Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS03590 and RS_RS03595 are separated by 103 nucleotides RS_RS03595 and RS_RS03600 are separated by 8 nucleotides RS_RS03600 and RS_RS03605 are separated by 28 nucleotides RS_RS03605 and RS_RS03610 overlap by 4 nucleotides
RS_RS03590: RS_RS03590 - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 760,616 to 761,746
_RS03590
RS_RS03595: RS_RS03595 - riboflavin synthase, at 761,850 to 762,503
_RS03595
RS_RS03600: RS_RS03600 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 762,512 to 763,624
_RS03600
RS_RS03605: RS_RS03605 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 763,653 to 764,213
_RS03605
RS_RS03610: RS_RS03610 - type IV pilin protein, at 764,210 to 764,641
_RS03610
Position (kb) 762
763
764 Strain fitness (log2 ratio) -1
0
1 at 761.821 kb on + strand at 761.821 kb on + strand at 761.822 kb on - strand at 763.820 kb on - strand, within RS_RS03605 at 763.820 kb on - strand, within RS_RS03605 at 763.822 kb on + strand, within RS_RS03605 at 763.961 kb on - strand, within RS_RS03605 at 763.963 kb on - strand, within RS_RS03605 at 764.133 kb on + strand, within RS_RS03605 at 764.133 kb on + strand, within RS_RS03605 at 764.134 kb on - strand, within RS_RS03605 at 764.454 kb on + strand, within RS_RS03610 at 764.455 kb on - strand, within RS_RS03610 at 764.455 kb on - strand, within RS_RS03610 at 764.542 kb on - strand, within RS_RS03610 at 764.542 kb on - strand, within RS_RS03610
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 761,821 + -0.7 761,821 + -1.2 761,822 - -0.1 763,820 - RS_RS03605 0.30 -0.0 763,820 - RS_RS03605 0.30 +0.3 763,822 + RS_RS03605 0.30 -0.5 763,961 - RS_RS03605 0.55 -0.4 763,963 - RS_RS03605 0.55 +0.1 764,133 + RS_RS03605 0.86 +0.4 764,133 + RS_RS03605 0.86 -0.1 764,134 - RS_RS03605 0.86 +0.2 764,454 + RS_RS03610 0.56 +0.3 764,455 - RS_RS03610 0.57 -0.1 764,455 - RS_RS03610 0.57 +0.8 764,542 - RS_RS03610 0.77 +0.9 764,542 - RS_RS03610 0.77 +0.8
Or see this region's nucleotide sequence