Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15370 and RS_RS15375 are separated by 512 nucleotides RS_RS15375 and RS_RS15380 are separated by 112 nucleotides
RS_RS15370: RS_RS15370 - MFS transporter, at 3,295,987 to 3,297,282
_RS15370
RS_RS15375: RS_RS15375 - MFS transporter, at 3,297,795 to 3,299,096
_RS15375
RS_RS15380: RS_RS15380 - ethanolamine utilization protein EutH, at 3,299,209 to 3,300,375
_RS15380
Position (kb)
3297
3298
3299
3300 Strain fitness (log2 ratio)
-1
0
1 at 3297.019 kb on + strand, within RS_RS15370 at 3297.019 kb on + strand, within RS_RS15370 at 3297.020 kb on - strand, within RS_RS15370 at 3297.020 kb on - strand, within RS_RS15370 at 3297.095 kb on - strand, within RS_RS15370 at 3297.095 kb on - strand, within RS_RS15370 at 3297.623 kb on - strand at 3297.771 kb on + strand at 3297.771 kb on + strand at 3297.771 kb on + strand at 3297.772 kb on - strand at 3297.801 kb on - strand at 3297.801 kb on - strand at 3297.885 kb on + strand at 3297.891 kb on + strand at 3297.894 kb on + strand at 3297.894 kb on + strand at 3297.894 kb on + strand at 3297.895 kb on - strand at 3297.949 kb on + strand, within RS_RS15375 at 3297.956 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.207 kb on - strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.246 kb on - strand, within RS_RS15375 at 3298.548 kb on + strand, within RS_RS15375 at 3298.549 kb on - strand, within RS_RS15375 at 3298.549 kb on - strand, within RS_RS15375 at 3298.549 kb on - strand, within RS_RS15375 at 3298.903 kb on - strand, within RS_RS15375 at 3299.210 kb on + strand at 3299.255 kb on + strand at 3299.255 kb on + strand at 3299.255 kb on + strand at 3299.256 kb on - strand at 3299.315 kb on + strand at 3299.315 kb on + strand at 3299.316 kb on - strand at 3299.316 kb on - strand at 3299.837 kb on + strand, within RS_RS15380 at 3299.838 kb on - strand, within RS_RS15380 at 3299.838 kb on - strand, within RS_RS15380 at 3299.838 kb on - strand, within RS_RS15380 at 3299.838 kb on - strand, within RS_RS15380
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 3,297,019 + RS_RS15370 0.80 -0.6 3,297,019 + RS_RS15370 0.80 -0.1 3,297,020 - RS_RS15370 0.80 +0.9 3,297,020 - RS_RS15370 0.80 +0.2 3,297,095 - RS_RS15370 0.85 -1.1 3,297,095 - RS_RS15370 0.85 -0.2 3,297,623 - +0.0 3,297,771 + -0.1 3,297,771 + -1.1 3,297,771 + -0.6 3,297,772 - -0.9 3,297,801 - +0.1 3,297,801 - -0.1 3,297,885 + +0.4 3,297,891 + -0.2 3,297,894 + -0.9 3,297,894 + +1.3 3,297,894 + +0.7 3,297,895 - -0.1 3,297,949 + RS_RS15375 0.12 +0.8 3,297,956 - RS_RS15375 0.12 -0.1 3,298,021 - RS_RS15375 0.17 -0.4 3,298,021 - RS_RS15375 0.17 -0.1 3,298,021 - RS_RS15375 0.17 +1.3 3,298,207 - RS_RS15375 0.32 -0.7 3,298,245 + RS_RS15375 0.35 +0.2 3,298,245 + RS_RS15375 0.35 -1.0 3,298,245 + RS_RS15375 0.35 -0.7 3,298,246 - RS_RS15375 0.35 -0.2 3,298,548 + RS_RS15375 0.58 +0.2 3,298,549 - RS_RS15375 0.58 +0.1 3,298,549 - RS_RS15375 0.58 -0.1 3,298,549 - RS_RS15375 0.58 +0.2 3,298,903 - RS_RS15375 0.85 -0.1 3,299,210 + -0.8 3,299,255 + -0.2 3,299,255 + +0.0 3,299,255 + -0.2 3,299,256 - -0.1 3,299,315 + +0.1 3,299,315 + +0.0 3,299,316 - +0.2 3,299,316 - +1.0 3,299,837 + RS_RS15380 0.54 -0.3 3,299,838 - RS_RS15380 0.54 -0.1 3,299,838 - RS_RS15380 0.54 +0.2 3,299,838 - RS_RS15380 0.54 -0.0 3,299,838 - RS_RS15380 0.54 +0.8
Or see this region's nucleotide sequence