Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17520 and RS_RS17525 are separated by 120 nucleotides RS_RS17525 and RS_RS17530 are separated by 44 nucleotides
RS_RS17520: RS_RS17520 - choline ABC transporter permease subunit, at 76,980 to 77,876
_RS17520
RS_RS17525: RS_RS17525 - GlxA family transcriptional regulator, at 77,997 to 79,001
_RS17525
RS_RS17530: RS_RS17530 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 79,046 to 80,152
_RS17530
Position (kb) 77
78
79
80 Strain fitness (log2 ratio) -2
-1
0
1 at 77.028 kb on + strand at 77.028 kb on + strand at 77.192 kb on + strand, within RS_RS17520 at 77.213 kb on + strand, within RS_RS17520 at 77.213 kb on + strand, within RS_RS17520 at 77.214 kb on - strand, within RS_RS17520 at 77.391 kb on + strand, within RS_RS17520 at 77.462 kb on + strand, within RS_RS17520 at 77.566 kb on - strand, within RS_RS17520 at 77.566 kb on - strand, within RS_RS17520 at 77.866 kb on + strand at 77.867 kb on - strand at 77.955 kb on - strand at 78.144 kb on + strand, within RS_RS17525 at 78.145 kb on - strand, within RS_RS17525 at 78.166 kb on - strand, within RS_RS17525 at 78.180 kb on + strand, within RS_RS17525 at 78.180 kb on + strand, within RS_RS17525 at 78.181 kb on - strand, within RS_RS17525 at 78.354 kb on - strand, within RS_RS17525 at 78.354 kb on - strand, within RS_RS17525 at 78.368 kb on + strand, within RS_RS17525 at 78.646 kb on + strand, within RS_RS17525 at 78.818 kb on - strand, within RS_RS17525 at 78.831 kb on + strand, within RS_RS17525 at 78.831 kb on + strand, within RS_RS17525 at 78.831 kb on + strand, within RS_RS17525 at 78.832 kb on - strand, within RS_RS17525 at 78.832 kb on - strand, within RS_RS17525 at 78.835 kb on - strand, within RS_RS17525 at 78.871 kb on + strand, within RS_RS17525 at 78.872 kb on - strand, within RS_RS17525 at 78.992 kb on - strand at 78.992 kb on - strand at 78.999 kb on + strand at 79.000 kb on - strand at 79.014 kb on + strand at 79.015 kb on - strand at 79.176 kb on - strand, within RS_RS17530 at 79.178 kb on + strand, within RS_RS17530 at 79.178 kb on + strand, within RS_RS17530 at 79.179 kb on - strand, within RS_RS17530 at 79.179 kb on - strand, within RS_RS17530 at 79.179 kb on - strand, within RS_RS17530 at 79.179 kb on - strand, within RS_RS17530 at 79.307 kb on + strand, within RS_RS17530 at 79.308 kb on - strand, within RS_RS17530 at 79.308 kb on - strand, within RS_RS17530 at 79.448 kb on + strand, within RS_RS17530 at 79.448 kb on + strand, within RS_RS17530 at 79.541 kb on + strand, within RS_RS17530 at 79.541 kb on + strand, within RS_RS17530 at 79.583 kb on + strand, within RS_RS17530 at 79.583 kb on + strand, within RS_RS17530 at 79.584 kb on - strand, within RS_RS17530 at 79.789 kb on + strand, within RS_RS17530
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 77,028 + -0.6 77,028 + -0.1 77,192 + RS_RS17520 0.24 +0.1 77,213 + RS_RS17520 0.26 -1.8 77,213 + RS_RS17520 0.26 +1.0 77,214 - RS_RS17520 0.26 -0.2 77,391 + RS_RS17520 0.46 +0.1 77,462 + RS_RS17520 0.54 -0.0 77,566 - RS_RS17520 0.65 -0.9 77,566 - RS_RS17520 0.65 -0.2 77,866 + -0.2 77,867 - -1.3 77,955 - +0.7 78,144 + RS_RS17525 0.15 +0.3 78,145 - RS_RS17525 0.15 +0.4 78,166 - RS_RS17525 0.17 +0.4 78,180 + RS_RS17525 0.18 +0.0 78,180 + RS_RS17525 0.18 +0.7 78,181 - RS_RS17525 0.18 -0.0 78,354 - RS_RS17525 0.36 +1.6 78,354 - RS_RS17525 0.36 -0.3 78,368 + RS_RS17525 0.37 -0.2 78,646 + RS_RS17525 0.65 -1.3 78,818 - RS_RS17525 0.82 +0.6 78,831 + RS_RS17525 0.83 +0.4 78,831 + RS_RS17525 0.83 +1.0 78,831 + RS_RS17525 0.83 +0.4 78,832 - RS_RS17525 0.83 +0.6 78,832 - RS_RS17525 0.83 +0.6 78,835 - RS_RS17525 0.83 +0.0 78,871 + RS_RS17525 0.87 +0.3 78,872 - RS_RS17525 0.87 +0.0 78,992 - +0.0 78,992 - -0.6 78,999 + -0.1 79,000 - -0.4 79,014 + +0.1 79,015 - +0.0 79,176 - RS_RS17530 0.12 +0.3 79,178 + RS_RS17530 0.12 -0.6 79,178 + RS_RS17530 0.12 +0.1 79,179 - RS_RS17530 0.12 -0.1 79,179 - RS_RS17530 0.12 -1.1 79,179 - RS_RS17530 0.12 +0.2 79,179 - RS_RS17530 0.12 -0.3 79,307 + RS_RS17530 0.24 +0.2 79,308 - RS_RS17530 0.24 +1.0 79,308 - RS_RS17530 0.24 +0.3 79,448 + RS_RS17530 0.36 -0.1 79,448 + RS_RS17530 0.36 +0.3 79,541 + RS_RS17530 0.45 -0.7 79,541 + RS_RS17530 0.45 -0.7 79,583 + RS_RS17530 0.49 +0.6 79,583 + RS_RS17530 0.49 -0.2 79,584 - RS_RS17530 0.49 +0.1 79,789 + RS_RS17530 0.67 +1.0
Or see this region's nucleotide sequence