Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17730 and RS_RS17735 are separated by 87 nucleotides RS_RS17735 and RS_RS17740 are separated by 51 nucleotides
RS_RS17730: RS_RS17730 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 136,989 to 139,586
_RS17730
RS_RS17735: RS_RS17735 - 2-methylcitrate synthase, at 139,674 to 140,837
_RS17735
RS_RS17740: RS_RS17740 - methylisocitrate lyase, at 140,889 to 141,785
_RS17740
Position (kb) 139
140
141 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 138.694 kb on + strand, within RS_RS17730 at 139.480 kb on + strand at 139.496 kb on - strand at 139.496 kb on - strand at 139.541 kb on - strand at 139.541 kb on - strand at 139.541 kb on - strand at 139.574 kb on - strand at 139.720 kb on + strand at 139.720 kb on + strand at 139.847 kb on - strand, within RS_RS17735 at 139.847 kb on - strand, within RS_RS17735 at 139.847 kb on - strand, within RS_RS17735 at 140.011 kb on + strand, within RS_RS17735 at 140.012 kb on - strand, within RS_RS17735 at 140.012 kb on - strand, within RS_RS17735 at 140.182 kb on + strand, within RS_RS17735 at 140.182 kb on + strand, within RS_RS17735 at 140.182 kb on + strand, within RS_RS17735 at 140.182 kb on + strand, within RS_RS17735 at 140.183 kb on - strand, within RS_RS17735 at 140.251 kb on + strand, within RS_RS17735 at 140.383 kb on + strand, within RS_RS17735 at 140.383 kb on + strand, within RS_RS17735 at 140.383 kb on + strand, within RS_RS17735 at 140.389 kb on + strand, within RS_RS17735 at 140.389 kb on + strand, within RS_RS17735 at 140.390 kb on - strand, within RS_RS17735 at 140.395 kb on + strand, within RS_RS17735 at 140.396 kb on - strand, within RS_RS17735 at 140.396 kb on - strand, within RS_RS17735 at 140.605 kb on + strand, within RS_RS17735 at 140.605 kb on + strand, within RS_RS17735 at 140.605 kb on + strand, within RS_RS17735 at 140.605 kb on + strand, within RS_RS17735 at 140.605 kb on + strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.606 kb on - strand, within RS_RS17735 at 140.653 kb on + strand, within RS_RS17735 at 140.891 kb on - strand at 140.891 kb on - strand at 140.947 kb on + strand at 140.947 kb on + strand at 140.948 kb on - strand at 140.954 kb on - strand at 141.022 kb on + strand, within RS_RS17740 at 141.055 kb on + strand, within RS_RS17740 at 141.244 kb on + strand, within RS_RS17740 at 141.244 kb on + strand, within RS_RS17740 at 141.244 kb on + strand, within RS_RS17740 at 141.643 kb on + strand, within RS_RS17740 at 141.644 kb on - strand, within RS_RS17740 at 141.644 kb on - strand, within RS_RS17740 at 141.644 kb on - strand, within RS_RS17740 at 141.644 kb on - strand, within RS_RS17740 at 141.685 kb on + strand, within RS_RS17740
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 138,694 + RS_RS17730 0.66 -0.5 139,480 + -0.6 139,496 - -0.7 139,496 - -1.1 139,541 - -3.1 139,541 - -1.0 139,541 - -0.2 139,574 - -0.5 139,720 + +0.6 139,720 + +0.6 139,847 - RS_RS17735 0.15 -0.7 139,847 - RS_RS17735 0.15 +1.3 139,847 - RS_RS17735 0.15 -0.5 140,011 + RS_RS17735 0.29 +0.6 140,012 - RS_RS17735 0.29 -1.4 140,012 - RS_RS17735 0.29 -0.6 140,182 + RS_RS17735 0.44 +0.4 140,182 + RS_RS17735 0.44 +0.6 140,182 + RS_RS17735 0.44 +0.8 140,182 + RS_RS17735 0.44 +0.7 140,183 - RS_RS17735 0.44 -0.4 140,251 + RS_RS17735 0.50 -0.5 140,383 + RS_RS17735 0.61 +1.8 140,383 + RS_RS17735 0.61 +0.3 140,383 + RS_RS17735 0.61 +1.1 140,389 + RS_RS17735 0.61 +0.6 140,389 + RS_RS17735 0.61 +1.6 140,390 - RS_RS17735 0.62 -0.2 140,395 + RS_RS17735 0.62 +0.5 140,396 - RS_RS17735 0.62 -2.1 140,396 - RS_RS17735 0.62 -0.9 140,605 + RS_RS17735 0.80 +0.8 140,605 + RS_RS17735 0.80 +0.6 140,605 + RS_RS17735 0.80 +1.0 140,605 + RS_RS17735 0.80 +1.1 140,605 + RS_RS17735 0.80 +0.8 140,606 - RS_RS17735 0.80 -0.8 140,606 - RS_RS17735 0.80 +0.1 140,606 - RS_RS17735 0.80 +0.5 140,606 - RS_RS17735 0.80 -0.0 140,606 - RS_RS17735 0.80 -0.4 140,606 - RS_RS17735 0.80 +1.2 140,653 + RS_RS17735 0.84 +0.8 140,891 - +0.0 140,891 - +1.3 140,947 + +0.6 140,947 + +1.0 140,948 - +0.2 140,954 - +0.6 141,022 + RS_RS17740 0.15 +0.1 141,055 + RS_RS17740 0.19 +1.0 141,244 + RS_RS17740 0.40 +1.0 141,244 + RS_RS17740 0.40 +0.6 141,244 + RS_RS17740 0.40 +0.8 141,643 + RS_RS17740 0.84 +1.3 141,644 - RS_RS17740 0.84 +0.1 141,644 - RS_RS17740 0.84 +0.0 141,644 - RS_RS17740 0.84 -0.5 141,644 - RS_RS17740 0.84 -0.2 141,685 + RS_RS17740 0.89 +1.1
Or see this region's nucleotide sequence