Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS18780

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS18775 and RS_RS18780 are separated by 517 nucleotidesRS_RS18780 and RS_RS18785 are separated by 115 nucleotidesRS_RS18785 and RS_RS18790 are separated by 102 nucleotides RS_RS18775: RS_RS18775 - DNA-binding response regulator, at 444,706 to 445,386 _RS18775 RS_RS18780: RS_RS18780 - flagellar protein FlgN, at 445,904 to 446,365 _RS18780 RS_RS18785: RS_RS18785 - flagellar biosynthesis anti-sigma factor FlgM, at 446,481 to 446,801 _RS18785 RS_RS18790: RS_RS18790 - flagella basal body P-ring formation protein FlgA, at 446,904 to 447,791 _RS18790 Position (kb) 445 446 447Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 444.944 kb on + strand, within RS_RS18775at 445.011 kb on + strand, within RS_RS18775at 445.028 kb on - strand, within RS_RS18775at 445.042 kb on + strand, within RS_RS18775at 445.042 kb on + strand, within RS_RS18775at 445.046 kb on + strand, within RS_RS18775at 445.046 kb on + strand, within RS_RS18775at 445.046 kb on + strand, within RS_RS18775at 445.047 kb on - strand, within RS_RS18775at 445.047 kb on - strand, within RS_RS18775at 445.047 kb on - strand, within RS_RS18775at 445.064 kb on - strand, within RS_RS18775at 445.064 kb on - strand, within RS_RS18775at 445.066 kb on + strand, within RS_RS18775at 445.067 kb on - strand, within RS_RS18775at 445.100 kb on + strand, within RS_RS18775at 445.100 kb on + strand, within RS_RS18775at 445.100 kb on + strand, within RS_RS18775at 445.115 kb on + strand, within RS_RS18775at 445.115 kb on + strand, within RS_RS18775at 445.116 kb on - strand, within RS_RS18775at 445.125 kb on + strand, within RS_RS18775at 445.185 kb on + strand, within RS_RS18775at 445.185 kb on + strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.186 kb on - strand, within RS_RS18775at 445.230 kb on + strand, within RS_RS18775at 445.440 kb on + strandat 445.440 kb on + strandat 445.440 kb on + strandat 445.440 kb on + strandat 445.440 kb on + strandat 445.441 kb on - strandat 445.441 kb on - strandat 445.479 kb on + strandat 445.480 kb on - strandat 445.480 kb on - strandat 445.545 kb on - strandat 445.723 kb on + strandat 445.766 kb on + strandat 445.770 kb on + strandat 445.771 kb on - strandat 445.906 kb on - strandat 445.962 kb on + strand, within RS_RS18780at 445.962 kb on + strand, within RS_RS18780at 445.962 kb on + strand, within RS_RS18780at 445.963 kb on - strand, within RS_RS18780at 446.133 kb on + strand, within RS_RS18780at 446.134 kb on - strand, within RS_RS18780at 446.352 kb on - strandat 446.445 kb on + strandat 446.445 kb on + strandat 446.445 kb on + strandat 446.446 kb on - strandat 446.446 kb on - strandat 446.446 kb on - strandat 446.446 kb on - strandat 446.829 kb on + strandat 446.829 kb on + strandat 446.829 kb on + strandat 446.829 kb on + strandat 446.829 kb on + strandat 446.830 kb on - strandat 446.830 kb on - strandat 446.830 kb on - strandat 446.830 kb on - strandat 446.830 kb on - strandat 446.830 kb on - strandat 446.863 kb on + strandat 446.864 kb on - strandat 446.896 kb on + strandat 446.896 kb on + strandat 446.898 kb on + strandat 447.271 kb on + strand, within RS_RS18790at 447.271 kb on + strand, within RS_RS18790at 447.271 kb on + strand, within RS_RS18790at 447.272 kb on - strand, within RS_RS18790at 447.272 kb on - strand, within RS_RS18790at 447.274 kb on + strand, within RS_RS18790at 447.274 kb on + strand, within RS_RS18790at 447.274 kb on + strand, within RS_RS18790at 447.274 kb on + strand, within RS_RS18790at 447.274 kb on + strand, within RS_RS18790at 447.275 kb on - strand, within RS_RS18790at 447.275 kb on - strand, within RS_RS18790at 447.275 kb on - strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.351 kb on + strand, within RS_RS18790at 447.352 kb on - strand, within RS_RS18790at 447.352 kb on - strand, within RS_RS18790at 447.352 kb on - strand, within RS_RS18790at 447.352 kb on - strand, within RS_RS18790at 447.352 kb on - strand, within RS_RS18790

