Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS12870 and RS_RS12875 are separated by 10 nucleotides RS_RS12875 and RS_RS12880 are separated by 1 nucleotides RS_RS12880 and RS_RS12885 are separated by 100 nucleotides
RS_RS12870: RS_RS12870 - prolyl 4-hydroxylase, at 2,776,088 to 2,776,840
_RS12870
RS_RS12875: RS_RS12875 - DNA oxidative demethylase AlkB, at 2,776,851 to 2,777,507
_RS12875
RS_RS12880: RS_RS12880 - DNA-3-methyladenine glycosylase 2 family protein, at 2,777,509 to 2,778,147
_RS12880
RS_RS12885: RS_RS12885 - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 2,778,248 to 2,779,312
_RS12885
Position (kb)
2777
2778
2779 Strain fitness (log2 ratio)
-1
0
1
2 at 2776.520 kb on + strand, within RS_RS12870 at 2776.520 kb on + strand, within RS_RS12870 at 2776.520 kb on + strand, within RS_RS12870 at 2776.535 kb on + strand, within RS_RS12870 at 2776.559 kb on + strand, within RS_RS12870 at 2776.807 kb on + strand at 2776.807 kb on + strand at 2776.807 kb on + strand at 2776.807 kb on + strand at 2777.230 kb on - strand, within RS_RS12875 at 2777.542 kb on + strand at 2777.968 kb on - strand, within RS_RS12880 at 2778.100 kb on + strand at 2778.121 kb on + strand at 2778.122 kb on - strand at 2778.122 kb on - strand at 2778.218 kb on + strand at 2778.218 kb on + strand at 2778.218 kb on + strand at 2778.218 kb on + strand at 2778.219 kb on - strand at 2778.219 kb on - strand at 2778.339 kb on - strand at 2778.362 kb on + strand, within RS_RS12885 at 2778.449 kb on + strand, within RS_RS12885 at 2778.719 kb on + strand, within RS_RS12885 at 2778.752 kb on + strand, within RS_RS12885 at 2778.752 kb on + strand, within RS_RS12885 at 2778.753 kb on - strand, within RS_RS12885 at 2778.753 kb on - strand, within RS_RS12885
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 2,776,520 + RS_RS12870 0.57 +0.1 2,776,520 + RS_RS12870 0.57 -0.1 2,776,520 + RS_RS12870 0.57 -0.1 2,776,535 + RS_RS12870 0.59 +0.1 2,776,559 + RS_RS12870 0.63 +0.1 2,776,807 + -0.0 2,776,807 + +1.9 2,776,807 + +0.0 2,776,807 + +0.1 2,777,230 - RS_RS12875 0.58 -0.2 2,777,542 + -0.1 2,777,968 - RS_RS12880 0.72 +0.2 2,778,100 + +0.1 2,778,121 + -0.0 2,778,122 - -0.0 2,778,122 - -0.3 2,778,218 + +0.9 2,778,218 + -1.1 2,778,218 + +0.7 2,778,218 + +0.2 2,778,219 - +0.2 2,778,219 - +0.1 2,778,339 - +0.9 2,778,362 + RS_RS12885 0.11 -0.3 2,778,449 + RS_RS12885 0.19 -0.0 2,778,719 + RS_RS12885 0.44 -0.9 2,778,752 + RS_RS12885 0.47 +0.2 2,778,752 + RS_RS12885 0.47 +0.1 2,778,753 - RS_RS12885 0.47 -0.1 2,778,753 - RS_RS12885 0.47 +0.1
Or see this region's nucleotide sequence