Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS12915 and RS_RS12920 overlap by 4 nucleotides RS_RS12920 and RS_RS12925 are separated by 30 nucleotides
RS_RS12915: RS_RS12915 - P-type conjugative transfer protein TrbG, at 2,783,192 to 2,784,196
_RS12915
RS_RS12920: RS_RS12920 - conjugal transfer protein TrbF, at 2,784,193 to 2,784,897
_RS12920
RS_RS12925: RS_RS12925 - P-type conjugative transfer protein TrbL, at 2,784,928 to 2,786,301
_RS12925
Position (kb)
2784
2785 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2783.288 kb on + strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.289 kb on - strand at 2783.295 kb on + strand, within RS_RS12915 at 2783.295 kb on + strand, within RS_RS12915 at 2783.295 kb on + strand, within RS_RS12915 at 2783.295 kb on + strand, within RS_RS12915 at 2783.295 kb on + strand, within RS_RS12915 at 2783.317 kb on - strand, within RS_RS12915 at 2783.403 kb on + strand, within RS_RS12915 at 2783.404 kb on - strand, within RS_RS12915 at 2783.404 kb on - strand, within RS_RS12915 at 2783.404 kb on - strand, within RS_RS12915 at 2783.404 kb on - strand, within RS_RS12915 at 2783.628 kb on + strand, within RS_RS12915 at 2783.629 kb on - strand, within RS_RS12915 at 2783.629 kb on - strand, within RS_RS12915 at 2783.629 kb on - strand, within RS_RS12915 at 2783.680 kb on + strand, within RS_RS12915 at 2783.830 kb on - strand, within RS_RS12915 at 2783.838 kb on + strand, within RS_RS12915 at 2783.839 kb on - strand, within RS_RS12915 at 2783.883 kb on + strand, within RS_RS12915 at 2783.883 kb on + strand, within RS_RS12915 at 2783.884 kb on - strand, within RS_RS12915 at 2784.174 kb on + strand at 2784.174 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.251 kb on + strand at 2784.252 kb on - strand at 2784.252 kb on - strand at 2784.256 kb on - strand at 2784.256 kb on - strand at 2784.512 kb on + strand, within RS_RS12920 at 2784.512 kb on + strand, within RS_RS12920 at 2784.518 kb on + strand, within RS_RS12920 at 2784.519 kb on - strand, within RS_RS12920 at 2784.519 kb on - strand, within RS_RS12920 at 2784.686 kb on + strand, within RS_RS12920 at 2784.686 kb on + strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.687 kb on - strand, within RS_RS12920 at 2784.839 kb on + strand at 2784.840 kb on - strand at 2784.840 kb on - strand at 2784.922 kb on - strand at 2785.808 kb on - strand, within RS_RS12925 at 2785.808 kb on - strand, within RS_RS12925
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,783,288 + +0.1 2,783,289 - +0.4 2,783,289 - -0.6 2,783,289 - -1.0 2,783,289 - -0.2 2,783,289 - -0.3 2,783,289 - +0.3 2,783,289 - -0.1 2,783,295 + RS_RS12915 0.10 -0.6 2,783,295 + RS_RS12915 0.10 -0.4 2,783,295 + RS_RS12915 0.10 +0.8 2,783,295 + RS_RS12915 0.10 +0.8 2,783,295 + RS_RS12915 0.10 +0.1 2,783,317 - RS_RS12915 0.12 -0.3 2,783,403 + RS_RS12915 0.21 +0.1 2,783,404 - RS_RS12915 0.21 -0.5 2,783,404 - RS_RS12915 0.21 -0.0 2,783,404 - RS_RS12915 0.21 -0.1 2,783,404 - RS_RS12915 0.21 -1.1 2,783,628 + RS_RS12915 0.43 -0.1 2,783,629 - RS_RS12915 0.43 +0.0 2,783,629 - RS_RS12915 0.43 -0.5 2,783,629 - RS_RS12915 0.43 +0.1 2,783,680 + RS_RS12915 0.49 -0.1 2,783,830 - RS_RS12915 0.63 +0.0 2,783,838 + RS_RS12915 0.64 +0.4 2,783,839 - RS_RS12915 0.64 -0.1 2,783,883 + RS_RS12915 0.69 +0.8 2,783,883 + RS_RS12915 0.69 -0.1 2,783,884 - RS_RS12915 0.69 +0.2 2,784,174 + +0.3 2,784,174 + -1.5 2,784,251 + +0.4 2,784,251 + -0.3 2,784,251 + -0.0 2,784,251 + -0.7 2,784,251 + +0.4 2,784,252 - -3.2 2,784,252 - -2.5 2,784,256 - -4.4 2,784,256 - -5.2 2,784,512 + RS_RS12920 0.45 -0.1 2,784,512 + RS_RS12920 0.45 -0.2 2,784,518 + RS_RS12920 0.46 +0.8 2,784,519 - RS_RS12920 0.46 -1.6 2,784,519 - RS_RS12920 0.46 -4.7 2,784,686 + RS_RS12920 0.70 +1.8 2,784,686 + RS_RS12920 0.70 +0.0 2,784,687 - RS_RS12920 0.70 -3.0 2,784,687 - RS_RS12920 0.70 -4.0 2,784,687 - RS_RS12920 0.70 -2.5 2,784,839 + -1.9 2,784,840 - -1.2 2,784,840 - -2.5 2,784,922 - -1.7 2,785,808 - RS_RS12925 0.64 -2.8 2,785,808 - RS_RS12925 0.64 -6.1
Or see this region's nucleotide sequence