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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444,944 + RS_RS18775 0.35 +0.8
445,011 + RS_RS18775 0.45 +0.5
445,028 - RS_RS18775 0.47 +0.8
445,042 + RS_RS18775 0.49 +0.7
445,042 + RS_RS18775 0.49 +0.2
445,046 + RS_RS18775 0.50 +0.6
445,046 + RS_RS18775 0.50 -0.1
445,046 + RS_RS18775 0.50 +0.1
445,047 - RS_RS18775 0.50 +0.9
445,047 - RS_RS18775 0.50 +1.5
445,047 - RS_RS18775 0.50 +0.8
445,064 - RS_RS18775 0.53 +1.0
445,064 - RS_RS18775 0.53 +0.8
445,066 + RS_RS18775 0.53 +0.3
445,067 - RS_RS18775 0.53 +0.7
445,100 + RS_RS18775 0.58 +0.6
445,100 + RS_RS18775 0.58 +0.6
445,100 + RS_RS18775 0.58 +0.8
445,115 + RS_RS18775 0.60 +0.7
445,115 + RS_RS18775 0.60 +0.7
445,116 - RS_RS18775 0.60 +1.1
445,125 + RS_RS18775 0.62 +1.0
445,185 + RS_RS18775 0.70 +0.9
445,185 + RS_RS18775 0.70 +0.5
445,186 - RS_RS18775 0.70 +0.6
445,186 - RS_RS18775 0.70 +0.8
445,186 - RS_RS18775 0.70 +1.3
445,186 - RS_RS18775 0.70 +0.6
445,186 - RS_RS18775 0.70 +0.3
445,186 - RS_RS18775 0.70 +1.1
445,230 + RS_RS18775 0.77 -1.8
445,440 + +0.4
445,440 + +0.2
445,440 + +0.5
445,440 + +0.7
445,440 + +1.2
445,441 - -0.7
445,441 - +1.6
445,479 + +0.1
445,480 - -0.1
445,480 - -1.0
445,545 - -0.9
445,723 + +0.5
445,766 + +0.3
445,770 + +0.4
445,771 - -1.3
445,906 - +4.6
445,962 + RS_RS18780 0.13 +0.4
445,962 + RS_RS18780 0.13 +0.1
445,962 + RS_RS18780 0.13 +0.7
445,963 - RS_RS18780 0.13 +0.4
446,133 + RS_RS18780 0.50 +0.2
446,134 - RS_RS18780 0.50 +0.2
446,352 - -0.1
446,445 + +1.9
446,445 + -0.1
446,445 + -0.2
446,446 - -0.0
446,446 - +0.4
446,446 - -0.8
446,446 - +0.2
446,829 + -0.3
446,829 + +0.7
446,829 + +0.7
446,829 + +0.5
446,829 + -0.1
446,830 - +1.3
446,830 - +0.7
446,830 - +0.0
446,830 - +0.7
446,830 - +0.1
446,830 - +0.2
446,863 + +0.2
446,864 - +0.4
446,896 + +0.6
446,896 + +0.0
446,898 + -0.2
447,271 + RS_RS18790 0.41 -0.3
447,271 + RS_RS18790 0.41 -0.3
447,271 + RS_RS18790 0.41 +0.4
447,272 - RS_RS18790 0.41 +0.0
447,272 - RS_RS18790 0.41 +0.4
447,274 + RS_RS18790 0.42 -0.2
447,274 + RS_RS18790 0.42 +0.5
447,274 + RS_RS18790 0.42 +0.0
447,274 + RS_RS18790 0.42 -0.7
447,274 + RS_RS18790 0.42 +0.6
447,275 - RS_RS18790 0.42 -0.7
447,275 - RS_RS18790 0.42 +0.3
447,275 - RS_RS18790 0.42 -0.1
447,351 + RS_RS18790 0.50 -0.8
447,351 + RS_RS18790 0.50 -0.4
447,351 + RS_RS18790 0.50 +0.2
447,351 + RS_RS18790 0.50 -0.1
447,351 + RS_RS18790 0.50 -0.2
447,351 + RS_RS18790 0.50 +0.0
447,351 + RS_RS18790 0.50 +1.6
447,352 - RS_RS18790 0.50 -0.0
447,352 - RS_RS18790 0.50 +0.3
447,352 - RS_RS18790 0.50 +0.3
447,352 - RS_RS18790 0.50 -0.1
447,352 - RS_RS18790 0.50 +0.0

Or see this region's nucleotide sequence