Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17320 and RS_RS17325 are separated by 415 nucleotides RS_RS17325 and RS_RS17330 are separated by 469 nucleotides
RS_RS17320: RS_RS17320 - divalent metal cation transporter, at 29,714 to 31,357
_RS17320
RS_RS17325: RS_RS17325 - GALA protein, at 31,773 to 33,329
_RS17325
RS_RS17330: RS_RS17330 - DNA-binding protein, at 33,799 to 34,086
_RS17330
Position (kb) 31
32
33
34 Strain fitness (log2 ratio) -2
-1
0
1
2 at 30.809 kb on + strand, within RS_RS17320 at 30.809 kb on + strand, within RS_RS17320 at 30.810 kb on - strand, within RS_RS17320 at 31.474 kb on + strand at 31.475 kb on - strand at 31.475 kb on - strand at 31.475 kb on - strand at 31.947 kb on - strand, within RS_RS17325 at 32.034 kb on + strand, within RS_RS17325 at 32.133 kb on - strand, within RS_RS17325 at 32.133 kb on - strand, within RS_RS17325 at 32.301 kb on + strand, within RS_RS17325 at 32.301 kb on + strand, within RS_RS17325 at 32.301 kb on + strand, within RS_RS17325 at 32.302 kb on - strand, within RS_RS17325 at 32.775 kb on + strand, within RS_RS17325 at 32.775 kb on + strand, within RS_RS17325 at 32.964 kb on + strand, within RS_RS17325 at 32.964 kb on + strand, within RS_RS17325 at 32.965 kb on - strand, within RS_RS17325 at 32.965 kb on - strand, within RS_RS17325 at 32.965 kb on - strand, within RS_RS17325 at 33.062 kb on - strand, within RS_RS17325 at 33.062 kb on - strand, within RS_RS17325 at 33.062 kb on - strand, within RS_RS17325 at 33.890 kb on + strand, within RS_RS17330 at 33.890 kb on + strand, within RS_RS17330 at 33.969 kb on - strand, within RS_RS17330 at 34.076 kb on + strand at 34.076 kb on + strand at 34.076 kb on + strand at 34.077 kb on - strand at 34.121 kb on - strand at 34.199 kb on - strand at 34.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 30,809 + RS_RS17320 0.67 +0.0 30,809 + RS_RS17320 0.67 +0.2 30,810 - RS_RS17320 0.67 +1.9 31,474 + +0.3 31,475 - -1.7 31,475 - +1.0 31,475 - -0.2 31,947 - RS_RS17325 0.11 +2.0 32,034 + RS_RS17325 0.17 +0.2 32,133 - RS_RS17325 0.23 -0.4 32,133 - RS_RS17325 0.23 -1.0 32,301 + RS_RS17325 0.34 -1.2 32,301 + RS_RS17325 0.34 +0.8 32,301 + RS_RS17325 0.34 -0.6 32,302 - RS_RS17325 0.34 -0.8 32,775 + RS_RS17325 0.64 +0.5 32,775 + RS_RS17325 0.64 +0.2 32,964 + RS_RS17325 0.76 +0.6 32,964 + RS_RS17325 0.76 +0.4 32,965 - RS_RS17325 0.77 -1.2 32,965 - RS_RS17325 0.77 -0.7 32,965 - RS_RS17325 0.77 +0.0 33,062 - RS_RS17325 0.83 -1.0 33,062 - RS_RS17325 0.83 -0.4 33,062 - RS_RS17325 0.83 +0.4 33,890 + RS_RS17330 0.32 -1.0 33,890 + RS_RS17330 0.32 -1.8 33,969 - RS_RS17330 0.59 +1.6 34,076 + -1.9 34,076 + -0.2 34,076 + +1.6 34,077 - +1.6 34,121 - +0.3 34,199 - -0.4 34,234 - +0.4
Or see this region's nucleotide sequence