Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17245 and RS_RS17250 are separated by 170 nucleotides RS_RS17250 and RS_RS17255 are separated by 94 nucleotides RS_RS17255 and RS_RS17260 are separated by 481 nucleotides
RS_RS17245: RS_RS17245 - YhfC family intramembrane metalloprotease, at 12,657 to 13,454
_RS17245
RS_RS17250: RS_RS17250 - hypothetical protein, at 13,625 to 14,056
_RS17250
RS_RS17255: RS_RS17255 - PAS domain S-box protein, at 14,151 to 16,847
_RS17255
RS_RS17260: RS_RS17260 - amino acid ABC transporter substrate-binding protein, at 17,329 to 18,489
_RS17260
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -1
0
1 at 13.210 kb on + strand, within RS_RS17245 at 13.211 kb on - strand, within RS_RS17245 at 13.211 kb on - strand, within RS_RS17245 at 13.273 kb on + strand, within RS_RS17245 at 13.273 kb on + strand, within RS_RS17245 at 13.486 kb on - strand at 13.570 kb on + strand at 13.571 kb on - strand at 13.575 kb on + strand at 13.575 kb on + strand at 13.575 kb on + strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.576 kb on - strand at 13.577 kb on + strand at 13.578 kb on - strand at 13.730 kb on + strand, within RS_RS17250 at 13.730 kb on + strand, within RS_RS17250 at 13.731 kb on - strand, within RS_RS17250 at 13.931 kb on + strand, within RS_RS17250 at 13.931 kb on + strand, within RS_RS17250 at 13.931 kb on + strand, within RS_RS17250 at 13.932 kb on - strand, within RS_RS17250 at 13.932 kb on - strand, within RS_RS17250 at 13.932 kb on - strand, within RS_RS17250 at 13.932 kb on - strand, within RS_RS17250 at 14.036 kb on + strand at 14.036 kb on + strand at 14.036 kb on + strand at 14.037 kb on - strand at 14.334 kb on + strand at 14.334 kb on + strand at 14.334 kb on + strand at 14.334 kb on + strand at 14.334 kb on + strand at 14.334 kb on + strand at 14.335 kb on - strand at 14.335 kb on - strand at 14.335 kb on - strand at 14.496 kb on + strand, within RS_RS17255 at 14.682 kb on + strand, within RS_RS17255 at 14.683 kb on - strand, within RS_RS17255 at 14.749 kb on - strand, within RS_RS17255 at 14.749 kb on - strand, within RS_RS17255 at 14.749 kb on - strand, within RS_RS17255 at 15.285 kb on + strand, within RS_RS17255 at 15.285 kb on + strand, within RS_RS17255 at 15.286 kb on - strand, within RS_RS17255 at 15.648 kb on + strand, within RS_RS17255 at 15.648 kb on + strand, within RS_RS17255 at 15.648 kb on + strand, within RS_RS17255 at 15.648 kb on + strand, within RS_RS17255 at 15.649 kb on - strand, within RS_RS17255 at 15.649 kb on - strand, within RS_RS17255 at 15.649 kb on - strand, within RS_RS17255 at 15.650 kb on + strand, within RS_RS17255 at 15.650 kb on + strand, within RS_RS17255 at 15.651 kb on - strand, within RS_RS17255 at 16.119 kb on + strand, within RS_RS17255 at 16.470 kb on - strand, within RS_RS17255 at 16.770 kb on + strand at 16.770 kb on + strand at 16.770 kb on + strand at 16.771 kb on - strand at 17.100 kb on - strand at 17.100 kb on - strand at 17.312 kb on - strand at 17.842 kb on + strand, within RS_RS17260 at 17.842 kb on + strand, within RS_RS17260 at 17.842 kb on + strand, within RS_RS17260 at 17.843 kb on - strand, within RS_RS17260 at 17.843 kb on - strand, within RS_RS17260
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 13,210 + RS_RS17245 0.69 -0.8 13,211 - RS_RS17245 0.69 -0.5 13,211 - RS_RS17245 0.69 -0.4 13,273 + RS_RS17245 0.77 -0.4 13,273 + RS_RS17245 0.77 -0.1 13,486 - +0.0 13,570 + +0.9 13,571 - +0.0 13,575 + -1.4 13,575 + -0.1 13,575 + -0.1 13,576 - -0.6 13,576 - -0.3 13,576 - +0.1 13,576 - -0.2 13,576 - -1.3 13,576 - -0.6 13,576 - -0.1 13,577 + -0.3 13,578 - -0.7 13,730 + RS_RS17250 0.24 -0.3 13,730 + RS_RS17250 0.24 -0.5 13,731 - RS_RS17250 0.25 -1.5 13,931 + RS_RS17250 0.71 -1.2 13,931 + RS_RS17250 0.71 -0.2 13,931 + RS_RS17250 0.71 -0.8 13,932 - RS_RS17250 0.71 +0.9 13,932 - RS_RS17250 0.71 -0.3 13,932 - RS_RS17250 0.71 -1.0 13,932 - RS_RS17250 0.71 +1.6 14,036 + -0.8 14,036 + -0.2 14,036 + +0.6 14,037 - +0.0 14,334 + -0.3 14,334 + +0.2 14,334 + +0.1 14,334 + +0.3 14,334 + -1.2 14,334 + -0.3 14,335 - +0.0 14,335 - +0.2 14,335 - -0.3 14,496 + RS_RS17255 0.13 -0.3 14,682 + RS_RS17255 0.20 -0.5 14,683 - RS_RS17255 0.20 -0.4 14,749 - RS_RS17255 0.22 -0.6 14,749 - RS_RS17255 0.22 -1.0 14,749 - RS_RS17255 0.22 -0.2 15,285 + RS_RS17255 0.42 -0.5 15,285 + RS_RS17255 0.42 -0.7 15,286 - RS_RS17255 0.42 -0.2 15,648 + RS_RS17255 0.56 +0.4 15,648 + RS_RS17255 0.56 -0.5 15,648 + RS_RS17255 0.56 -0.7 15,648 + RS_RS17255 0.56 -0.5 15,649 - RS_RS17255 0.56 -0.1 15,649 - RS_RS17255 0.56 -0.4 15,649 - RS_RS17255 0.56 +1.0 15,650 + RS_RS17255 0.56 -0.3 15,650 + RS_RS17255 0.56 +0.4 15,651 - RS_RS17255 0.56 -0.8 16,119 + RS_RS17255 0.73 +0.3 16,470 - RS_RS17255 0.86 +1.6 16,770 + -0.9 16,770 + -0.7 16,770 + +0.1 16,771 - -0.4 17,100 - -0.8 17,100 - -1.3 17,312 - -1.7 17,842 + RS_RS17260 0.44 +0.5 17,842 + RS_RS17260 0.44 -0.3 17,842 + RS_RS17260 0.44 -1.0 17,843 - RS_RS17260 0.44 +0.3 17,843 - RS_RS17260 0.44 -0.6
Or see this region's nucleotide sequence