Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS20845 and RS_RS20850 overlap by 14 nucleotides RS_RS20850 and RS_RS20855 overlap by 4 nucleotides RS_RS20855 and RS_RS20860 overlap by 17 nucleotides
RS_RS20845: RS_RS20845 - PLP-dependent transferase, at 982,886 to 984,427
_RS20845
RS_RS20850: RS_RS20850 - aminoacyl-tRNA synthet, at 984,414 to 986,366
_RS20850
RS_RS20855: RS_RS20855 - acyl carrier protein, at 986,363 to 986,632
_RS20855
RS_RS20860: RS_RS20860 - omega-3 fatty acid desaturase, at 986,616 to 987,593
_RS20860
Position (kb) 984
985
986
987 Strain fitness (log2 ratio) -1
0
1 at 983.504 kb on + strand, within RS_RS20845 at 983.504 kb on + strand, within RS_RS20845 at 983.504 kb on + strand, within RS_RS20845 at 983.504 kb on + strand, within RS_RS20845 at 983.505 kb on - strand, within RS_RS20845 at 983.505 kb on - strand, within RS_RS20845 at 983.511 kb on - strand, within RS_RS20845 at 983.511 kb on - strand, within RS_RS20845 at 983.573 kb on + strand, within RS_RS20845 at 983.573 kb on + strand, within RS_RS20845 at 983.574 kb on - strand, within RS_RS20845 at 983.590 kb on - strand, within RS_RS20845 at 983.801 kb on + strand, within RS_RS20845 at 983.802 kb on - strand, within RS_RS20845 at 983.802 kb on - strand, within RS_RS20845 at 983.802 kb on - strand, within RS_RS20845 at 983.802 kb on - strand, within RS_RS20845 at 983.804 kb on + strand, within RS_RS20845 at 983.804 kb on + strand, within RS_RS20845 at 983.805 kb on - strand, within RS_RS20845 at 983.805 kb on - strand, within RS_RS20845 at 983.822 kb on + strand, within RS_RS20845 at 983.859 kb on - strand, within RS_RS20845 at 984.023 kb on + strand, within RS_RS20845 at 984.023 kb on + strand, within RS_RS20845 at 984.023 kb on + strand, within RS_RS20845 at 984.024 kb on - strand, within RS_RS20845 at 984.024 kb on - strand, within RS_RS20845 at 984.069 kb on - strand, within RS_RS20845 at 984.164 kb on + strand, within RS_RS20845 at 984.305 kb on + strand at 984.306 kb on - strand at 984.306 kb on - strand at 984.392 kb on + strand at 984.393 kb on - strand at 984.393 kb on - strand at 984.393 kb on - strand at 984.568 kb on - strand at 984.568 kb on - strand at 984.577 kb on - strand at 984.651 kb on + strand, within RS_RS20850 at 984.651 kb on + strand, within RS_RS20850 at 984.652 kb on - strand, within RS_RS20850 at 984.843 kb on + strand, within RS_RS20850 at 984.843 kb on + strand, within RS_RS20850 at 984.844 kb on - strand, within RS_RS20850 at 984.844 kb on - strand, within RS_RS20850 at 984.844 kb on - strand, within RS_RS20850 at 984.849 kb on + strand, within RS_RS20850 at 984.849 kb on + strand, within RS_RS20850 at 984.849 kb on + strand, within RS_RS20850 at 984.850 kb on - strand, within RS_RS20850 at 984.850 kb on - strand, within RS_RS20850 at 984.991 kb on - strand, within RS_RS20850 at 985.215 kb on + strand, within RS_RS20850 at 985.215 kb on + strand, within RS_RS20850 at 985.215 kb on + strand, within RS_RS20850 at 985.215 kb on + strand, within RS_RS20850 at 985.216 kb on - strand, within RS_RS20850 at 985.216 kb on - strand, within RS_RS20850 at 985.216 kb on - strand, within RS_RS20850 at 985.216 kb on - strand, within RS_RS20850 at 985.216 kb on - strand, within RS_RS20850 at 985.407 kb on + strand, within RS_RS20850 at 985.407 kb on + strand, within RS_RS20850 at 985.407 kb on + strand, within RS_RS20850 at 985.823 kb on - strand, within RS_RS20850 at 985.824 kb on + strand, within RS_RS20850 at 985.824 kb on + strand, within RS_RS20850 at 985.824 kb on + strand, within RS_RS20850 at 985.824 kb on + strand, within RS_RS20850 at 985.825 kb on - strand, within RS_RS20850 at 985.825 kb on - strand, within RS_RS20850 at 985.825 kb on - strand, within RS_RS20850 at 985.825 kb on - strand, within RS_RS20850 at 985.825 kb on - strand, within RS_RS20850 at 985.830 kb on + strand, within RS_RS20850 at 985.831 kb on - strand, within RS_RS20850 at 985.831 kb on - strand, within RS_RS20850 at 986.016 kb on + strand, within RS_RS20850 at 986.016 kb on + strand, within RS_RS20850 at 986.017 kb on - strand, within RS_RS20850 at 986.754 kb on + strand, within RS_RS20860 at 986.755 kb on - strand, within RS_RS20860 at 987.051 kb on + strand, within RS_RS20860 at 987.052 kb on - strand, within RS_RS20860 at 987.099 kb on + strand, within RS_RS20860 at 987.309 kb on + strand, within RS_RS20860 at 987.333 kb on + strand, within RS_RS20860 at 987.333 kb on + strand, within RS_RS20860 at 987.333 kb on + strand, within RS_RS20860 at 987.334 kb on - strand, within RS_RS20860
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 983,504 + RS_RS20845 0.40 +0.1 983,504 + RS_RS20845 0.40 +0.2 983,504 + RS_RS20845 0.40 -0.2 983,504 + RS_RS20845 0.40 -0.5 983,505 - RS_RS20845 0.40 +0.2 983,505 - RS_RS20845 0.40 +0.5 983,511 - RS_RS20845 0.41 -0.0 983,511 - RS_RS20845 0.41 +0.3 983,573 + RS_RS20845 0.45 -0.9 983,573 + RS_RS20845 0.45 +1.2 983,574 - RS_RS20845 0.45 -0.3 983,590 - RS_RS20845 0.46 -0.5 983,801 + RS_RS20845 0.59 +0.5 983,802 - RS_RS20845 0.59 -0.1 983,802 - RS_RS20845 0.59 +0.1 983,802 - RS_RS20845 0.59 +0.4 983,802 - RS_RS20845 0.59 +0.1 983,804 + RS_RS20845 0.60 -0.2 983,804 + RS_RS20845 0.60 +0.1 983,805 - RS_RS20845 0.60 -0.2 983,805 - RS_RS20845 0.60 +1.5 983,822 + RS_RS20845 0.61 +0.1 983,859 - RS_RS20845 0.63 +0.1 984,023 + RS_RS20845 0.74 +0.5 984,023 + RS_RS20845 0.74 +0.4 984,023 + RS_RS20845 0.74 -0.2 984,024 - RS_RS20845 0.74 -0.1 984,024 - RS_RS20845 0.74 +0.5 984,069 - RS_RS20845 0.77 +0.6 984,164 + RS_RS20845 0.83 -1.4 984,305 + -0.3 984,306 - +0.1 984,306 - +0.3 984,392 + -0.5 984,393 - -0.9 984,393 - +0.0 984,393 - -0.4 984,568 - -0.9 984,568 - +0.5 984,577 - +1.2 984,651 + RS_RS20850 0.12 -0.0 984,651 + RS_RS20850 0.12 -0.0 984,652 - RS_RS20850 0.12 -0.7 984,843 + RS_RS20850 0.22 -0.0 984,843 + RS_RS20850 0.22 -0.5 984,844 - RS_RS20850 0.22 -0.1 984,844 - RS_RS20850 0.22 +0.2 984,844 - RS_RS20850 0.22 -0.5 984,849 + RS_RS20850 0.22 +0.2 984,849 + RS_RS20850 0.22 -0.7 984,849 + RS_RS20850 0.22 -0.1 984,850 - RS_RS20850 0.22 +0.7 984,850 - RS_RS20850 0.22 +0.5 984,991 - RS_RS20850 0.30 +0.5 985,215 + RS_RS20850 0.41 -0.4 985,215 + RS_RS20850 0.41 +0.2 985,215 + RS_RS20850 0.41 -0.9 985,215 + RS_RS20850 0.41 -0.1 985,216 - RS_RS20850 0.41 -0.2 985,216 - RS_RS20850 0.41 -0.1 985,216 - RS_RS20850 0.41 +0.2 985,216 - RS_RS20850 0.41 +0.1 985,216 - RS_RS20850 0.41 -0.6 985,407 + RS_RS20850 0.51 -1.1 985,407 + RS_RS20850 0.51 -0.5 985,407 + RS_RS20850 0.51 -0.0 985,823 - RS_RS20850 0.72 -0.6 985,824 + RS_RS20850 0.72 +0.1 985,824 + RS_RS20850 0.72 -0.5 985,824 + RS_RS20850 0.72 -0.3 985,824 + RS_RS20850 0.72 -0.5 985,825 - RS_RS20850 0.72 -0.5 985,825 - RS_RS20850 0.72 -0.1 985,825 - RS_RS20850 0.72 -0.1 985,825 - RS_RS20850 0.72 -0.3 985,825 - RS_RS20850 0.72 +0.4 985,830 + RS_RS20850 0.73 -0.4 985,831 - RS_RS20850 0.73 +0.0 985,831 - RS_RS20850 0.73 -0.4 986,016 + RS_RS20850 0.82 -0.1 986,016 + RS_RS20850 0.82 -1.1 986,017 - RS_RS20850 0.82 -0.8 986,754 + RS_RS20860 0.14 +0.7 986,755 - RS_RS20860 0.14 +0.1 987,051 + RS_RS20860 0.44 -0.1 987,052 - RS_RS20860 0.45 +1.2 987,099 + RS_RS20860 0.49 +0.0 987,309 + RS_RS20860 0.71 +0.5 987,333 + RS_RS20860 0.73 -0.6 987,333 + RS_RS20860 0.73 -0.5 987,333 + RS_RS20860 0.73 -0.1 987,334 - RS_RS20860 0.73 +0.3
Or see this region's nucleotide sequence