Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS11165 and RS_RS11170 are separated by 97 nucleotides RS_RS11170 and RS_RS11175 are separated by 29 nucleotides RS_RS11175 and RS_RS11180 are separated by 76 nucleotides
RS_RS11165: RS_RS11165 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 2,411,998 to 2,413,095
_RS11165
RS_RS11170: RS_RS11170 - EamA/RhaT family transporter, at 2,413,193 to 2,414,167
_RS11170
RS_RS11175: RS_RS11175 - sulfurtransferase, at 2,414,197 to 2,415,087
_RS11175
RS_RS11180: RS_RS11180 - ZIP family metal transporter, at 2,415,164 to 2,415,970
_RS11180
Position (kb)
2413
2414
2415 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2412.236 kb on + strand, within RS_RS11165 at 2412.236 kb on + strand, within RS_RS11165 at 2412.237 kb on - strand, within RS_RS11165 at 2412.316 kb on + strand, within RS_RS11165 at 2412.316 kb on + strand, within RS_RS11165 at 2412.316 kb on + strand, within RS_RS11165 at 2412.317 kb on - strand, within RS_RS11165 at 2412.488 kb on - strand, within RS_RS11165 at 2412.562 kb on + strand, within RS_RS11165 at 2412.563 kb on - strand, within RS_RS11165 at 2412.563 kb on - strand, within RS_RS11165 at 2412.563 kb on - strand, within RS_RS11165 at 2412.640 kb on + strand, within RS_RS11165 at 2412.697 kb on + strand, within RS_RS11165 at 2412.698 kb on - strand, within RS_RS11165 at 2412.772 kb on + strand, within RS_RS11165 at 2412.773 kb on - strand, within RS_RS11165 at 2412.872 kb on - strand, within RS_RS11165 at 2412.875 kb on - strand, within RS_RS11165 at 2412.932 kb on - strand, within RS_RS11165 at 2413.090 kb on + strand at 2413.090 kb on + strand at 2413.207 kb on + strand at 2413.208 kb on - strand at 2413.347 kb on - strand, within RS_RS11170 at 2413.388 kb on + strand, within RS_RS11170 at 2413.523 kb on + strand, within RS_RS11170 at 2413.523 kb on + strand, within RS_RS11170 at 2413.524 kb on - strand, within RS_RS11170 at 2413.524 kb on - strand, within RS_RS11170 at 2413.524 kb on - strand, within RS_RS11170 at 2413.524 kb on - strand, within RS_RS11170 at 2413.524 kb on - strand, within RS_RS11170 at 2413.727 kb on + strand, within RS_RS11170 at 2413.784 kb on + strand, within RS_RS11170 at 2413.926 kb on - strand, within RS_RS11170 at 2415.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 2,412,236 + RS_RS11165 0.22 -0.5 2,412,236 + RS_RS11165 0.22 -4.6 2,412,237 - RS_RS11165 0.22 -1.2 2,412,316 + RS_RS11165 0.29 -2.7 2,412,316 + RS_RS11165 0.29 -3.1 2,412,316 + RS_RS11165 0.29 -3.6 2,412,317 - RS_RS11165 0.29 -3.3 2,412,488 - RS_RS11165 0.45 -4.0 2,412,562 + RS_RS11165 0.51 -4.6 2,412,563 - RS_RS11165 0.51 -1.7 2,412,563 - RS_RS11165 0.51 -3.9 2,412,563 - RS_RS11165 0.51 -4.3 2,412,640 + RS_RS11165 0.58 -3.5 2,412,697 + RS_RS11165 0.64 -4.1 2,412,698 - RS_RS11165 0.64 -3.8 2,412,772 + RS_RS11165 0.70 -3.6 2,412,773 - RS_RS11165 0.71 -3.5 2,412,872 - RS_RS11165 0.80 -2.2 2,412,875 - RS_RS11165 0.80 -4.2 2,412,932 - RS_RS11165 0.85 -4.0 2,413,090 + +1.1 2,413,090 + -0.4 2,413,207 + -0.9 2,413,208 - -1.5 2,413,347 - RS_RS11170 0.16 -0.2 2,413,388 + RS_RS11170 0.20 -0.4 2,413,523 + RS_RS11170 0.34 +0.3 2,413,523 + RS_RS11170 0.34 +0.0 2,413,524 - RS_RS11170 0.34 +0.1 2,413,524 - RS_RS11170 0.34 -0.5 2,413,524 - RS_RS11170 0.34 +0.3 2,413,524 - RS_RS11170 0.34 -0.6 2,413,524 - RS_RS11170 0.34 -0.2 2,413,727 + RS_RS11170 0.55 +0.1 2,413,784 + RS_RS11170 0.61 -0.2 2,413,926 - RS_RS11170 0.75 -0.2 2,415,020 - -0.4
Or see this region's nucleotide sequence