Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16760 and RS_RS16765 are separated by 64 nucleotides RS_RS16765 and RS_RS16770 are separated by 56 nucleotides RS_RS16770 and RS_RS16775 overlap by 4 nucleotides
RS_RS16760: RS_RS16760 - choline dehydrogenase, at 3,605,487 to 3,607,121
_RS16760
RS_RS16765: RS_RS16765 - alpha/beta hydrolase, at 3,607,186 to 3,608,046
_RS16765
RS_RS16770: RS_RS16770 - Cu(I)-responsive transcriptional regulator, at 3,608,103 to 3,608,582
_RS16770
RS_RS16775: RS_RS16775 - copper-translocating P-type ATPase, at 3,608,579 to 3,610,825
_RS16775
Position (kb)
3607
3608
3609 Strain fitness (log2 ratio)
-1
0
1 at 3606.192 kb on - strand, within RS_RS16760 at 3606.192 kb on - strand, within RS_RS16760 at 3606.400 kb on + strand, within RS_RS16760 at 3606.401 kb on - strand, within RS_RS16760 at 3606.421 kb on + strand, within RS_RS16760 at 3606.572 kb on - strand, within RS_RS16760 at 3606.733 kb on + strand, within RS_RS16760 at 3606.733 kb on + strand, within RS_RS16760 at 3606.734 kb on - strand, within RS_RS16760 at 3606.734 kb on - strand, within RS_RS16760 at 3606.734 kb on - strand, within RS_RS16760 at 3606.814 kb on + strand, within RS_RS16760 at 3606.838 kb on + strand, within RS_RS16760 at 3606.838 kb on + strand, within RS_RS16760 at 3606.838 kb on + strand, within RS_RS16760 at 3606.839 kb on - strand, within RS_RS16760 at 3606.839 kb on - strand, within RS_RS16760 at 3606.839 kb on - strand, within RS_RS16760 at 3606.839 kb on - strand, within RS_RS16760 at 3606.967 kb on + strand at 3606.967 kb on + strand at 3606.967 kb on + strand at 3606.967 kb on + strand at 3606.968 kb on - strand at 3606.974 kb on - strand at 3607.000 kb on + strand at 3607.000 kb on + strand at 3607.001 kb on - strand at 3607.001 kb on - strand at 3607.105 kb on + strand at 3607.105 kb on + strand at 3607.105 kb on + strand at 3607.105 kb on + strand at 3607.105 kb on + strand at 3607.105 kb on + strand at 3607.106 kb on - strand at 3607.106 kb on - strand at 3607.106 kb on - strand at 3607.106 kb on - strand at 3607.111 kb on + strand at 3607.111 kb on + strand at 3607.111 kb on + strand at 3607.111 kb on + strand at 3607.112 kb on - strand at 3607.268 kb on + strand at 3607.269 kb on - strand at 3607.269 kb on - strand at 3607.772 kb on + strand, within RS_RS16765 at 3607.772 kb on + strand, within RS_RS16765 at 3607.772 kb on + strand, within RS_RS16765 at 3607.773 kb on - strand, within RS_RS16765 at 3608.081 kb on + strand at 3608.426 kb on - strand, within RS_RS16770 at 3608.426 kb on - strand, within RS_RS16770 at 3608.482 kb on + strand, within RS_RS16770 at 3608.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 3,606,192 - RS_RS16760 0.43 +0.3 3,606,192 - RS_RS16760 0.43 -0.5 3,606,400 + RS_RS16760 0.56 -0.4 3,606,401 - RS_RS16760 0.56 +0.3 3,606,421 + RS_RS16760 0.57 -0.2 3,606,572 - RS_RS16760 0.66 -0.2 3,606,733 + RS_RS16760 0.76 +0.1 3,606,733 + RS_RS16760 0.76 -0.0 3,606,734 - RS_RS16760 0.76 -0.4 3,606,734 - RS_RS16760 0.76 +0.1 3,606,734 - RS_RS16760 0.76 -0.3 3,606,814 + RS_RS16760 0.81 -0.4 3,606,838 + RS_RS16760 0.83 -0.3 3,606,838 + RS_RS16760 0.83 -0.2 3,606,838 + RS_RS16760 0.83 -0.7 3,606,839 - RS_RS16760 0.83 -0.7 3,606,839 - RS_RS16760 0.83 +0.3 3,606,839 - RS_RS16760 0.83 +0.2 3,606,839 - RS_RS16760 0.83 -0.1 3,606,967 + +0.2 3,606,967 + -0.1 3,606,967 + -0.6 3,606,967 + -0.2 3,606,968 - +0.1 3,606,974 - -0.2 3,607,000 + -0.1 3,607,000 + +0.0 3,607,001 - +0.3 3,607,001 - -0.1 3,607,105 + +0.4 3,607,105 + -0.1 3,607,105 + -0.7 3,607,105 + +0.2 3,607,105 + +1.3 3,607,105 + +0.0 3,607,106 - -0.1 3,607,106 - +0.5 3,607,106 - -1.4 3,607,106 - +0.3 3,607,111 + -0.1 3,607,111 + -0.2 3,607,111 + -0.1 3,607,111 + -0.1 3,607,112 - +0.1 3,607,268 + +0.5 3,607,269 - -0.2 3,607,269 - +0.1 3,607,772 + RS_RS16765 0.68 -1.2 3,607,772 + RS_RS16765 0.68 -1.3 3,607,772 + RS_RS16765 0.68 +0.0 3,607,773 - RS_RS16765 0.68 +0.0 3,608,081 + +0.2 3,608,426 - RS_RS16770 0.67 -0.3 3,608,426 - RS_RS16770 0.67 +0.1 3,608,482 + RS_RS16770 0.79 +0.3 3,608,743 - +0.0
Or see this region's nucleotide sequence