Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS22480 and RS_RS22485 are separated by 304 nucleotides RS_RS22485 and RS_RS22490 are separated by 36 nucleotides
RS_RS22480: RS_RS22480 - alpha/beta hydrolase, at 1,405,471 to 1,406,544
_RS22480
RS_RS22485: RS_RS22485 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 1,406,849 to 1,408,165
_RS22485
RS_RS22490: RS_RS22490 - multidrug efflux RND transporter permease subunit, at 1,408,202 to 1,411,411
_RS22490
Position (kb)
1406
1407
1408
1409 Strain fitness (log2 ratio)
-2
-1
0
1 at 1405.897 kb on + strand, within RS_RS22480 at 1405.945 kb on + strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480 at 1406.203 kb on + strand, within RS_RS22480 at 1406.203 kb on + strand, within RS_RS22480 at 1406.204 kb on - strand, within RS_RS22480 at 1406.237 kb on - strand, within RS_RS22480 at 1406.333 kb on - strand, within RS_RS22480 at 1406.656 kb on + strand at 1406.656 kb on + strand at 1406.657 kb on - strand at 1406.657 kb on - strand at 1406.678 kb on - strand at 1406.678 kb on - strand at 1406.761 kb on + strand at 1406.761 kb on + strand at 1406.762 kb on - strand at 1406.762 kb on - strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.920 kb on - strand at 1407.222 kb on - strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.501 kb on - strand, within RS_RS22485 at 1407.530 kb on + strand, within RS_RS22485 at 1407.530 kb on + strand, within RS_RS22485 at 1407.578 kb on + strand, within RS_RS22485 at 1407.578 kb on + strand, within RS_RS22485 at 1407.578 kb on + strand, within RS_RS22485 at 1407.578 kb on + strand, within RS_RS22485 at 1407.578 kb on + strand, within RS_RS22485 at 1407.579 kb on - strand, within RS_RS22485 at 1407.579 kb on - strand, within RS_RS22485 at 1407.579 kb on - strand, within RS_RS22485 at 1407.579 kb on - strand, within RS_RS22485 at 1407.580 kb on + strand, within RS_RS22485 at 1407.719 kb on + strand, within RS_RS22485 at 1407.719 kb on + strand, within RS_RS22485 at 1407.720 kb on - strand, within RS_RS22485 at 1407.885 kb on + strand, within RS_RS22485 at 1408.436 kb on + strand at 1408.436 kb on + strand at 1408.436 kb on + strand at 1408.436 kb on + strand at 1408.437 kb on - strand at 1408.437 kb on - strand at 1408.437 kb on - strand at 1408.664 kb on + strand, within RS_RS22490 at 1408.664 kb on + strand, within RS_RS22490 at 1408.664 kb on + strand, within RS_RS22490 at 1408.664 kb on + strand, within RS_RS22490 at 1408.664 kb on + strand, within RS_RS22490 at 1408.665 kb on - strand, within RS_RS22490 at 1408.665 kb on - strand, within RS_RS22490 at 1408.665 kb on - strand, within RS_RS22490 at 1408.665 kb on - strand, within RS_RS22490 at 1408.665 kb on - strand, within RS_RS22490 at 1408.676 kb on + strand, within RS_RS22490 at 1408.676 kb on + strand, within RS_RS22490 at 1408.755 kb on - strand, within RS_RS22490
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,405,897 + RS_RS22480 0.40 -0.0 1,405,945 + RS_RS22480 0.44 -0.0 1,405,946 - RS_RS22480 0.44 +0.7 1,405,946 - RS_RS22480 0.44 -0.4 1,406,203 + RS_RS22480 0.68 +0.0 1,406,203 + RS_RS22480 0.68 +0.3 1,406,204 - RS_RS22480 0.68 -0.6 1,406,237 - RS_RS22480 0.71 -0.7 1,406,333 - RS_RS22480 0.80 -0.8 1,406,656 + -0.2 1,406,656 + +0.3 1,406,657 - -0.6 1,406,657 - -0.1 1,406,678 - +0.0 1,406,678 - -0.4 1,406,761 + +0.2 1,406,761 + +0.7 1,406,762 - +1.0 1,406,762 - -0.1 1,406,918 + -0.8 1,406,918 + -0.7 1,406,918 + +0.2 1,406,918 + -0.9 1,406,919 - -0.7 1,406,919 - +1.2 1,406,919 - -0.5 1,406,919 - +0.3 1,406,919 - -1.4 1,406,919 - -1.3 1,406,920 - -0.5 1,407,222 - RS_RS22485 0.28 -0.2 1,407,500 + RS_RS22485 0.49 -0.0 1,407,500 + RS_RS22485 0.49 -0.7 1,407,500 + RS_RS22485 0.49 -0.5 1,407,500 + RS_RS22485 0.49 -1.4 1,407,501 - RS_RS22485 0.50 +0.2 1,407,530 + RS_RS22485 0.52 +0.1 1,407,530 + RS_RS22485 0.52 -0.4 1,407,578 + RS_RS22485 0.55 -0.5 1,407,578 + RS_RS22485 0.55 -0.4 1,407,578 + RS_RS22485 0.55 -0.7 1,407,578 + RS_RS22485 0.55 -0.1 1,407,578 + RS_RS22485 0.55 +0.0 1,407,579 - RS_RS22485 0.55 +0.1 1,407,579 - RS_RS22485 0.55 +0.1 1,407,579 - RS_RS22485 0.55 -0.6 1,407,579 - RS_RS22485 0.55 +0.4 1,407,580 + RS_RS22485 0.56 -0.1 1,407,719 + RS_RS22485 0.66 +0.1 1,407,719 + RS_RS22485 0.66 -0.3 1,407,720 - RS_RS22485 0.66 +0.2 1,407,885 + RS_RS22485 0.79 -0.6 1,408,436 + -1.0 1,408,436 + +0.3 1,408,436 + -0.6 1,408,436 + -0.0 1,408,437 - -0.2 1,408,437 - -0.3 1,408,437 - -0.7 1,408,664 + RS_RS22490 0.14 -0.6 1,408,664 + RS_RS22490 0.14 -1.0 1,408,664 + RS_RS22490 0.14 -0.9 1,408,664 + RS_RS22490 0.14 -1.0 1,408,664 + RS_RS22490 0.14 -0.4 1,408,665 - RS_RS22490 0.14 -0.1 1,408,665 - RS_RS22490 0.14 -0.3 1,408,665 - RS_RS22490 0.14 -0.4 1,408,665 - RS_RS22490 0.14 -1.0 1,408,665 - RS_RS22490 0.14 -2.0 1,408,676 + RS_RS22490 0.15 -0.1 1,408,676 + RS_RS22490 0.15 +0.2 1,408,755 - RS_RS22490 0.17 +0.4
Or see this region's nucleotide sequence