Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS13220

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRS_RS13210 and RS_RS13215 are separated by 263 nucleotidesRS_RS13215 and RS_RS13220 are separated by 15 nucleotidesRS_RS13220 and RS_RS25585 are separated by 32 nucleotidesRS_RS25585 and RS_RS13225 are separated by 47 nucleotidesRS_RS13225 and RS_RS13230 are separated by 13 nucleotidesRS_RS13230 and RS_RS13235 overlap by 4 nucleotides RS_RS13210: RS_RS13210 - nucleoside diphosphate kinase regulator, at 2,840,328 to 2,840,732 _RS13210 RS_RS13215: RS_RS13215 - thioredoxin, at 2,840,996 to 2,841,418 _RS13215 RS_RS13220: RS_RS13220 - nucleotide exchange factor GrpE, at 2,841,434 to 2,842,078 _RS13220 RS_RS25585: RS_RS25585 - transposase, at 2,842,111 to 2,842,314 _RS25585 RS_RS13225: RS_RS13225 - hypothetical protein, at 2,842,362 to 2,842,550 _RS13225 RS_RS13230: RS_RS13230 - RNA-binding S4 domain-containing protein, at 2,842,564 to 2,842,959 _RS13230 RS_RS13235: RS_RS13235 - ferrochelatase, at 2,842,956 to 2,844,071 _RS13235 Position (kb) 2841 2842 2843Strain fitness (log2 ratio) -1 0 1at 2840.713 kb on + strandat 2840.713 kb on + strandat 2840.713 kb on + strandat 2840.714 kb on - strandat 2840.714 kb on - strandat 2841.436 kb on - strandat 2842.247 kb on - strand, within RS_RS25585at 2842.254 kb on + strand, within RS_RS25585at 2842.255 kb on - strand, within RS_RS25585at 2842.255 kb on - strand, within RS_RS25585at 2842.310 kb on + strandat 2842.310 kb on + strandat 2842.310 kb on + strandat 2842.310 kb on + strandat 2842.310 kb on + strandat 2842.311 kb on - strandat 2842.311 kb on - strandat 2842.311 kb on - strandat 2842.344 kb on + strandat 2842.345 kb on - strandat 2842.345 kb on - strandat 2842.359 kb on - strandat 2842.377 kb on - strandat 2842.494 kb on + strand, within RS_RS13225at 2842.495 kb on - strand, within RS_RS13225at 2842.541 kb on - strandat 2842.579 kb on - strandat 2842.640 kb on + strand, within RS_RS13230at 2842.641 kb on - strand, within RS_RS13230at 2842.700 kb on + strand, within RS_RS13230at 2842.701 kb on - strand, within RS_RS13230

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
remove
2,840,713 + +0.0
2,840,713 + -0.2
2,840,713 + +0.0
2,840,714 - -0.2
2,840,714 - +0.0
2,841,436 - +0.5
2,842,247 - RS_RS25585 0.67 +0.3
2,842,254 + RS_RS25585 0.70 -0.1
2,842,255 - RS_RS25585 0.71 -0.1
2,842,255 - RS_RS25585 0.71 -0.1
2,842,310 + -0.4
2,842,310 + -0.5
2,842,310 + +0.0
2,842,310 + -0.2
2,842,310 + +0.1
2,842,311 - -0.1
2,842,311 - +0.4
2,842,311 - -0.4
2,842,344 + -0.1
2,842,345 - -0.2
2,842,345 - -0.7
2,842,359 - +0.1
2,842,377 - +1.2
2,842,494 + RS_RS13225 0.70 -1.2
2,842,495 - RS_RS13225 0.70 -1.2
2,842,541 - -0.1
2,842,579 - -0.2
2,842,640 + RS_RS13230 0.19 +0.2
2,842,641 - RS_RS13230 0.19 -1.4
2,842,700 + RS_RS13230 0.34 -0.3
2,842,701 - RS_RS13230 0.35 +0.8

Or see this region's nucleotide sequence