Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01650 and RS_RS01655 are separated by 141 nucleotides RS_RS01655 and RS_RS01660 are separated by 46 nucleotides RS_RS01660 and RS_RS01665 are separated by 143 nucleotides
RS_RS01650: RS_RS01650 - hypothetical protein, at 367,224 to 367,784
_RS01650
RS_RS01655: RS_RS01655 - hypothetical protein, at 367,926 to 368,402
_RS01655
RS_RS01660: RS_RS01660 - polyphenol oxidase, at 368,449 to 369,939
_RS01660
RS_RS01665: RS_RS01665 - ATP-dependent protease, at 370,083 to 371,615
_RS01665
Position (kb) 368
369
370 Strain fitness (log2 ratio) -1
0
1 at 367.689 kb on + strand, within RS_RS01650 at 367.690 kb on - strand, within RS_RS01650 at 367.690 kb on - strand, within RS_RS01650 at 367.779 kb on + strand at 367.938 kb on - strand at 368.045 kb on - strand, within RS_RS01655 at 368.101 kb on + strand, within RS_RS01655 at 368.102 kb on - strand, within RS_RS01655 at 368.159 kb on - strand, within RS_RS01655 at 368.159 kb on - strand, within RS_RS01655 at 368.297 kb on + strand, within RS_RS01655 at 368.297 kb on + strand, within RS_RS01655 at 368.298 kb on - strand, within RS_RS01655 at 368.298 kb on - strand, within RS_RS01655 at 368.735 kb on + strand, within RS_RS01660 at 368.735 kb on + strand, within RS_RS01660 at 368.778 kb on + strand, within RS_RS01660 at 368.778 kb on + strand, within RS_RS01660 at 368.778 kb on + strand, within RS_RS01660 at 368.778 kb on + strand, within RS_RS01660 at 368.779 kb on - strand, within RS_RS01660 at 368.908 kb on - strand, within RS_RS01660 at 369.062 kb on + strand, within RS_RS01660 at 369.062 kb on + strand, within RS_RS01660 at 369.063 kb on - strand, within RS_RS01660 at 369.086 kb on + strand, within RS_RS01660 at 369.086 kb on + strand, within RS_RS01660 at 369.086 kb on + strand, within RS_RS01660 at 369.086 kb on + strand, within RS_RS01660 at 369.087 kb on - strand, within RS_RS01660 at 369.087 kb on - strand, within RS_RS01660 at 369.401 kb on + strand, within RS_RS01660 at 369.401 kb on + strand, within RS_RS01660 at 369.486 kb on - strand, within RS_RS01660 at 369.510 kb on - strand, within RS_RS01660 at 369.510 kb on - strand, within RS_RS01660 at 369.584 kb on + strand, within RS_RS01660 at 369.614 kb on + strand, within RS_RS01660 at 369.615 kb on - strand, within RS_RS01660 at 369.650 kb on + strand, within RS_RS01660 at 369.650 kb on + strand, within RS_RS01660 at 369.651 kb on - strand, within RS_RS01660 at 369.651 kb on - strand, within RS_RS01660 at 369.671 kb on + strand, within RS_RS01660 at 369.672 kb on - strand, within RS_RS01660 at 369.672 kb on - strand, within RS_RS01660 at 369.677 kb on + strand, within RS_RS01660 at 369.678 kb on - strand, within RS_RS01660 at 369.693 kb on + strand, within RS_RS01660 at 369.694 kb on - strand, within RS_RS01660 at 369.883 kb on + strand at 369.927 kb on + strand at 369.928 kb on - strand at 369.928 kb on - strand at 370.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 367,689 + RS_RS01650 0.83 -0.6 367,690 - RS_RS01650 0.83 -0.4 367,690 - RS_RS01650 0.83 -0.3 367,779 + +0.7 367,938 - -0.4 368,045 - RS_RS01655 0.25 -1.0 368,101 + RS_RS01655 0.37 +0.0 368,102 - RS_RS01655 0.37 -0.3 368,159 - RS_RS01655 0.49 +0.2 368,159 - RS_RS01655 0.49 -0.4 368,297 + RS_RS01655 0.78 -0.4 368,297 + RS_RS01655 0.78 -0.1 368,298 - RS_RS01655 0.78 +1.3 368,298 - RS_RS01655 0.78 -0.4 368,735 + RS_RS01660 0.19 +0.2 368,735 + RS_RS01660 0.19 +0.7 368,778 + RS_RS01660 0.22 -0.4 368,778 + RS_RS01660 0.22 +0.2 368,778 + RS_RS01660 0.22 +0.1 368,778 + RS_RS01660 0.22 -0.1 368,779 - RS_RS01660 0.22 +0.0 368,908 - RS_RS01660 0.31 +0.3 369,062 + RS_RS01660 0.41 +0.3 369,062 + RS_RS01660 0.41 -0.5 369,063 - RS_RS01660 0.41 -0.8 369,086 + RS_RS01660 0.43 -1.2 369,086 + RS_RS01660 0.43 +0.4 369,086 + RS_RS01660 0.43 +0.2 369,086 + RS_RS01660 0.43 -0.2 369,087 - RS_RS01660 0.43 +0.2 369,087 - RS_RS01660 0.43 -0.4 369,401 + RS_RS01660 0.64 -0.4 369,401 + RS_RS01660 0.64 +0.3 369,486 - RS_RS01660 0.70 +1.6 369,510 - RS_RS01660 0.71 +0.2 369,510 - RS_RS01660 0.71 -0.1 369,584 + RS_RS01660 0.76 -0.0 369,614 + RS_RS01660 0.78 -0.4 369,615 - RS_RS01660 0.78 +0.1 369,650 + RS_RS01660 0.81 -0.2 369,650 + RS_RS01660 0.81 +0.3 369,651 - RS_RS01660 0.81 -0.3 369,651 - RS_RS01660 0.81 +0.3 369,671 + RS_RS01660 0.82 -0.3 369,672 - RS_RS01660 0.82 +0.2 369,672 - RS_RS01660 0.82 +0.9 369,677 + RS_RS01660 0.82 +0.0 369,678 - RS_RS01660 0.82 +0.5 369,693 + RS_RS01660 0.83 +0.1 369,694 - RS_RS01660 0.84 +0.5 369,883 + +0.0 369,927 + +0.1 369,928 - -0.4 369,928 - -0.0 370,199 - -0.5
Or see this region's nucleotide sequence