Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS09010 and RS_RS09015 are separated by 60 nucleotides RS_RS09015 and RS_RS09020 are separated by 52 nucleotides RS_RS09020 and RS_RS09025 overlap by 17 nucleotides
RS_RS09010: RS_RS09010 - DNA polymerase III subunit delta', at 1,920,565 to 1,921,575
_RS09010
RS_RS09015: RS_RS09015 - type 4 fimbrial biogenesis protein, at 1,921,636 to 1,922,025
_RS09015
RS_RS09020: RS_RS09020 - TatD family deoxyribonuclease, at 1,922,078 to 1,922,881
_RS09020
RS_RS09025: RS_RS09025 - hypothetical protein, at 1,922,865 to 1,923,740
_RS09025
Position (kb) 1921
1922
1923 Strain fitness (log2 ratio) -1
0
1 at 1921.611 kb on + strand at 1921.738 kb on + strand, within RS_RS09015 at 1921.738 kb on + strand, within RS_RS09015 at 1921.748 kb on - strand, within RS_RS09015 at 1921.748 kb on - strand, within RS_RS09015 at 1922.061 kb on - strand at 1922.246 kb on + strand, within RS_RS09020 at 1922.246 kb on + strand, within RS_RS09020 at 1922.247 kb on - strand, within RS_RS09020 at 1922.385 kb on - strand, within RS_RS09020 at 1922.387 kb on + strand, within RS_RS09020 at 1922.619 kb on - strand, within RS_RS09020 at 1922.619 kb on - strand, within RS_RS09020 at 1922.723 kb on + strand, within RS_RS09020 at 1922.768 kb on + strand, within RS_RS09020 at 1922.769 kb on - strand, within RS_RS09020 at 1922.879 kb on + strand at 1922.988 kb on + strand, within RS_RS09025 at 1922.988 kb on + strand, within RS_RS09025 at 1922.989 kb on - strand, within RS_RS09025
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,921,611 + +0.1 1,921,738 + RS_RS09015 0.26 -0.4 1,921,738 + RS_RS09015 0.26 +0.1 1,921,748 - RS_RS09015 0.29 -1.4 1,921,748 - RS_RS09015 0.29 +1.0 1,922,061 - -0.2 1,922,246 + RS_RS09020 0.21 -1.3 1,922,246 + RS_RS09020 0.21 -0.1 1,922,247 - RS_RS09020 0.21 -0.8 1,922,385 - RS_RS09020 0.38 -0.6 1,922,387 + RS_RS09020 0.38 +0.5 1,922,619 - RS_RS09020 0.67 -1.0 1,922,619 - RS_RS09020 0.67 -1.2 1,922,723 + RS_RS09020 0.80 -0.3 1,922,768 + RS_RS09020 0.86 -1.2 1,922,769 - RS_RS09020 0.86 +0.4 1,922,879 + +0.0 1,922,988 + RS_RS09025 0.14 -1.0 1,922,988 + RS_RS09025 0.14 -0.6 1,922,989 - RS_RS09025 0.14 -0.4
Or see this region's nucleotide sequence