Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14015

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14010 and RS_RS14015 are separated by 26 nucleotidesRS_RS14015 and RS_RS14020 are separated by 105 nucleotides RS_RS14010: RS_RS14010 - EamA family transporter, at 3,012,073 to 3,012,909 _RS14010 RS_RS14015: RS_RS14015 - DUF2817 domain-containing protein, at 3,012,936 to 3,014,018 _RS14015 RS_RS14020: RS_RS14020 - ribonucleotide-diphosphate reductase subunit beta, at 3,014,124 to 3,015,326 _RS14020 Position (kb) 3012 3013 3014 3015Strain fitness (log2 ratio) -1 0 1 2at 3011.948 kb on + strandat 3012.263 kb on - strand, within RS_RS14010at 3012.487 kb on + strand, within RS_RS14010at 3012.520 kb on + strand, within RS_RS14010at 3012.520 kb on + strand, within RS_RS14010at 3012.520 kb on + strand, within RS_RS14010at 3012.521 kb on - strand, within RS_RS14010at 3012.522 kb on + strand, within RS_RS14010at 3012.522 kb on + strand, within RS_RS14010at 3012.523 kb on - strand, within RS_RS14010at 3012.989 kb on - strandat 3013.021 kb on + strandat 3013.155 kb on - strand, within RS_RS14015at 3013.447 kb on + strand, within RS_RS14015at 3013.526 kb on - strand, within RS_RS14015at 3013.568 kb on + strand, within RS_RS14015at 3013.598 kb on + strand, within RS_RS14015at 3013.599 kb on - strand, within RS_RS14015at 3013.987 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,011,948 + -0.4
3,012,263 - RS_RS14010 0.23 +0.3
3,012,487 + RS_RS14010 0.49 +0.0
3,012,520 + RS_RS14010 0.53 +0.4
3,012,520 + RS_RS14010 0.53 +1.9
3,012,520 + RS_RS14010 0.53 -1.3
3,012,521 - RS_RS14010 0.54 -0.4
3,012,522 + RS_RS14010 0.54 -0.1
3,012,522 + RS_RS14010 0.54 -0.5
3,012,523 - RS_RS14010 0.54 +0.4
3,012,989 - +0.5
3,013,021 + +0.5
3,013,155 - RS_RS14015 0.20 -0.3
3,013,447 + RS_RS14015 0.47 -0.5
3,013,526 - RS_RS14015 0.54 -0.1
3,013,568 + RS_RS14015 0.58 +0.9
3,013,598 + RS_RS14015 0.61 -1.3
3,013,599 - RS_RS14015 0.61 -0.3
3,013,987 + -0.5

Or see this region's nucleotide sequence