Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS12880

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS12870 and RS_RS12875 are separated by 10 nucleotidesRS_RS12875 and RS_RS12880 are separated by 1 nucleotidesRS_RS12880 and RS_RS12885 are separated by 100 nucleotides RS_RS12870: RS_RS12870 - prolyl 4-hydroxylase, at 2,776,088 to 2,776,840 _RS12870 RS_RS12875: RS_RS12875 - DNA oxidative demethylase AlkB, at 2,776,851 to 2,777,507 _RS12875 RS_RS12880: RS_RS12880 - DNA-3-methyladenine glycosylase 2 family protein, at 2,777,509 to 2,778,147 _RS12880 RS_RS12885: RS_RS12885 - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 2,778,248 to 2,779,312 _RS12885 Position (kb) 2777 2778 2779Strain fitness (log2 ratio) -1 0 1 2at 2776.520 kb on + strand, within RS_RS12870at 2776.520 kb on + strand, within RS_RS12870at 2776.520 kb on + strand, within RS_RS12870at 2776.535 kb on + strand, within RS_RS12870at 2776.559 kb on + strand, within RS_RS12870at 2776.807 kb on + strandat 2776.807 kb on + strandat 2776.807 kb on + strandat 2776.807 kb on + strandat 2777.230 kb on - strand, within RS_RS12875at 2777.542 kb on + strandat 2777.968 kb on - strand, within RS_RS12880at 2778.100 kb on + strandat 2778.121 kb on + strandat 2778.122 kb on - strandat 2778.122 kb on - strandat 2778.218 kb on + strandat 2778.218 kb on + strandat 2778.218 kb on + strandat 2778.218 kb on + strandat 2778.219 kb on - strandat 2778.219 kb on - strandat 2778.339 kb on - strandat 2778.362 kb on + strand, within RS_RS12885at 2778.449 kb on + strand, within RS_RS12885at 2778.719 kb on + strand, within RS_RS12885at 2778.752 kb on + strand, within RS_RS12885at 2778.752 kb on + strand, within RS_RS12885at 2778.753 kb on - strand, within RS_RS12885at 2778.753 kb on - strand, within RS_RS12885

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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2,776,520 + RS_RS12870 0.57 +0.1
2,776,520 + RS_RS12870 0.57 -0.1
2,776,520 + RS_RS12870 0.57 -0.1
2,776,535 + RS_RS12870 0.59 +0.1
2,776,559 + RS_RS12870 0.63 +0.1
2,776,807 + -0.0
2,776,807 + +1.9
2,776,807 + +0.0
2,776,807 + +0.1
2,777,230 - RS_RS12875 0.58 -0.2
2,777,542 + -0.1
2,777,968 - RS_RS12880 0.72 +0.2
2,778,100 + +0.1
2,778,121 + -0.0
2,778,122 - -0.0
2,778,122 - -0.3
2,778,218 + +0.9
2,778,218 + -1.1
2,778,218 + +0.7
2,778,218 + +0.2
2,778,219 - +0.2
2,778,219 - +0.1
2,778,339 - +0.9
2,778,362 + RS_RS12885 0.11 -0.3
2,778,449 + RS_RS12885 0.19 -0.0
2,778,719 + RS_RS12885 0.44 -0.9
2,778,752 + RS_RS12885 0.47 +0.2
2,778,752 + RS_RS12885 0.47 +0.1
2,778,753 - RS_RS12885 0.47 -0.1
2,778,753 - RS_RS12885 0.47 +0.1

Or see this region's nucleotide sequence