Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17260 and RS_RS17265 are separated by 96 nucleotides RS_RS17265 and RS_RS17270 are separated by 10 nucleotides RS_RS17270 and RS_RS17275 are separated by 7 nucleotides RS_RS17275 and RS_RS17280 overlap by 4 nucleotides
RS_RS17260: RS_RS17260 - amino acid ABC transporter substrate-binding protein, at 17,329 to 18,489
_RS17260
RS_RS17265: RS_RS17265 - branched-chain amino acid ABC transporter permease, at 18,586 to 19,467
_RS17265
RS_RS17270: RS_RS17270 - branched-chain amino acid ABC transporter permease, at 19,478 to 20,377
_RS17270
RS_RS17275: RS_RS17275 - ABC transporter ATP-binding protein, at 20,385 to 21,182
_RS17275
RS_RS17280: RS_RS17280 - ABC transporter ATP-binding protein, at 21,179 to 21,904
_RS17280
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1 at 18.487 kb on + strand at 18.487 kb on + strand at 18.524 kb on + strand at 18.524 kb on + strand at 18.524 kb on + strand at 18.524 kb on + strand at 18.525 kb on - strand at 18.525 kb on - strand at 18.631 kb on + strand at 18.631 kb on + strand at 18.631 kb on + strand at 18.632 kb on - strand at 18.632 kb on - strand at 19.217 kb on - strand, within RS_RS17265 at 19.360 kb on + strand, within RS_RS17265 at 19.508 kb on + strand at 19.508 kb on + strand at 19.562 kb on + strand at 19.563 kb on - strand at 19.625 kb on + strand, within RS_RS17270 at 19.626 kb on - strand, within RS_RS17270 at 19.626 kb on - strand, within RS_RS17270 at 19.752 kb on - strand, within RS_RS17270 at 19.752 kb on - strand, within RS_RS17270 at 19.804 kb on + strand, within RS_RS17270 at 19.805 kb on - strand, within RS_RS17270 at 19.805 kb on - strand, within RS_RS17270 at 20.363 kb on - strand at 20.363 kb on - strand at 20.839 kb on - strand, within RS_RS17275 at 20.839 kb on - strand, within RS_RS17275 at 20.982 kb on + strand, within RS_RS17275 at 20.982 kb on + strand, within RS_RS17275 at 21.120 kb on + strand at 21.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 18,487 + +1.6 18,487 + -0.4 18,524 + -0.1 18,524 + -1.0 18,524 + -0.1 18,524 + -0.2 18,525 - +0.0 18,525 - -0.4 18,631 + -0.1 18,631 + -0.1 18,631 + +0.0 18,632 - -0.0 18,632 - -0.4 19,217 - RS_RS17265 0.72 -0.1 19,360 + RS_RS17265 0.88 +0.6 19,508 + +0.0 19,508 + -0.2 19,562 + -0.6 19,563 - -1.1 19,625 + RS_RS17270 0.16 -0.0 19,626 - RS_RS17270 0.16 +0.6 19,626 - RS_RS17270 0.16 -0.5 19,752 - RS_RS17270 0.30 -0.0 19,752 - RS_RS17270 0.30 +1.6 19,804 + RS_RS17270 0.36 -0.2 19,805 - RS_RS17270 0.36 -0.3 19,805 - RS_RS17270 0.36 -0.7 20,363 - -0.3 20,363 - -0.3 20,839 - RS_RS17275 0.57 +0.2 20,839 - RS_RS17275 0.57 -0.5 20,982 + RS_RS17275 0.75 -0.6 20,982 + RS_RS17275 0.75 -1.2 21,120 + -0.3 21,219 - -0.3
Or see this region's nucleotide sequence