Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS14815 and RS_RS14820 are separated by 234 nucleotides RS_RS14820 and RS_RS14825 are separated by 66 nucleotides RS_RS14825 and RS_RS14830 are separated by 154 nucleotides RS_RS14830 and RS_RS14835 are separated by 3 nucleotides
RS_RS14815: RS_RS14815 - ABC transporter ATP-binding protein, at 3,184,945 to 3,185,910
_RS14815
RS_RS14820: RS_RS14820 - STAS domain-containing protein, at 3,186,145 to 3,186,459
_RS14820
RS_RS14825: RS_RS14825 - hypothetical protein, at 3,186,526 to 3,187,161
_RS14825
RS_RS14830: RS_RS14830 - ABC transporter, at 3,187,316 to 3,188,125
_RS14830
RS_RS14835: RS_RS14835 - outer membrane lipid asymmetry maintenance protein MlaD, at 3,188,129 to 3,188,650
_RS14835
Position (kb)
3186
3187
3188 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3185.604 kb on - strand, within RS_RS14815 at 3185.874 kb on - strand at 3185.921 kb on - strand at 3186.213 kb on - strand, within RS_RS14820 at 3186.671 kb on + strand, within RS_RS14825 at 3186.671 kb on + strand, within RS_RS14825 at 3186.672 kb on - strand, within RS_RS14825 at 3186.794 kb on + strand, within RS_RS14825 at 3186.836 kb on + strand, within RS_RS14825 at 3187.273 kb on + strand at 3187.303 kb on + strand at 3187.303 kb on + strand at 3187.356 kb on + strand at 3187.461 kb on + strand, within RS_RS14830 at 3187.482 kb on + strand, within RS_RS14830 at 3187.483 kb on - strand, within RS_RS14830 at 3187.570 kb on - strand, within RS_RS14830 at 3187.593 kb on + strand, within RS_RS14830 at 3187.678 kb on - strand, within RS_RS14830 at 3187.678 kb on - strand, within RS_RS14830 at 3187.678 kb on - strand, within RS_RS14830 at 3187.932 kb on + strand, within RS_RS14830 at 3187.932 kb on + strand, within RS_RS14830 at 3187.932 kb on + strand, within RS_RS14830 at 3187.932 kb on + strand, within RS_RS14830 at 3187.933 kb on - strand, within RS_RS14830 at 3188.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 3,185,604 - RS_RS14815 0.68 +1.3 3,185,874 - +0.3 3,185,921 - -0.5 3,186,213 - RS_RS14820 0.22 -3.9 3,186,671 + RS_RS14825 0.23 -5.3 3,186,671 + RS_RS14825 0.23 -4.9 3,186,672 - RS_RS14825 0.23 -4.7 3,186,794 + RS_RS14825 0.42 -4.0 3,186,836 + RS_RS14825 0.49 -4.7 3,187,273 + +0.1 3,187,303 + +0.1 3,187,303 + -0.1 3,187,356 + -0.0 3,187,461 + RS_RS14830 0.18 -4.7 3,187,482 + RS_RS14830 0.20 -4.9 3,187,483 - RS_RS14830 0.21 -3.8 3,187,570 - RS_RS14830 0.31 -5.3 3,187,593 + RS_RS14830 0.34 -5.4 3,187,678 - RS_RS14830 0.45 -5.3 3,187,678 - RS_RS14830 0.45 -5.3 3,187,678 - RS_RS14830 0.45 -5.4 3,187,932 + RS_RS14830 0.76 -4.2 3,187,932 + RS_RS14830 0.76 -5.8 3,187,932 + RS_RS14830 0.76 -5.4 3,187,932 + RS_RS14830 0.76 -5.3 3,187,933 - RS_RS14830 0.76 -5.8 3,188,130 + -1.0
Or see this region's nucleotide sequence