.31 -0.1 1,754,656 - RS_RS23845 0.31 -0.4 1,754,760 + RS_RS23845 0.36 -0.2 1,754,885 - RS_RS23845 0.42 +0.2 1,754,885 - RS_RS23845 0.42 +0.1 1,755,135 + RS_RS23845 0.54 +0.6 1,755,376 - RS_RS23845 0.66 -0.1 1,755,376 - RS_RS23845 0.66 -0.4 1,755,732 + RS_RS23845 0.83 -1.6 1,755,732 + RS_RS23845 0.83 +0.1 1,755,732 + RS_RS23845 0.83 +0.6 1,755,733 - RS_RS23845 0.83 +0.0 1,755,733 - RS_RS23845 0.83 -0.3 1,755,733 - RS_RS23845 0.83 -0.3 1,756,231 + +0.0 1,756,233 + -0.9 1,756,233 + +0.3 1,756,233 + -0.4 1,756,234 - +0.6 1,756,234 - -0.2 1,756,234 - -0.1 1,756,234 - +0.4 1,756,425 - RS_RS23850 0.11 -0.8 1,756,430 + RS_RS23850 0.11 -0.4 1,756,430 + RS_RS23850 0.11 +0.0 1,756,431 - RS_RS23850 0.11 +0.3 1,756,457 + RS_RS23850 0.13 -0.3 1,756,457 + RS_RS23850 0.13 -0.1 1,756,631 + RS_RS23850 0.28 +0.1 1,756,649 + RS_RS23850 0.30 -0.4 1,756,649 + RS_RS23850 0.30 +0.8 1,756,650 - RS_RS23850 0.30 -0.3 1,756,821 - RS_RS23850 0.45 +0.4 1,756,821 - RS_RS23850 0.45 -0.1 1,756,821 - RS_RS23850 0.45 +0.5
Or see this region's nucleotide sequence