Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS18240 and RS_RS18245 are separated by 15 nucleotides RS_RS18245 and RS_RS18250 are separated by 15 nucleotides
RS_RS18240: RS_RS18240 - salicylaldehyde dehydrogenase, at 280,919 to 282,373
_RS18240
RS_RS18245: RS_RS18245 - feruloyl-CoA synthase, at 282,389 to 284,263
_RS18245
RS_RS18250: RS_RS18250 - sigma-54-dependent Fis family transcriptional regulator, at 284,279 to 286,150
_RS18250
Position (kb) 282
283
284
285 Strain fitness (log2 ratio) -1
0
1
2 at 282.047 kb on + strand, within RS_RS18240 at 282.134 kb on + strand, within RS_RS18240 at 282.218 kb on - strand, within RS_RS18240 at 282.623 kb on + strand, within RS_RS18245 at 282.623 kb on + strand, within RS_RS18245 at 282.623 kb on + strand, within RS_RS18245 at 282.624 kb on - strand, within RS_RS18245 at 282.755 kb on + strand, within RS_RS18245 at 282.755 kb on + strand, within RS_RS18245 at 282.755 kb on + strand, within RS_RS18245 at 282.755 kb on + strand, within RS_RS18245 at 282.755 kb on + strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.756 kb on - strand, within RS_RS18245 at 282.771 kb on - strand, within RS_RS18245 at 282.812 kb on + strand, within RS_RS18245 at 282.812 kb on + strand, within RS_RS18245 at 282.813 kb on - strand, within RS_RS18245 at 282.827 kb on + strand, within RS_RS18245 at 282.860 kb on + strand, within RS_RS18245 at 282.860 kb on + strand, within RS_RS18245 at 282.861 kb on - strand, within RS_RS18245 at 282.861 kb on - strand, within RS_RS18245 at 283.232 kb on + strand, within RS_RS18245 at 283.232 kb on + strand, within RS_RS18245 at 283.233 kb on - strand, within RS_RS18245 at 283.233 kb on - strand, within RS_RS18245 at 283.247 kb on + strand, within RS_RS18245 at 283.250 kb on + strand, within RS_RS18245 at 283.250 kb on + strand, within RS_RS18245 at 283.251 kb on - strand, within RS_RS18245 at 283.251 kb on - strand, within RS_RS18245 at 283.328 kb on + strand, within RS_RS18245 at 283.404 kb on - strand, within RS_RS18245 at 283.404 kb on - strand, within RS_RS18245 at 283.584 kb on - strand, within RS_RS18245 at 283.584 kb on - strand, within RS_RS18245 at 283.680 kb on - strand, within RS_RS18245 at 283.886 kb on + strand, within RS_RS18245 at 284.210 kb on + strand at 284.210 kb on + strand at 284.210 kb on + strand at 284.211 kb on - strand at 284.686 kb on + strand, within RS_RS18250 at 284.703 kb on + strand, within RS_RS18250 at 284.703 kb on + strand, within RS_RS18250 at 284.703 kb on + strand, within RS_RS18250 at 284.703 kb on + strand, within RS_RS18250 at 284.703 kb on + strand, within RS_RS18250 at 284.704 kb on - strand, within RS_RS18250 at 284.704 kb on - strand, within RS_RS18250
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 282,047 + RS_RS18240 0.78 -0.3 282,134 + RS_RS18240 0.84 -0.4 282,218 - RS_RS18240 0.89 -0.5 282,623 + RS_RS18245 0.12 -0.6 282,623 + RS_RS18245 0.12 +0.1 282,623 + RS_RS18245 0.12 -0.1 282,624 - RS_RS18245 0.13 +1.0 282,755 + RS_RS18245 0.20 +0.0 282,755 + RS_RS18245 0.20 +0.5 282,755 + RS_RS18245 0.20 +0.0 282,755 + RS_RS18245 0.20 +0.5 282,755 + RS_RS18245 0.20 -0.6 282,756 - RS_RS18245 0.20 +1.0 282,756 - RS_RS18245 0.20 -0.3 282,756 - RS_RS18245 0.20 +0.3 282,756 - RS_RS18245 0.20 +0.0 282,756 - RS_RS18245 0.20 -0.1 282,756 - RS_RS18245 0.20 -0.4 282,756 - RS_RS18245 0.20 +0.0 282,771 - RS_RS18245 0.20 -0.1 282,812 + RS_RS18245 0.23 +0.2 282,812 + RS_RS18245 0.23 -0.1 282,813 - RS_RS18245 0.23 +0.0 282,827 + RS_RS18245 0.23 +0.1 282,860 + RS_RS18245 0.25 +0.7 282,860 + RS_RS18245 0.25 +0.0 282,861 - RS_RS18245 0.25 -0.5 282,861 - RS_RS18245 0.25 +0.4 283,232 + RS_RS18245 0.45 -0.5 283,232 + RS_RS18245 0.45 +0.2 283,233 - RS_RS18245 0.45 +0.5 283,233 - RS_RS18245 0.45 +0.3 283,247 + RS_RS18245 0.46 +0.6 283,250 + RS_RS18245 0.46 +0.0 283,250 + RS_RS18245 0.46 -0.3 283,251 - RS_RS18245 0.46 -0.9 283,251 - RS_RS18245 0.46 -0.3 283,328 + RS_RS18245 0.50 -1.7 283,404 - RS_RS18245 0.54 -0.3 283,404 - RS_RS18245 0.54 +0.0 283,584 - RS_RS18245 0.64 -0.1 283,584 - RS_RS18245 0.64 +1.0 283,680 - RS_RS18245 0.69 +0.2 283,886 + RS_RS18245 0.80 -0.6 284,210 + +0.0 284,210 + -0.1 284,210 + -0.6 284,211 - +0.6 284,686 + RS_RS18250 0.22 -0.3 284,703 + RS_RS18250 0.23 +0.0 284,703 + RS_RS18250 0.23 -0.5 284,703 + RS_RS18250 0.23 +2.0 284,703 + RS_RS18250 0.23 -0.2 284,703 + RS_RS18250 0.23 -1.0 284,704 - RS_RS18250 0.23 -0.6 284,704 - RS_RS18250 0.23 -0.6
Or see this region's nucleotide sequence