Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS22380

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS22365 and RS_RS22375 overlap by 8 nucleotidesRS_RS22375 and RS_RS22380 are separated by 51 nucleotidesRS_RS22380 and RS_RS22385 are separated by 77 nucleotidesRS_RS22385 and RS_RS22390 are separated by 9 nucleotides RS_RS22365: RS_RS22365 - membrane protein, at 1,379,151 to 1,380,515 _RS22365 RS_RS22375: RS_RS22375 - Flp pilus assembly protein CpaB, at 1,380,508 to 1,381,448 _RS22375 RS_RS22380: RS_RS22380 - pilus assembly protein, at 1,381,500 to 1,381,991 _RS22380 RS_RS22385: RS_RS22385 - peptidase, at 1,382,069 to 1,382,628 _RS22385 RS_RS22390: RS_RS22390 - Flp family type IVb pilin, at 1,382,638 to 1,382,814 _RS22390 Position (kb) 1381 1382Strain fitness (log2 ratio) -1 0 1at 1381.020 kb on + strand, within RS_RS22375at 1381.020 kb on + strand, within RS_RS22375at 1381.020 kb on + strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.021 kb on - strand, within RS_RS22375at 1381.030 kb on + strand, within RS_RS22375at 1381.030 kb on + strand, within RS_RS22375at 1381.031 kb on - strand, within RS_RS22375at 1381.732 kb on + strand, within RS_RS22380at 1381.732 kb on + strand, within RS_RS22380at 1381.733 kb on - strand, within RS_RS22380at 1381.840 kb on + strand, within RS_RS22380at 1381.841 kb on - strand, within RS_RS22380at 1381.841 kb on - strand, within RS_RS22380at 1381.844 kb on - strand, within RS_RS22380at 1381.844 kb on - strand, within RS_RS22380at 1381.844 kb on - strand, within RS_RS22380at 1381.844 kb on - strand, within RS_RS22380at 1382.007 kb on + strandat 1382.070 kb on + strandat 1382.070 kb on + strandat 1382.070 kb on + strandat 1382.070 kb on + strandat 1382.070 kb on + strandat 1382.071 kb on - strandat 1382.071 kb on - strandat 1382.071 kb on - strandat 1382.071 kb on - strandat 1382.071 kb on - strandat 1382.142 kb on + strand, within RS_RS22385at 1382.142 kb on + strand, within RS_RS22385at 1382.142 kb on + strand, within RS_RS22385at 1382.143 kb on - strand, within RS_RS22385at 1382.143 kb on - strand, within RS_RS22385at 1382.143 kb on - strand, within RS_RS22385at 1382.143 kb on - strand, within RS_RS22385at 1382.152 kb on - strand, within RS_RS22385at 1382.152 kb on - strand, within RS_RS22385at 1382.402 kb on + strand, within RS_RS22385at 1382.403 kb on - strand, within RS_RS22385at 1382.462 kb on + strand, within RS_RS22385at 1382.565 kb on + strand, within RS_RS22385at 1382.566 kb on - strand, within RS_RS22385at 1382.566 kb on - strand, within RS_RS22385at 1382.590 kb on + strandat 1382.590 kb on + strandat 1382.591 kb on - strandat 1382.623 kb on - strandat 1382.624 kb on + strandat 1382.624 kb on + strandat 1382.624 kb on + strandat 1382.624 kb on + strandat 1382.624 kb on + strandat 1382.625 kb on - strandat 1382.625 kb on - strandat 1382.625 kb on - strandat 1382.625 kb on - strandat 1382.625 kb on - strandat 1382.625 kb on - strandat 1382.756 kb on + strand, within RS_RS22390at 1382.756 kb on + strand, within RS_RS22390at 1382.757 kb on - strand, within RS_RS22390at 1382.870 kb on + strandat 1382.871 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,381,020 + RS_RS22375 0.54 -0.1
1,381,020 + RS_RS22375 0.54 -0.1
1,381,020 + RS_RS22375 0.54 +0.9
1,381,021 - RS_RS22375 0.55 +0.1
1,381,021 - RS_RS22375 0.55 +0.6
1,381,021 - RS_RS22375 0.55 -0.5
1,381,021 - RS_RS22375 0.55 -0.4
1,381,021 - RS_RS22375 0.55 +0.3
1,381,021 - RS_RS22375 0.55 +0.5
1,381,030 + RS_RS22375 0.55 +0.2
1,381,030 + RS_RS22375 0.55 -0.1
1,381,031 - RS_RS22375 0.56 +1.0
1,381,732 + RS_RS22380 0.47 -1.4
1,381,732 + RS_RS22380 0.47 -0.4
1,381,733 - RS_RS22380 0.47 +0.3
1,381,840 + RS_RS22380 0.69 -0.6
1,381,841 - RS_RS22380 0.69 -0.5
1,381,841 - RS_RS22380 0.69 -0.5
1,381,844 - RS_RS22380 0.70 -0.0
1,381,844 - RS_RS22380 0.70 +0.2
1,381,844 - RS_RS22380 0.70 +0.3
1,381,844 - RS_RS22380 0.70 -1.5
1,382,007 + +0.3
1,382,070 + +0.1
1,382,070 + +0.0
1,382,070 + +1.2
1,382,070 + -0.3
1,382,070 + -0.2
1,382,071 - -1.4
1,382,071 - +0.2
1,382,071 - -0.1
1,382,071 - +1.7
1,382,071 - -0.1
1,382,142 + RS_RS22385 0.13 +0.1
1,382,142 + RS_RS22385 0.13 -0.1
1,382,142 + RS_RS22385 0.13 +0.9
1,382,143 - RS_RS22385 0.13 +0.0
1,382,143 - RS_RS22385 0.13 -0.5
1,382,143 - RS_RS22385 0.13 +1.1
1,382,143 - RS_RS22385 0.13 -0.5
1,382,152 - RS_RS22385 0.15 +1.5
1,382,152 - RS_RS22385 0.15 -0.4
1,382,402 + RS_RS22385 0.59 -0.2
1,382,403 - RS_RS22385 0.60 +0.5
1,382,462 + RS_RS22385 0.70 -1.0
1,382,565 + RS_RS22385 0.89 -1.1
1,382,566 - RS_RS22385 0.89 -0.5
1,382,566 - RS_RS22385 0.89 +0.2
1,382,590 + -1.1
1,382,590 + +0.2
1,382,591 - +0.3
1,382,623 - -0.8
1,382,624 + -0.4
1,382,624 + +0.1
1,382,624 + -0.1
1,382,624 + +0.3
1,382,624 + +0.3
1,382,625 - +0.3
1,382,625 - +0.1
1,382,625 - -0.3
1,382,625 - +0.2
1,382,625 - -0.3
1,382,625 - +0.2
1,382,756 + RS_RS22390 0.67 +0.9
1,382,756 + RS_RS22390 0.67 -0.1
1,382,757 - RS_RS22390 0.67 -0.1
1,382,870 + -0.6
1,382,871 - -0.1

Or see this region's nucleotide sequence