Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14825

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14815 and RS_RS14820 are separated by 234 nucleotidesRS_RS14820 and RS_RS14825 are separated by 66 nucleotidesRS_RS14825 and RS_RS14830 are separated by 154 nucleotidesRS_RS14830 and RS_RS14835 are separated by 3 nucleotides RS_RS14815: RS_RS14815 - ABC transporter ATP-binding protein, at 3,184,945 to 3,185,910 _RS14815 RS_RS14820: RS_RS14820 - STAS domain-containing protein, at 3,186,145 to 3,186,459 _RS14820 RS_RS14825: RS_RS14825 - hypothetical protein, at 3,186,526 to 3,187,161 _RS14825 RS_RS14830: RS_RS14830 - ABC transporter, at 3,187,316 to 3,188,125 _RS14830 RS_RS14835: RS_RS14835 - outer membrane lipid asymmetry maintenance protein MlaD, at 3,188,129 to 3,188,650 _RS14835 Position (kb) 3186 3187 3188Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3185.604 kb on - strand, within RS_RS14815at 3185.874 kb on - strandat 3185.921 kb on - strandat 3186.213 kb on - strand, within RS_RS14820at 3186.671 kb on + strand, within RS_RS14825at 3186.671 kb on + strand, within RS_RS14825at 3186.672 kb on - strand, within RS_RS14825at 3186.794 kb on + strand, within RS_RS14825at 3186.836 kb on + strand, within RS_RS14825at 3187.273 kb on + strandat 3187.303 kb on + strandat 3187.303 kb on + strandat 3187.356 kb on + strandat 3187.461 kb on + strand, within RS_RS14830at 3187.482 kb on + strand, within RS_RS14830at 3187.483 kb on - strand, within RS_RS14830at 3187.570 kb on - strand, within RS_RS14830at 3187.593 kb on + strand, within RS_RS14830at 3187.678 kb on - strand, within RS_RS14830at 3187.678 kb on - strand, within RS_RS14830at 3187.678 kb on - strand, within RS_RS14830at 3187.932 kb on + strand, within RS_RS14830at 3187.932 kb on + strand, within RS_RS14830at 3187.932 kb on + strand, within RS_RS14830at 3187.932 kb on + strand, within RS_RS14830at 3187.933 kb on - strand, within RS_RS14830at 3188.130 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
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3,185,604 - RS_RS14815 0.68 +1.3
3,185,874 - +0.3
3,185,921 - -0.5
3,186,213 - RS_RS14820 0.22 -3.9
3,186,671 + RS_RS14825 0.23 -5.3
3,186,671 + RS_RS14825 0.23 -4.9
3,186,672 - RS_RS14825 0.23 -4.7
3,186,794 + RS_RS14825 0.42 -4.0
3,186,836 + RS_RS14825 0.49 -4.7
3,187,273 + +0.1
3,187,303 + +0.1
3,187,303 + -0.1
3,187,356 + -0.0
3,187,461 + RS_RS14830 0.18 -4.7
3,187,482 + RS_RS14830 0.20 -4.9
3,187,483 - RS_RS14830 0.21 -3.8
3,187,570 - RS_RS14830 0.31 -5.3
3,187,593 + RS_RS14830 0.34 -5.4
3,187,678 - RS_RS14830 0.45 -5.3
3,187,678 - RS_RS14830 0.45 -5.3
3,187,678 - RS_RS14830 0.45 -5.4
3,187,932 + RS_RS14830 0.76 -4.2
3,187,932 + RS_RS14830 0.76 -5.8
3,187,932 + RS_RS14830 0.76 -5.4
3,187,932 + RS_RS14830 0.76 -5.3
3,187,933 - RS_RS14830 0.76 -5.8
3,188,130 + -1.0

Or see this region's nucleotide sequence