Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS06915 and RS_RS06920 are separated by 34 nucleotides RS_RS06920 and RS_RS06925 are separated by 64 nucleotides RS_RS06925 and RS_RS06930 are separated by 10 nucleotides
RS_RS06915: RS_RS06915 - ABC transporter ATP-binding protein, at 1,469,583 to 1,471,466
_RS06915
RS_RS06920: RS_RS06920 - glutathione ABC transporter substrate-binding protein GsiB, at 1,471,501 to 1,473,054
_RS06920
RS_RS06925: RS_RS06925 - glutathione ABC transporter permease GsiC, at 1,473,119 to 1,474,042
_RS06925
RS_RS06930: RS_RS06930 - glutathione ABC transporter permease GsiD, at 1,474,053 to 1,474,952
_RS06930
Position (kb) 1471
1472
1473
1474 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1470.645 kb on + strand, within RS_RS06915 at 1470.646 kb on - strand, within RS_RS06915 at 1471.215 kb on + strand, within RS_RS06915 at 1471.216 kb on - strand, within RS_RS06915 at 1471.216 kb on - strand, within RS_RS06915 at 1471.216 kb on - strand, within RS_RS06915 at 1471.278 kb on + strand at 1471.279 kb on - strand at 1471.462 kb on - strand at 1471.639 kb on + strand at 1471.685 kb on - strand, within RS_RS06920 at 1471.744 kb on + strand, within RS_RS06920 at 1471.744 kb on + strand, within RS_RS06920 at 1471.744 kb on + strand, within RS_RS06920 at 1471.745 kb on - strand, within RS_RS06920 at 1471.745 kb on - strand, within RS_RS06920 at 1471.745 kb on - strand, within RS_RS06920 at 1471.885 kb on + strand, within RS_RS06920 at 1471.930 kb on + strand, within RS_RS06920 at 1472.030 kb on - strand, within RS_RS06920 at 1472.030 kb on - strand, within RS_RS06920 at 1472.317 kb on + strand, within RS_RS06920 at 1472.318 kb on - strand, within RS_RS06920 at 1472.380 kb on + strand, within RS_RS06920 at 1472.381 kb on - strand, within RS_RS06920 at 1472.468 kb on - strand, within RS_RS06920 at 1472.495 kb on - strand, within RS_RS06920 at 1472.574 kb on + strand, within RS_RS06920 at 1472.725 kb on + strand, within RS_RS06920 at 1472.728 kb on + strand, within RS_RS06920 at 1472.728 kb on + strand, within RS_RS06920 at 1472.728 kb on + strand, within RS_RS06920 at 1472.728 kb on + strand, within RS_RS06920 at 1472.729 kb on - strand, within RS_RS06920 at 1472.729 kb on - strand, within RS_RS06920 at 1472.729 kb on - strand, within RS_RS06920 at 1472.827 kb on + strand, within RS_RS06920 at 1472.828 kb on - strand, within RS_RS06920 at 1472.971 kb on + strand at 1473.001 kb on + strand at 1473.128 kb on + strand at 1473.635 kb on + strand, within RS_RS06925 at 1473.635 kb on + strand, within RS_RS06925 at 1473.635 kb on + strand, within RS_RS06925 at 1473.636 kb on - strand, within RS_RS06925 at 1473.822 kb on - strand, within RS_RS06925 at 1474.005 kb on - strand at 1474.040 kb on + strand at 1474.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,470,645 + RS_RS06915 0.56 -0.7 1,470,646 - RS_RS06915 0.56 -1.9 1,471,215 + RS_RS06915 0.87 +0.7 1,471,216 - RS_RS06915 0.87 -0.2 1,471,216 - RS_RS06915 0.87 -0.7 1,471,216 - RS_RS06915 0.87 +2.6 1,471,278 + +1.0 1,471,279 - +0.6 1,471,462 - +0.6 1,471,639 + -0.6 1,471,685 - RS_RS06920 0.12 -0.0 1,471,744 + RS_RS06920 0.16 +1.6 1,471,744 + RS_RS06920 0.16 +0.3 1,471,744 + RS_RS06920 0.16 -0.0 1,471,745 - RS_RS06920 0.16 -1.2 1,471,745 - RS_RS06920 0.16 -0.7 1,471,745 - RS_RS06920 0.16 +0.1 1,471,885 + RS_RS06920 0.25 +0.5 1,471,930 + RS_RS06920 0.28 -0.4 1,472,030 - RS_RS06920 0.34 -1.2 1,472,030 - RS_RS06920 0.34 -1.2 1,472,317 + RS_RS06920 0.53 +0.6 1,472,318 - RS_RS06920 0.53 +0.7 1,472,380 + RS_RS06920 0.57 +0.3 1,472,381 - RS_RS06920 0.57 -0.0 1,472,468 - RS_RS06920 0.62 +0.4 1,472,495 - RS_RS06920 0.64 +1.3 1,472,574 + RS_RS06920 0.69 +2.0 1,472,725 + RS_RS06920 0.79 +1.6 1,472,728 + RS_RS06920 0.79 -0.2 1,472,728 + RS_RS06920 0.79 -0.6 1,472,728 + RS_RS06920 0.79 +2.4 1,472,728 + RS_RS06920 0.79 +1.6 1,472,729 - RS_RS06920 0.79 -0.7 1,472,729 - RS_RS06920 0.79 +0.6 1,472,729 - RS_RS06920 0.79 +0.6 1,472,827 + RS_RS06920 0.85 +0.2 1,472,828 - RS_RS06920 0.85 -0.2 1,472,971 + +0.7 1,473,001 + +0.5 1,473,128 + +0.4 1,473,635 + RS_RS06925 0.56 +0.6 1,473,635 + RS_RS06925 0.56 +1.6 1,473,635 + RS_RS06925 0.56 +0.6 1,473,636 - RS_RS06925 0.56 +0.3 1,473,822 - RS_RS06925 0.76 +0.2 1,474,005 - -1.0 1,474,040 + +0.1 1,474,040 + +0.2
Or see this region's nucleotide sequence