Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01125 and RS_RS25275 are separated by 29 nucleotides RS_RS25275 and RS_RS01130 overlap by 4 nucleotides RS_RS01130 and RS_RS01135 are separated by 7 nucleotides
RS_RS01125: RS_RS01125 - PLP-dependent aminotransferase family protein, at 250,678 to 251,886
_RS01125
RS_RS25275: RS_RS25275 - hypothetical protein, at 251,916 to 252,101
_RS25275
RS_RS01130: RS_RS01130 - membrane protein, at 252,098 to 253,294
_RS01130
RS_RS01135: RS_RS01135 - LysE family translocator, at 253,302 to 253,937
_RS01135
Position (kb) 252
253
254 Strain fitness (log2 ratio) -1
0
1 at 251.185 kb on + strand, within RS_RS01125 at 251.185 kb on + strand, within RS_RS01125 at 251.186 kb on - strand, within RS_RS01125 at 251.186 kb on - strand, within RS_RS01125 at 251.299 kb on + strand, within RS_RS01125 at 251.300 kb on - strand, within RS_RS01125 at 251.311 kb on + strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125 at 251.387 kb on - strand, within RS_RS01125 at 251.585 kb on - strand, within RS_RS01125 at 251.767 kb on + strand at 251.767 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.806 kb on + strand at 251.807 kb on - strand at 251.807 kb on - strand at 251.844 kb on - strand at 252.150 kb on + strand at 252.150 kb on + strand at 252.151 kb on - strand at 252.151 kb on - strand at 252.151 kb on - strand at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.450 kb on + strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.451 kb on - strand, within RS_RS01130 at 252.456 kb on + strand, within RS_RS01130 at 252.457 kb on - strand, within RS_RS01130 at 252.457 kb on - strand, within RS_RS01130 at 252.534 kb on + strand, within RS_RS01130 at 252.534 kb on + strand, within RS_RS01130 at 252.535 kb on - strand, within RS_RS01130 at 252.535 kb on - strand, within RS_RS01130 at 252.535 kb on - strand, within RS_RS01130 at 252.741 kb on + strand, within RS_RS01130 at 252.742 kb on - strand, within RS_RS01130 at 252.954 kb on + strand, within RS_RS01130 at 252.954 kb on + strand, within RS_RS01130 at 252.955 kb on - strand, within RS_RS01130 at 252.955 kb on - strand, within RS_RS01130 at 252.955 kb on - strand, within RS_RS01130 at 253.430 kb on - strand, within RS_RS01135 at 253.685 kb on - strand, within RS_RS01135 at 253.685 kb on - strand, within RS_RS01135 at 253.693 kb on + strand, within RS_RS01135 at 253.694 kb on - strand, within RS_RS01135 at 253.694 kb on - strand, within RS_RS01135 at 253.694 kb on - strand, within RS_RS01135 at 253.788 kb on - strand, within RS_RS01135 at 253.981 kb on + strand at 253.981 kb on + strand at 253.982 kb on - strand at 253.982 kb on - strand at 253.982 kb on - strand at 253.982 kb on - strand at 253.982 kb on - strand at 254.163 kb on + strand at 254.177 kb on + strand at 254.178 kb on - strand at 254.197 kb on - strand at 254.278 kb on + strand at 254.279 kb on - strand at 254.287 kb on - strand at 254.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 251,185 + RS_RS01125 0.42 +0.2 251,185 + RS_RS01125 0.42 -1.2 251,186 - RS_RS01125 0.42 +0.3 251,186 - RS_RS01125 0.42 +0.3 251,299 + RS_RS01125 0.51 -1.4 251,300 - RS_RS01125 0.51 -0.3 251,311 + RS_RS01125 0.52 -0.3 251,312 - RS_RS01125 0.52 +0.0 251,312 - RS_RS01125 0.52 +0.2 251,312 - RS_RS01125 0.52 +0.0 251,387 - RS_RS01125 0.59 +0.4 251,585 - RS_RS01125 0.75 -0.9 251,767 + +0.0 251,767 + +0.1 251,806 + +0.1 251,806 + +0.0 251,806 + -0.0 251,806 + +1.2 251,806 + +0.6 251,806 + +0.1 251,806 + -0.7 251,807 - +0.4 251,807 - -0.1 251,844 - +0.2 252,150 + +0.0 252,150 + +0.9 252,151 - +0.9 252,151 - +0.7 252,151 - +0.2 252,450 + RS_RS01130 0.29 -0.1 252,450 + RS_RS01130 0.29 -0.2 252,450 + RS_RS01130 0.29 +0.3 252,450 + RS_RS01130 0.29 -0.1 252,450 + RS_RS01130 0.29 +0.1 252,450 + RS_RS01130 0.29 +0.7 252,450 + RS_RS01130 0.29 +0.5 252,450 + RS_RS01130 0.29 -0.1 252,451 - RS_RS01130 0.29 -0.1 252,451 - RS_RS01130 0.29 +0.6 252,451 - RS_RS01130 0.29 +0.1 252,451 - RS_RS01130 0.29 -0.2 252,451 - RS_RS01130 0.29 +1.0 252,451 - RS_RS01130 0.29 -0.4 252,456 + RS_RS01130 0.30 +0.1 252,457 - RS_RS01130 0.30 +0.2 252,457 - RS_RS01130 0.30 +0.0 252,534 + RS_RS01130 0.36 -0.6 252,534 + RS_RS01130 0.36 -0.8 252,535 - RS_RS01130 0.37 +0.0 252,535 - RS_RS01130 0.37 -0.2 252,535 - RS_RS01130 0.37 -0.3 252,741 + RS_RS01130 0.54 +0.2 252,742 - RS_RS01130 0.54 -1.1 252,954 + RS_RS01130 0.72 -0.6 252,954 + RS_RS01130 0.72 -0.1 252,955 - RS_RS01130 0.72 -0.0 252,955 - RS_RS01130 0.72 +0.0 252,955 - RS_RS01130 0.72 -0.3 253,430 - RS_RS01135 0.20 +0.0 253,685 - RS_RS01135 0.60 -0.4 253,685 - RS_RS01135 0.60 -0.3 253,693 + RS_RS01135 0.61 -0.0 253,694 - RS_RS01135 0.62 +0.0 253,694 - RS_RS01135 0.62 -0.4 253,694 - RS_RS01135 0.62 -1.3 253,788 - RS_RS01135 0.76 -0.7 253,981 + +0.3 253,981 + -0.3 253,982 - -0.1 253,982 - -0.1 253,982 - -0.4 253,982 - -0.1 253,982 - +0.2 254,163 + +0.7 254,177 + -0.2 254,178 - +0.7 254,197 - +0.2 254,278 + +1.2 254,279 - -0.2 254,287 - +1.0 254,287 - -0.3
Or see this region's nucleotide sequence